A Risk Score Signature Consisting of Six Immune Genes Predicts Overall Survival in Patients with Lower-Grade Gliomas

Wu, Yongwei; Peng, Zesheng; Gu, Sujie; Hao-fei, Wang; Xiang, Wei

doi:10.1155/2022/2558548

Cited by 3 publications

(3 citation statements)

References 44 publications

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“…constructed an immune risk score signature (IRSS) using the LASSO model, and the IRSS included six relevant immune genes that were good predictors of prognosis in LGG patients. Moreover, the immune infiltration results showed that the genetic profile correlated with innate immune cytopenia ( 12 ). Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

Identification of prognosis-related gene features in low-grade glioma based on ssGSEA

Zhang²,

Tan³

2022

Front. Oncol.

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Low-grade gliomas (LGG) are commonly seen in clinical practice, and the prognosis is often poor. Therefore, the determination of immune-related risk scores and immune-related targets for predicting prognoses in patients with LGG is crucial. A single-sample gene set enrichment analysis (ssGSEA) was performed on 22 immune gene sets to calculate immune-based prognostic scores. The prognostic value of the 22 immune cells for predicting overall survival (OS) was assessed using the least absolute shrinkage and selection operator (LASSO) and univariate and multivariate Cox analyses. Subsequently, we constructed a validated effector T-cell risk score (TCRS) to identify the immune subtypes and inflammatory immune features of LGG patients. We divided an LGG patient into a high-risk–score group and a low-risk–score group based on the optimal cutoff value. Kaplan–Meier survival curve showed that patients in the low-risk–score group had higher OS. We then identified the differentially expressed genes (DEGs) between the high-risk–score group and low-risk-score group and obtained 799 upregulated genes and 348 downregulated genes. The analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) show that DEGs were mainly concentrated in immune-related processes. In order to further explore the immune-related genes related to prognosis, we constructed a protein–protein interaction (PPI) network using Cytoscape and then identified the 50 most crucial genes. Subsequently, nine DEGs were found to be significantly associated with OS based on univariate and multivariate Cox analyses. It was further confirmed that CD2, SPN, IL18, PTPRC, GZMA, and TLR7 were independent prognostic factors for LGG through batch survival analysis and a nomogram prediction model. In addition, we used an RT-qPCR assay to validate the bioinformatics results. The results showed that CD2, SPN, IL18, PTPRC, GZMA, and TLR7 were highly expressed in LGG. Our study can provide a reference value for the prediction of prognosis in LGG patients and may help in the clinical development of effective therapeutic agents.

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Section: Discussionmentioning

confidence: 99%

Identification of prognosis-related gene features in low-grade glioma based on ssGSEA

Zhang²,

Tan³

2022

Front. Oncol.

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“…C-index was calculated to evaluate the consistency of real and predicting survival outcomes. To further verify the performance of CCSPI, we compared different risk systems from other studies ( Supplementary Table S2 ) measured by AUCs and C-index ( Zhang et al, 2020 ; Luo et al, 2021 ; Zhao et al, 2021 ; Zhou et al, 2021 ; Wu et al, 2022 ; Zheng et al, 2022 ) in the CGGA validation cohort.…”

Section: Methodsmentioning

confidence: 99%

A Novel CRISPR/Cas9 Screening Potential Index for Prognostic and Immunological Prediction in Low-Grade Glioma

Xiong

et al. 2022

Front. Genet.

