A Rice Cytochrome P450<i>OsCYP84A</i>That May Interact with the UV Tolerance Pathway

Sasaki, Toyokazu; Akutsu, Haruko; Shimada, Hiroaki; Miura, Shogo

doi:10.1271/bbb.90942

Cited by 11 publications

(5 citation statements)

References 34 publications

(33 reference statements)

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“…In the phylogenetic tree of HORVU2Hr1G092360 and CYP genes with clear function reported, HORVU2Hr1G092360 showed a high bootstrap value to the CYP84 gene that function as ferulate-5-hydroxylase ( F5H ) from Arabidopsis thaliana and Oryza sativa ( Figure 1E ). F5H is a key gene of lignin synthesis pathway in plants, and genetic evidence suggests that CYP84 gene in rice is involved in ultraviolet tolerance ( Ruegger et al., 1999 ; SASAKI et al., 2010 ).…”

Section: Resultsmentioning

confidence: 99%

Genetic architecture of inducible and constitutive metabolic profile related to drought resistance in qingke (Tibetan hulless barley)

Yu¹,

Wei²,

Yuan³

et al. 2022

Front. Plant Sci.

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Qingke (Tibetan hulless barley, Hordeum vulgare L. var. nudum) is the primary food crop on the Tibet Plateau, the long-term drought and other harsh environments makes qingke an important resource for the study of abiotic resistance. Here, we evaluated the drought sensitivity of 246 qingke varieties. Genome-wide association studies (GWAS) found that root-specific expressed gene CYP84 may be involved in the regulation of drought resistance. Based on widely targeted metabolic profiling, we identified 2,769 metabolites in qingke leaves, of which 302 were significantly changed in response to drought stress, including 4-aminobutyric acid (GABA), proline, sucrose and raffinose. Unexpectedly, these drought-induced metabolites changed more violently in drought-sensitive qingkes, while the constitutive metabolites that had little response to drought stress, such as C-glycosylflavonoids and some amino acids, accumulated excessively in drought-resistant qingkes. Combined with metabolite-based genome-wide association study (mGWAS), a total of 1,006 metabolites under optimal condition and 1,031 metabolites under mild drought stress had significant associated loci. As a marker metabolite induced by drought stress, raffinose was significantly associated with two conservatively adjacent α-galactosidase genes, qRT-PCR suggests that these two genes may jointly regulate the raffinose content in qingke. Besides, as constituent metabolites with stable differences between drought-sensitive and drought-resistant qingkes, a class of C-glycosylflavonoids are simultaneously regulated by a UDP-glucosyltransferase gene. Overall, we performed GWAS for sensitivity and widely targeted metabolites during drought stress in qingke for the first time, which provides new insights into the response mechanism of plant drought stress and drought resistance breeding.

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Section: Resultsmentioning

confidence: 99%

Genetic architecture of inducible and constitutive metabolic profile related to drought resistance in qingke (Tibetan hulless barley)

Yu¹,

Wei²,

Yuan³

et al. 2022

Front. Plant Sci.

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“…If the tolerance limit is exceeded, plants may expend a significant amount of energy to resist external stresses, resulting in poor growth and development. Under normal growth conditions, OsCYP84A was widely expressed; however, under UV-B radiation, it was repressed, resulting in marked growth retardation and obvious signs of plant damage [ 39 ]. As a result of high-intensity UV-B radiation treatment, OsGLP1 knockout rice mutant plants showed an increased leaf angle under solar radiation (including UV-B).…”

