A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility

Wei, Shuya; Hu, Wei; Deng, Xianwen; Zhang, Yingying; Liu, Xiaodong; Zhao, Xuelin; Luo, Qingchen; Zheng-yi, Jin; Yin, Lei; Zhou, Shiyi; Sun, Tao; Wang, Lianzhe; Yang, Guangxiao; He, Guoqing

doi:10.1186/1471-2229-14-133

Cited by 185 publications

(141 citation statements)

References 69 publications

(87 reference statements)

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“…In addition, the expression data used in this study also show some discrepancies with other published studies. For example in rice, OsCPK9 and OsCPK21 have been reported to be induced by salt stress and ABA 22, 48, which is not observed in Fig. 4F.…”

Section: Resultsmentioning

confidence: 78%

The calcium‐dependent protein kinase (CDPK) and CDPK‐related kinase gene families in Hevea brasiliensis—comparison with five other plant species in structure, evolution, and expression

et al. 2016

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Calcium‐dependent protein kinases (CDPKs or CPKs) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. Although the CDPK gene family has been characterized in several model plants, little is known about this gene family in Hevea brasiliensis (the Para rubber tree). Here, we characterize the entire H. brasiliensis CDPK and CDPK‐related kinase (CRK) gene families comprising 30 CDPK genes (HbCPK1 to 30) and nine CRK genes (HbCRK1 to 9). Structure and phylogeny analyses of these CDPK and CRK genes demonstrate evolutionary conservation in these gene families across H. brasiliensis and other plant species. The expression of HbCPK and HbCRK genes was investigated via Solexa sequencing in a range of experimental conditions (different tissues, phases of leaf development, ethylene treatment, and various abiotic stresses). The results suggest that HbCPK and HbCRK genes are important components in growth, development, and stress responses of H. brasiliensis. Parallel studies on the CDPK and CRK gene families were also extended to five other plant species (Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, Manihot esculenta, and Ricinus communis). The CDPK and CRK genes from different plant species that exhibit similar expression patterns tend to cluster together, suggesting a coevolution of gene structure and expression behavior in higher plants. The results serve as a foundation to further functional studies of these gene families in H. brasiliensis as well as in the whole plant kingdom.

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Section: Resultsmentioning

confidence: 78%

The calcium‐dependent protein kinase (CDPK) and CDPK‐related kinase gene families in Hevea brasiliensis—comparison with five other plant species in structure, evolution, and expression

et al. 2016

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“…(Wei et al, 2014). Taken together these results suggest that the genes in this cluster contribute to drought tolerance by regulating osmotic adjustment and ROS scavenging processes and can also be putative candidates for increasing yield under drought.…”

Section: Examples Of Drought Transcriptional Modulesmentioning

confidence: 65%

RECoN: Rice Environment Coexpression Network for Systems Level Analysis of Abiotic-Stress Response

Krishnan

Gupta

Ambavaram

et al. 2017

Preprint

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Author ContributionsAK, designed the network, conducted statistical analysis, drafted the manuscript; CG, contributed text, updated figures, and created the webserver; MA, conducted rice drought and gene expression experiments; AP, designed experiments and coordinated research; all authors contributed to writing the manuscript. AbstractTranscriptional profiling is a prevalent and powerful approach for capturing the response of crop plants to environmental stresses, e.g. response of rice to drought. However, functionally . CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/166694 doi: bioRxiv preprint first posted online 2 interpreting the resulting genome-wide gene expression changes is severely hampered by the large gaps in our genomic knowledge about which genes work together in cellular pathways/processes in rice. Here, we present a new web resource -RECoN -that relies on a network-based approach to go beyond currently limited annotations in delineating functional and regulatory perturbations in new rice stress transcriptome datasets generated by a researcher. To build RECoN, we first enumerated 1,744 stress-specific gene modules covering 28,421 rice genes (>72% of the genes in the genome). Each module contains a group of genes tightly coexpressed across a large number of environmental conditions and, thus, is likely to be functionally coherent. When a user provides a new differential expression profile, RECoN identifies modules substantially perturbed in their experiment and further suggests deregulated functional and regulatory mechanisms based on the enrichment of current annotations within the predefined modules. We demonstrate the utility of this resource by analyzing new drought transcriptomes of rice in three developmental stages, which revealed large-scale insights into the cellular processes and regulatory mechanisms involved in common and stage-specific drought responses. RECoN enables biologists to functionally explore new data from all abiotic stresses on a genome-scale and to uncover gene candidates, including those that are currently functionally uncharacterized, for engineering stress tolerance.

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“…However, the abundance of CDPKs increased in the drought-tolerant genotype of Z. mays [41]. It has been reported that overexpression of the CDPK gene can enhance drought tolerance in transgenic Arabidopsis [79,80] and O. sativa [81]. These reports imply the important role of CDPKs in plant stress tolerance.…”

Section: Drought Sensing and Signalingmentioning

confidence: 99%

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Wang

Cai

et al. 2016

IJMS

210

143

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Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different plants. Although drought-responsive proteins exhibit various patterns depending on plant species, genotypes and stress intensity, proteomic analyses show that dominant changes occurred in sensing and signal transduction, reactive oxygen species scavenging, osmotic regulation, gene expression, protein synthesis/turnover, cell structure modulation, as well as carbohydrate and energy metabolism. In combination with physiological and molecular results, proteomic studies in leaves have helped to discover some potential proteins and/or metabolic pathways for drought tolerance. These findings provide new clues for understanding the molecular basis of plant drought tolerance.

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A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility

Cited by 185 publications

References 69 publications

The calcium‐dependent protein kinase (CDPK) and CDPK‐related kinase gene families in Hevea brasiliensis—comparison with five other plant species in structure, evolution, and expression

The calcium‐dependent protein kinase (CDPK) and CDPK‐related kinase gene families in Hevea brasiliensis—comparison with five other plant species in structure, evolution, and expression

RECoN: Rice Environment Coexpression Network for Systems Level Analysis of Abiotic-Stress Response

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

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