A rice root-knot nematode Meloidogyne graminicola-resistant mutant rice line shows early expression of plant-defence genes

Dash, Manoranjan; Somvanshi, Vishal Singh; Budhwar, Roli; Godwin, Jeffrey; Shukla, Rohit Nandan; Rao, Uma

doi:10.1007/s00425-021-03625-0

Cited by 11 publications

(6 citation statements)

References 65 publications

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“…In particular, genes involved in the biosynthesis of secondary metabolites seemed to contain majority of common InDels and SNPs. This is interesting as a previous study regarding changes in transcriptomic profile in line-9 after nematode infection showed up-regulation of genes involved in the production of rice phytoalexins such as oryzalexins phytocassanes, momilactones and several flavonoid compounds ( Dash et al, 2021 ). It may be suggested that in addition to the effect of activation tagging, these genomic structural variants may also be contributing to altered phytoalexin and flavonoid production in the mutants.…”

Section: Discussionmentioning

confidence: 78%

Exploring Genomic Variations in Nematode-Resistant Mutant Rice Lines

Dash

Somvanshi

Godwin³

et al. 2022

Front. Plant Sci.

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Rice (Oryza sativa) production is seriously affected by the root-knot nematode Meloidogyne graminicola, which has emerged as a menace in upland and irrigated rice cultivation systems. Previously, activation tagging in rice was utilized to identify candidate gene(s) conferring resistance against M. graminicola. T-DNA insertional mutants were developed in a rice landrace (acc. JBT 36/14), and four mutant lines showed nematode resistance. Whole-genome sequencing of JBT 36/14 was done along with the four nematode resistance mutant lines to identify the structural genetic variations that might be contributing to M. graminicola resistance. Sequencing on Illumina NovaSeq 6000 platform identified 482,234 genetic variations in JBT 36/14 including 448,989 SNPs and 33,245 InDels compared to reference indica genome. In addition, 293,238–553,648 unique SNPs and 32,395–65,572 unique InDels were found in the four mutant lines compared to their JBT 36/14 background, of which 93,224 SNPs and 8,170 InDels were common between all the mutant lines. Functional annotation of genes containing these structural variations showed that the majority of them were involved in metabolism and growth. Trait analysis revealed that most of these genes were involved in morphological traits, physiological traits and stress resistance. Additionally, several families of transcription factors, such as FAR1, bHLH, and NAC, and putative susceptibility (S) genes, showed the presence of SNPs and InDels. Our results indicate that subject to further genetic validations, these structural genetic variations may be involved in conferring nematode resistance to the rice mutant lines.

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Section: Discussionmentioning

confidence: 78%

Exploring Genomic Variations in Nematode-Resistant Mutant Rice Lines

Dash

Somvanshi

Godwin³

et al. 2022

Front. Plant Sci.

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“…Interestingly, rice plants treated with AIP also show increased resistance to M. graminicola (Liu et al, 2024). Furthermore, a RKN-resistant mutant indica line showed upregulation of the diterpenoid synthesis pathway upon early recognition of M. graminicola (Dash et al, 2021). Previous studies have demonstrated diterpenoid production to be inducible by jasmonic acid (JA), a hormone that regulates anti-herbivore and anti-fungal defenses (Shimizu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Systems Biology Identifies Genetic Trade-offs In Rice Defense Against Above- and Belowground Attackers

Dunivant,

Godinez-Vidal,

Perkins

et al. 2024

Preprint

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Like other plants, wild and domesticated rice species (Oryza nivara,O. rufipogon, andO. sativa) evolve in environments with various biotic and abiotic stresses that fluctuate in intensity through space and time. Microbial pathogens and invertebrate herbivores such as plant-parasitic nematodes and caterpillars show geographical and temporal variation in activity patterns and may respond differently to certain plant defensive mechanisms. As such, plant interactions with multiple community members may result in conflicting selection pressures on genetic polymorphisms. Here, through assays with different above- and belowground herbivores, the fall armyworm (Spodoptera frugiperda) and the southern root-knot nematode (Meloidogyne incognita), respectively, and comparison with rice responses to microbial pathogens, we identify potential genetic trade-offs at theKSL8andMG1loci on chromosome 11.KSL8encodes the first committed step towards biosynthesis of either stemarane- or stemodane-type diterpenoids through the japonica (KSL8-jap) or indica (KSL8-ind) allele. Knocking outKSL8-japandCPS4, encoding an enzyme that acts upstream in diterpenoid synthesis, in japonica rice cultivars increased resistance toS. frugiperdaand decreased resistance toM. incognita. Furthermore,MG1resides in a haplotype that provided resistance toM. incognita, while alternative haplotypes are involved in mediating resistance to the rice blast fungusMagnaporthe oryzaeand other pests and pathogens. Finally,KSL8andMG1alleles are located within trans-species haplotypes and may be evolving under long-term balancing selection. Our data are consistent with a hypothesis that polymorphisms atKSL8andMG1may be maintained through complex and diffuse community interactions.

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“…Regardless of their role, oryzalexins have been shown to accumulate after exposure to fungal proteins [ 203 ] and oligosaccharides [ 204 , 205 , 206 , 207 ], as well as fungal [ 208 ] and nematode [ 209 ] infection. Abiotic factors also seem to induce the accumulation of oryzalexins, such as heavy metal ions [ 54 ], salicylic acid [ 210 ], and UV radiation [ 53 , 211 ].…”

Section: Biological Activities Of Rice Phytoalexinsmentioning

confidence: 99%

Rice Phytoalexins: Half a Century of Amazing Discoveries; Part I: Distribution, Biosynthesis, Chemical Synthesis, and Biological Activities

Valletta

Iozia

Fattorini

et al. 2023

Plants

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Cultivated rice is a staple food for more than half of the world’s population, providing approximately 20% of the world’s food energy needs. A broad spectrum of pathogenic microorganisms causes rice diseases leading to huge yield losses worldwide. Wild and cultivated rice species are known to possess a wide variety of antimicrobial secondary metabolites, known as phytoalexins, which are part of their active defense mechanisms. These compounds are biosynthesized transiently by rice in response to pathogens and certain abiotic stresses. Rice phytoalexins have been intensively studied for over half a century, both for their biological role and their potential application in agronomic and pharmaceutical fields. In recent decades, the growing interest of the research community, combined with advances in chemical, biological, and biomolecular investigation methods, has led to a notable acceleration in the growth of knowledge on rice phytoalexins. This review provides an overview of the knowledge gained in recent decades on the diversity, distribution, biosynthesis, chemical synthesis, and bioactivity of rice phytoalexins, with particular attention to the most recent advances in this research field.

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A rice root-knot nematode Meloidogyne graminicola-resistant mutant rice line shows early expression of plant-defence genes

Cited by 11 publications

References 65 publications

Exploring Genomic Variations in Nematode-Resistant Mutant Rice Lines

Exploring Genomic Variations in Nematode-Resistant Mutant Rice Lines

Evolutionary Systems Biology Identifies Genetic Trade-offs In Rice Defense Against Above- and Belowground Attackers

Rice Phytoalexins: Half a Century of Amazing Discoveries; Part I: Distribution, Biosynthesis, Chemical Synthesis, and Biological Activities

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