Section: Discussionmentioning

confidence: 99%

Effects and Mechanism of Enhanced UV-B Radiation on the Flag Leaf Angle of Rice

Ling

Wang²,

Li³

et al. 2022

IJMS

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Leaf angle is an influential agricultural trait that influences rice (Oryza sativa L.) plant type and yield, which results from the leaf bending from the vertical axis to the abaxial axis. UV-B radiation affects plant morphology, but the effects of varying UV-B intensities on rice flag leaves and the underlying molecular, cellular, and physiological mechanisms remain unknown. This experiment aims to examine the effect of natural light and field-enhanced UV-B radiation (2.5, 5.0, 7.5 kJ·m−2) on the leaf angle of the traditional rice variety Baijiaolaojing on Yuanyang terraces. In comparison with natural light, the content of brassinolide and gibberellin in rice flag leaves increased by 29.94% and 60.1%, respectively. The auxin content decreased by 17.3%. Compared with the natural light treatment, the cellulose content in the pulvini was reduced by 13.8% and hemicellulose content by 25.7% under 7.5 kJ·m−2 radiation intensity. The thick-walled cell area and vascular bundle area of the leaf pulvini decreased with increasing radiation intensity, and the growth of mechanical tissue in the rice leaf pulvini was inhibited. The flag leaf angle of rice was greatest at 7.5 kJ·m−2 radiation intensity, with an increase of 50.2%. There are two pathways by which the angle of rice flag leaves is controlled under high-intensity UV-B radiation. The leaf angle regulation genes OsBUL1, OsGSR1, and OsARF19 control hormone levels, whereas the ILA1 gene controls fiber levels. Therefore, as cellulose, hemicellulose, sclerenchyma, and vascular bundles weaken the mechanical support of the pulvini, the angle of the flag leaf increases.

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“…3). Among those signi cantly more highly expressed in the root have been associated with fertility reduction (CYP78A), UV stress tolerance (CYP84A), gibberellin metabolism (CYP714A) and jasmonic acid metabolism (CYP74A) [44][45][46][47]. Those signi cantly more highly expressed in the leaf include those previously associated with catalysing successive oxidation steps of the plant hormone jasmonoyl-isoleucine for catabolic turnover (CYP94), expression of ABA 8'-hydroxylase and affects ABA levels to control seed dormancy (CYP707A), hydroxylation of carotenoids (CYP97), biosynthesis of castasterone in the brassinosteroid biosynthetic pathway (CYP85A) and glucosinolate metabolism (CYP83) [37,43,[48][49][50].…”

Section: Discussionmentioning

confidence: 99%

The CYP450 Multigene Family of Fontainea and Insights Into Diterpenoid Synthesis

Mitu

Ogbourne

Klein

et al. 2021

Preprint

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Background: Cytochrome P450s (CYP450s) are enzymes that play critical roles in the biosynthesis of physiologically important compounds across all organisms. Although they have been characterised in a large number of plant species, no information relating to these enzymes are available from the genus Fontainea (family Euphorbiaceae). Fontainea is significant as the genus includes species that produce medicinally significant epoxy-tigliane natural products, one of which has been approved as an anti-cancer therapeutic. Results: A metabolome analysis showed that Fontainea species possess a chemical profile different from various other plant species. The diversity and expression profiles of Fontainea CYP450s were investigated from leaf and root tissue. A total of 103 and 123 full-length CYP450 genes in Fontainea picrosperma and Fontainea venosa, respectively (and a further 127/125 partial-length) that were phylogenetically classified into clans, families and subfamilies. The majority of CYP450 identified are most active within root tissue (66.2% F. picrosperma, 65.0% F. venosa). Fontainea CYP450 associated with diterpenoid biosynthesis were classified into 2 subfamilies (CYP71D and CYP726A), of which CYP726A1, CYP726A2 and CYP71D1 appear to be exclusive to Fontainea species, and significantly more highly expressed in root tissue compared to leaf tissue. Conclusion: This study presents a comprehensive overview of the CYP450 gene family in Fontainea that may provide important insights into the biosynthesis of the medicinally significant epoxy-tigliane diterpenes found within the genus.

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A Rice Cytochrome P450OsCYP84AThat May Interact with the UV Tolerance Pathway

Cited by 11 publications

References 34 publications

Genetic architecture of inducible and constitutive metabolic profile related to drought resistance in qingke (Tibetan hulless barley)

Genetic architecture of inducible and constitutive metabolic profile related to drought resistance in qingke (Tibetan hulless barley)

Effects and Mechanism of Enhanced UV-B Radiation on the Flag Leaf Angle of Rice

The CYP450 Multigene Family of Fontainea and Insights Into Diterpenoid Synthesis

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