A Rice Glutamyl-tRNA Synthetase Modulates Early Anther Cell Division and Patterning

Yang, Xiujuan; Li, Gang; Tian, Yaxi; Yu, Shanlin; Liang, Wanqi; Zhang, Dabing

doi:10.1104/pp.18.00110

Cited by 32 publications

(33 citation statements)

References 59 publications

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“…In rice, ROS level is extremely low during anther cell specification stages, which is less than 200 pmol mg − 1 , and the ROS level increases twice at stage 4 to stage 5 (Yang et al, 2018). 3,3′-diaminobenzidine (DAB) staining indicates the accumulation of H 2 O 2 in middle layer and endothecium from stage 5 to stage 9 and decrease of ROS at stage 10 and stage 11 (Fig.…”

Section: Dynamics Of Redox Status During Rice Anther Developmentmentioning

confidence: 99%

Molecular Control of Redox Homoeostasis in Specifying the Cell Identity of Tapetal and Microsporocyte Cells in Rice

Zhang

2019

Rice

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In flowering plants, male reproduction occurs within the male organ anther with a series of complex biological events including de novo specification of germinal cells and somatic cells, male meiosis, and pollen development and maturation. Particularly, unlike other tissue, anther lacks a meristem, therefore, both germinal and somatic cell types are derived from floral stem cells within anther lobes. Here, we review the molecular mechanism specifying the identity of somatic cells and reproductive microsporocytes by redox homoeostasis during rice anther development. Factors such as glutaredoxins (GRXs), TGA transcription factors, receptor-like protein kinase signaling pathway, and glutamyl-tRNA synthetase maintaining the redox status are discussed. We also conceive the conserved and divergent aspect of cell identity specification of anther cells in plants via changing redox status.

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Section: Dynamics Of Redox Status During Rice Anther Developmentmentioning

confidence: 99%

Molecular Control of Redox Homoeostasis in Specifying the Cell Identity of Tapetal and Microsporocyte Cells in Rice

Zhang

2019

Rice

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“…Treatment of wheat with mycotoxin deoxynivalenol activates the expression of wheat methionyl‐tRNA synthetase which participates in plant defense and detoxification conferring resistance to mycotoxins and mycotoxin‐producing fungal pathogens . Rice glutamyl‐tRNA synthetase (GluRS) modulates male organ development by affecting metabolic homeostasis and redox status . However, exact molecular mechanisms of noncanonical action of these plant aaRSs are not yet deciphered.…”

Section: Introductionmentioning

confidence: 99%

“…There is only one report on plant aaRS protein complex. Protein OsARC interacts with rice GluRS enhancing its aminoacylation activity .…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis seryl‐tRNA synthetase: the first crystal structure and novel protein interactor of plant aminoacyl‐tRNA synthetase

et al. 2019

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The rules of the genetic code are established by aminoacyl‐tRNA synthetases (aaRSs) enzymes, which covalently link tRNA with the cognate amino acid. Many aaRSs are involved in diverse cellular processes beyond translation, acting alone, or in complex with other proteins. However, studies of aaRS noncanonical assembly and functions in plants are scarce, as are structural studies of plant aaRSs. Here, we have solved the crystal structure of Arabidopsis thaliana cytosolic seryl‐tRNA synthetase (SerRS), which is the first crystallographic structure of a plant aaRS. Arabidopsis SerRS displays structural features typical of canonical SerRSs, except for a unique intrasubunit disulfide bridge. In a yeast two‐hybrid screen, we identified BEN1, a protein involved in the metabolism of plant brassinosteroid hormones, as a protein interactor of Arabidopsis SerRS. The SerRS:BEN1 complex is one of the first protein complexes of plant aaRSs discovered so far, and is a rare example of an aaRS interacting with an enzyme involved in primary or secondary metabolism. To pinpoint regions responsible for this interaction, we created truncated variants of SerRS and BEN1, and identified that the interaction interface involves the SerRS globular catalytic domain and the N‐terminal extension of BEN1 protein. BEN1 does not have a strong impact on SerRS aminoacylation activity, indicating that the primary function of the complex is not the modification of SerRS canonical activity. Perhaps SerRS performs as yet unknown noncanonical functions mediated by BEN1. These findings indicate that – via SerRS and BEN1 – a link exists between the protein translation and steroid metabolic pathways of the plant cell. Database Structural data are available in the PDB under the accession number PDB ID http://www.rcsb.org/pdb/search/structidSearch.do?structureId=6GIR.

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“…The expression of methionyl-tRNA synthetase (MetRS) gene of wheat was upregulated by a mycotoxin deoxynivalenol treatment and conferred resistance to this mycotoxin [ 11 ]. A number of characterized aaRS mutants impair particular developmental processes, but do not impair global protein synthesis, which is generally lethal [ 12 , 13 , 14 , 15 , 16 , 17 ]. These observations suggest that aaRSs in plants may have specific functions other than tRNA aminoacylation.…”

Section: Introductionmentioning

confidence: 99%

Plant-Specific Domains and Fragmented Sequences Imply Non-Canonical Functions in Plant Aminoacyl-tRNA Synthetases

Saga

Kawashima

Sakai

et al. 2020

Genes

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Aminoacyl-tRNA synthetases (aaRSs) play essential roles in protein translation. In addition, numerous aaRSs (mostly in vertebrates) have also been discovered to possess a range of non-canonical functions. Very few studies have been conducted to elucidate or characterize non-canonical functions of plant aaRSs. A genome-wide search for aaRS genes in Arabidopsis thaliana revealed a total of 59 aaRS genes. Among them, asparaginyl-tRNA synthetase (AsnRS) was found to possess a WHEP domain inserted into the catalytic domain in a plant-specific manner. This insertion was observed only in the cytosolic isoform. In addition, a long stretch of sequence that exhibited weak homology with histidine ammonia lyase (HAL) was found at the N-terminus of histidyl-tRNA synthetase (HisRS). This HAL-like domain has only been seen in plant HisRS, and only in cytosolic isoforms. Additionally, a number of genes lacking minor or major portions of the full-length aaRS sequence were found. These genes encode 14 aaRS fragments that lack key active site sequences and are likely catalytically null. These identified genes that encode plant-specific additional domains or aaRS fragment sequences are candidates for aaRSs possessing non-canonical functions.

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A Rice Glutamyl-tRNA Synthetase Modulates Early Anther Cell Division and Patterning

Cited by 32 publications

References 59 publications

Molecular Control of Redox Homoeostasis in Specifying the Cell Identity of Tapetal and Microsporocyte Cells in Rice

Molecular Control of Redox Homoeostasis in Specifying the Cell Identity of Tapetal and Microsporocyte Cells in Rice

Arabidopsis seryl‐tRNA synthetase: the first crystal structure and novel protein interactor of plant aminoacyl‐tRNA synthetase

Plant-Specific Domains and Fragmented Sequences Imply Non-Canonical Functions in Plant Aminoacyl-tRNA Synthetases

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