A rice gene that confers broad-spectrum resistance to β-triketone herbicides

Maeda, Hideo; Murata, Keiko; Sakuma, Nozomi; Takei, Satomi; Yamazaki, Akihiko; Karim, Md. Rezaul; Kawata, Manabu; Hirose, Sakiko; Kawagishi-Kobayashi, Makiko; Taniguchi, Yojiro; Suzuki, Satoru; Sekino, Keisuke; Ohshima, Masahiro; Katô, Hiroshi; Yoshida, Hitoshi; Tozawa, Yuzuru

doi:10.1126/science.aax0379

Cited by 69 publications

(98 citation statements)

References 32 publications

(19 reference statements)

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“…These P450 inhibitor studies suggest that multiple metabolic genes/enzymes (including P450s) may be involved in weed resistance to HPPD‐inhibiting herbicides. A recent study has identified that a gene encoding an Fe(II)/2‐oxoglutarate‐dependent oxygenase endows resistance to HPPD‐inhibiting herbicides in rice . However, genes conferring metabolic resistance to HPPD‐inhibiting herbicides have not been identified in weeds.…”

Section: Discussionmentioning

confidence: 99%

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Evolution of resistance to HPPD‐inhibiting herbicides in a wild radish population via enhanced herbicide metabolism

Han

et al. 2020

Pest Management Science

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BACKGROUND Relatively new herbicides that target 4‐hydroxyphenylpyruvate dioxygenase (HPPD) are now available for use on the world's great grain crops (rice, wheat, corn and soybean) and for other uses. With widespread and persistent use of HPPD‐inhibiting herbicides, the evolution of HPPD‐inhibiting herbicide resistant weeds is inevitable. Currently, resistance to HPPD‐inhibiting herbicides is known in two weed species, waterhemp and Palmer amaranth. Here, we report a HPPD‐inhibiting herbicide resistant wild radish population from the Western Australia grain belt. This population was not selected with HPPD‐inhibiting herbicides, rather it evolved resistance to earlier used herbicides with different modes of action and exhibits cross‐resistance to HPPD‐inhibiting herbicides. RESULTS Dose–response experiments showed the resistant (R) population exhibits 4 to 6.5‐fold resistance to the HPPD‐inhibiting herbicides mesotrione, tembotrione and isoxaflutole, compared to the susceptible (S) population. This resistance is not target‐site based as cloning of full coding sequences of the HPPD genes from S and R plants did not reveal resistance‐endowing single nucleotide polymorphisms. The HPPD gene expression levels are similar in S and R plants. In addition, no differences in [14C]‐mesotrione uptake and translocation were observed in the S and R plants. However, the time required for R plants to metabolise 50% [14C]‐mesotrione is 7.7‐fold faster than for the S plants. CONCLUSION We confirm resistance to HPPD‐inhibiting herbicides exists in a population of the economically damaging global weed wild radish. The resistance in this population is due to a non‐target‐site based enhanced rate of herbicide metabolism. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

“…A recent study has identified that a gene encoding an Fe(II)/2-oxoglutarate-dependent oxygenase endows resistance to HPPD-inhibiting herbicides in rice. 37 However, genes conferring metabolic resistance to HPPD-inhibiting herbicides have not been identified in weeds.…”

Section: Discussionmentioning

confidence: 99%

Evolution of resistance to HPPD‐inhibiting herbicides in a wild radish population via enhanced herbicide metabolism

Han

et al. 2020

Pest Management Science

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“…Benzobicyclon (BBC) is a novel paddy-bleaching β-triketone herbicide (bTH) (Komatsubara et al, 2009). It can be applied from pre-emergence to early post-emergence in paddy elds, showing broad-spectrum resistance against annual grasses, sedges, and broadleaf weeds but being harmless to TPR and DSR (Bi et al, 2018;Komatsubara et al, 2009;Maeda et al, 2019). bTHs mainly target 4-hydroxyphenylpyruvate dioxygenase (HPPD), which is a key step in the biosynthesis of plastoquinone and tocopherol (Kraehmer et al, 2014;Moran, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…As an herbicide that inhibits HPPD, BBC is different from herbicides that inhibit acetolactate synthetase (ALS) and acetyl-CoA carboxylase (ACCase), which are widely used in paddy elds (Jabusch and Tjeerdema, 2005;Kumar and Ladha, 2011;Mahajan et al, 2013), so it can be used as a rotation herbicide in these elds. The number of weed species with resistance to herbicides that inhibit HPPD is minimal (Gould et al, 2018), and BBC shows e cacy against paddy weeds resistant to other types of herbicide, including sulfonylureas (Maeda et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the rice gene HIS1 (HPPD INHIBITOR SENSITIVE 1), which confers resistance to BBC and other bTHs in japonica varieties, was identi ed. HIS1 encodes a Fe(II)/2-oxoglutarate-dependent oxygenase that detoxi es bTHs by catalyzing their hydroxylation (Maeda et al, 2019). Sequence alignment revealed that his1 in BBC-sensitive varieties harbors a 28-bp deletion in the third exon, which leads to a nonsense mutation (Maeda et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Exploring natural allelic variations of the β-triketone herbicide resistance gene HIS1 for application in indica rice and particularly in two-line hybrid rice

