A Road Map for Undertaking Quantitative Proteomics in Plants: New Opportunities for Cereal Crops

Khodabocus, Ibrahim; Li, Qiaomu; Mehta, Devang; Uhrig, R. Glen

doi:10.1007/978-1-0716-1526-3_14

Cited by 1 publication

(1 citation statement)

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“…Previous studies compared modern and ancient wheat species limited to proteins of a specific family such as gluten proteins 32 or ATIs [33][34][35] , or studied the immunogenic potential of ATIs between common wheat and einkorn 21,36 . Compared to earlier gel-based proteomic studies, latest developments in LC-MSbased proteomics allow to quantify thousands of proteins in less than 1.5-2 h per sample 37 . Recently, this proteomic technology was applied to compare the proteomes of few cultivars in common wheat, spelt and rye 38,39 showing that an important number of proteins were differently expressed even between common wheat and spelt, both being hexaploid species.…”

Section: Introductionmentioning

confidence: 99%

Reference proteomes of five wheat species as starting point for future design of cultivars with lower allergenic potential

et al. 2023

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Wheat is an important staple food and its processing quality is largely driven by proteins. However, there is a sizable number of people with inflammatory reactions to wheat proteins, namely celiac disease, wheat allergy and the syndrome of non-celiac wheat sensitivity. Thus, proteome profiles should be of high importance for stakeholders along the wheat supply chain. We applied liquid chromatography-tandem mass spectrometry-based proteomics to establish the flour reference proteome for five wheat species, ancient to modern, each based on 10 cultivars grown in three diverse environments. We identified at least 2540 proteins in each species and a cluster analyses clearly separated the species based on their proteome profiles. Even more, >50% of proteins significantly differed between species - many of them implicated in products’ quality, grain-starch synthesis, plant stress regulation and proven or potential allergic reactions in humans. Notably, the expression of several important wheat proteins was found to be mainly driven by genetics vs. environmental factors, which enables selection and refinement of improved cultivars for the wheat supply chain as long as rapid test methods will be developed. Especially einkorn expressed 5.4 and 7.2-fold lower quantities of potential allergens and immunogenic amylase trypsin inhibitors, respectively, than common wheat, whereas potential allergen content was intermediate in tetraploid wheat species. This urgently warrants well-targeted clinical studies, where the developed reference proteomes will help to design representative test diets.

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