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Glioma is a malignancy with the highest mortality in central nervous system disorders. Here, we implemented the computational tools based on CRISPR/Cas9 to predict the clinical outcomes and biological characteristics of low-grade glioma (LGG). The transcriptional expression profiles and clinical phenotypes of LGG patients were retrieved from The Cancer Genome Atlas and Chinese Glioma Genome Atlas. The CERES algorithm was used to screen for LGG-lethal genes. Cox regression and random survival forest were adopted for survival-related gene selection. Nonnegative matrix factorization distinguished patients into different clusters. Single-sample gene set enrichment analysis was employed to create a novel CRISPR/Cas9 screening potential index (CCSPI), and patients were stratified into low- and high-CCSPI groups. Survival analysis, area under the curve values (AUCs), nomogram, and tumor microenvironment exploration were included for the model validation. A total of 20 essential genes in LGG were used to classify patients into two clusters and construct the CCSPI system. High-CCSPI patients were associated with a worse prognosis of both training and validation set (p < 0.0001) and higher immune fractions than low-CCSPI individuals. The CCSPI system had a promising performance with 1-, 3-, and 5-year AUCs of 0.816, 0.779, 0.724, respectively, and the C-index of the nomogram model reached 0.743 (95% CI = 0.725–0.760). Immune-infiltrating cells and immune checkpoints such as PD-1/PD-L1 and POLD3 were positively associated with CCSPI. In conclusion, the CCSPI had prognostic value in LGG, and the model will deepen our cognition of the interaction between the CNS and immune system in different LGG subtypes.

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“…In this study, Gene Expression Profiling Interactive Analysis (GEPIA, http://gepia.cancer-pku.cn/), an online bioinformatics analysis tool (22), was used to explore the mRNA expression of NUP205 in LGG. We collected data from the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) database to verify the results of the GEPIA database (GSE12657: 13 LGG tissues vs 5 control tissues; GSE21354: 10 LGG tissues vs 4 normal brain tissues; GSE70231: 24 LGG tissues vs 6 normal brain tissues) (23)(24)(25). In the database of The Cancer Genome Atlas (TCGA, https://portal.gdc.cancer.gov/) (26), we collected RNA sequencing data with the corresponding clinical information of 503 patients with LGG and the DNA methylation sequencing data of 511 patients with LGG, which were used to explore the effects of NUP205 on the prognosis, clinicopathological characteristics, regulatory mechanism, and TIME in LGG.…”

Section: Data Collection and Tissue Preparationmentioning

confidence: 99%

Exploring the relationship between abnormally high expression of NUP205 and the clinicopathological characteristics, immune microenvironment, and prognostic value of lower-grade glioma

Liang

Zhu

et al. 2023

Front. Oncol.

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Nuclear pore complex (NPC) is a major transport pivot for nucleocytoplasmic molecule exchange. Nucleoporin 205 (NUP205)—a main component of NPC—plays a key regulatory role in tumor cell proliferation; however, few reports document its effect on the pathological progression of lower-grade glioma (LGG). Therefore, we conducted an integrated analysis using 906 samples from multiple public databases to explore the effects of NUP205 on the prognosis, clinicopathological characteristics, regulatory mechanism, and tumor immune microenvironment (TIME) formation in LGG. First, multiple methods consistently showed that the mRNA and protein expression levels of NUP205 were higher in LGG tumor tissue than in normal brain tissue. This increased expression was mainly noted in the higher WHO Grade, IDH-wild type, and 1p19q non-codeleted type. Second, various survival analysis methods showed that the highly expressed NUP205 was an independent risk indicator that led to reduced survival time of patients with LGG. Third, GSEA analysis showed that NUP205 regulated the pathological progress of LGG via the cell cycle, notch signaling pathway, and aminoacyl-tRNA biosynthesis. Ultimately, immune correlation analysis suggested that high NUP205 expression was positively correlated with the infiltration of multiple immune cells, particularly M2 macrophages, and was positively correlated with eight immune checkpoints, particularly PD-L1. Collectively, this study documented the pathogenicity of NUP205 in LGG for the first time, expanding our understanding of its molecular function. Furthermore, this study highlighted the potential value of NUP205 as a target of anti-LGG immunotherapy.

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A Risk Score Signature Consisting of Six Immune Genes Predicts Overall Survival in Patients with Lower-Grade Gliomas

Cited by 3 publications

References 44 publications

Identification of prognosis-related gene features in low-grade glioma based on ssGSEA

Identification of prognosis-related gene features in low-grade glioma based on ssGSEA

A Novel CRISPR/Cas9 Screening Potential Index for Prognostic and Immunological Prediction in Low-Grade Glioma

Exploring the relationship between abnormally high expression of NUP205 and the clinicopathological characteristics, immune microenvironment, and prognostic value of lower-grade glioma

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