Zhang

Yuan

et al. 2020

Preprint

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Background Benzobicyclon (BBC) is a β-triketone herbicide (bTH) used in rice paddy fields. It has the advantages of high efficiency, low toxicity, high crop safety, and good environmental compatibility, and shows efficacy against paddy weeds resistant to other types of herbicides. However, because BBC is not safe for some important indica rice varieties, it is currently only registered and applied in japonica rice areas. Results In this study, by analyzing haplotypes of the bTHs broad-spectrum resistance gene HIS1 and phenotypes for BBC in 493 major indica rice accessions in China, we identified a novel non-functional allelic variant of HIS1 besides the previously reported 28-bp deletion. Through detection with markers specific to the two non-functional mutations, it was clear that 25.4% of indica conventional varieties, 59.9% of restorers and 15.9% of sterile lines were susceptible to BBC. In addition, due to natural allelic variations of the HIS1 gene in sterile and restorer lines, some two-line hybrid sterile lines were sensitive to bTHs, and the corresponding restorers were resistant. We showed the potential of effectively using bTHs to solve the problem of two-line hybrid rice seed purity due to self-crossing of sterile lines during hybrid rice seed production. Finally, allelic variations of the HIS1 gene may also play an important role in the mechanized seed production of hybrid rice. Conclusions Our findings provide guidance for the application of BBC in indica rice areas, and provide a non-transgenic approach to solve the problem of seed purity of two-line hybrid rice.

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Rice tolerance to multiple applications of florpyrauxifen‐benzyl alone and followed by benzobicyclon

Beesinger

Norsworthy

Butts

et al. 2022

Crop Forage & Turfgrass Mgmt

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Varying levels of sensitivity to florpyrauxifen‐benzyl and benzobicyclon applications among rice (Oryza sativa L.) cultivars have been observed. The safe use of florpyrauxifen‐benzyl and benzobicyclon in the same herbicide program could provide broad‐spectrum weed control and delay resistance in weed species. Two experiments were conducted in 2019 and 2020 near Stuttgart, AR, to test cultivars’ sensitivity to multiple rates of florpyrauxifen‐benzyl and applications of florpyrauxifen‐benzyl followed by benzobicyclon. The first experiment was designed to determine the sensitivity of the rice cultivars ‘XP753’, ‘CL XL745’, ‘Gemini 214CL’, ‘Titan’, and ‘Diamond’ to applications of florpyrauxifen‐benzyl at 0.03 and 0.06 lb acid equivalent (a.e.) acre–1 and sequential applications of 0.03 lb a.e. acre–1. The second experiment determined the effect of benzobicyclon applied post‐flood on rice injury following an application of florpyrauxifen‐benzyl on the same cultivars used in the previous experiment. A rough rice yield loss of 17% was observed for sequential applications of florpyrauxifen‐benzyl at 0.03 lb a.e. acre–1 to XP753. Single applications of florpyrauxifen‐benzyl at 0.06 lb a.e. acre–1 caused a 45% groundcover reduction in XP753, whereas sequential applications of 0.03 lb a.e. acre–1 reduced groundcover on all hybrid cultivars tested. Applications of benzobicyclon alone never injured rice by more than 8%, and applying benzobicyclon after florpyrauxifen‐benzyl did not increase the observed injury over florpyrauxifen‐benzyl alone. Single applications of florpyrauxifen‐benzyl followed by benzobicyclon can be used in an herbicide program to control weeds without the risk of greater injury than is typically caused by florpyrauxifen‐benzyl alone.

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A rice gene that confers broad-spectrum resistance to β-triketone herbicides

Cited by 69 publications

References 32 publications

Evolution of resistance to HPPD‐inhibiting herbicides in a wild radish population via enhanced herbicide metabolism

Evolution of resistance to HPPD‐inhibiting herbicides in a wild radish population via enhanced herbicide metabolism

Exploring natural allelic variations of the β-triketone herbicide resistance gene HIS1 for application in indica rice and particularly in two-line hybrid rice

Rice tolerance to multiple applications of florpyrauxifen‐benzyl alone and followed by benzobicyclon

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