A ribosome-inactivating protein (curcin 2) induced from Jatropha curcas can reduce viral and fungal infection in transgenic tobacco

Huang, Mingxing; Hou, Pei; Qin, Wei; Xu, Ying; Chen, Fang

doi:10.1007/s10725-007-9234-7

Cited by 43 publications

(36 citation statements)

References 43 publications

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“…As a multi-purpose tree, it has a long history in reclaiming land and making feedstuff, soap, cosmetics, pesticide, anti-cancerous, antiviral and anti-fungal medicine, and other traditional Chinese medicine (STAUBMANN et al, 1999;OPENSHAW, 2000;LIN et al, 2004;MAMPANE et al, 2006;HUANG et al, 2008). Recently, J. curcas has drawn increased attention for its high seed oil content (50~60 %), which exceeds seed oil content of other biodiesel plants such as cole and soybean.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

Pasapula

et al. 2009

Silvae Genetica

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An efficient system for Agrobacterium tumefaciensmediated transformation of Jatropha curcas was developed in this study. Several factors affecting the transformation efficiency were optimized, including the explant type, preculture and coculture periods, usage of acetosyringone and density of A. tumefaciens. Compared with other explants, 2-day precultured and cocultured hypocotyl explants showed a significant GUS transient expression efficiency (67.7 %). Moreover, adding AS showed a remarkable increase in transformation efficiency. After infecting with A. tumefaciens, hypocotyl explants were subjected to expansion and proliferation on MS medium with 1.0 mg · l -1 IBA and 0.5 mg · l -1 BA. Transformants were demonstrated by the GUS assay and PCR analysis. Rate of hypocotyl explants forming resistant calli reached 67.08 %. Furthermore, in the transformed calli development, morphologic changes (calli superficial development and internal structure) were observed by SEM and LM. The present study has provided a fundamental information and research approach for the future study of inducing new traits to J. curcas and genetic modification.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

Pasapula

et al. 2009

Silvae Genetica

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“…Some studies report that RIPs may be involved in the plant defense response [52–54]. Although RIPs have been classified into three groups, based on their structure and functions, type I RIPs are most important in J. curcas.…”

Section: Functional Genomics Analysis Of Economically Important Tramentioning

confidence: 99%

Jatropha curcas, a biofuel crop: Functional genomics for understanding metabolic pathways and genetic improvement

Maghuly

Laimer

2013

Biotechnology Journal

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Jatropha curcas is currently attracting much attention as an oilseed crop for biofuel, as Jatropha can grow under climate and soil conditions that are unsuitable for food production. However, little is known about Jatropha, and there are a number of challenges to be overcome. In fact, Jatropha has not really been domesticated; most of the Jatropha accessions are toxic, which renders the seedcake unsuitable for use as animal feed. The seeds of Jatropha contain high levels of polyunsaturated fatty acids, which negatively impact the biofuel quality. Fruiting of Jatropha is fairly continuous, thus increasing costs of harvesting. Therefore, before starting any improvement program using conventional or molecular breeding techniques, understanding gene function and the genome scale of Jatropha are prerequisites. This review presents currently available and relevant information on the latest technologies (genomics, transcriptomics, proteomics and metabolomics) to decipher important metabolic pathways within Jatropha, such as oil and toxin synthesis. Further, it discusses future directions for biotechnological approaches in Jatropha breeding and improvement.

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“…Curcin, a RIP isolated from seeds of Jatropha curcas showed anti-tumor activity (Lin et al 2003). Subsequently, another RIP designated as curcin 2 with antiviral and antifungal property was transformed into tobacco (Huang et al 2008).…”

Section: Ribosome-inactivating Proteinsmentioning

confidence: 99%

Pathogenesis-related genes and proteins in forest tree species

Veluthakkal

Dasgupta

2010

Trees

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Trees occupy more than 30% of the land biosphere. They are important from both ecological and environmental standpoints and provide some of the most valuable commodities in the world economy. The perennial nature and size of trees are the critical determinants of their survival in response to biotic and abiotic stresses. The identification of the defense pathways at biochemical and genetic levels in tree pathosystems are beginning to be addressed. The basic physiological and biochemical mechanisms in woody perennials in response to pathogen is homologous to the model annual crop like Arabidopsis, but their secondary metabolic processes and ecological survival strategies are likely to be divergent from their annual counterparts. The limited domestication in tree species makes its molecular mechanisms less comparable to the highly pedigreed crop species. Recent reports have highlighted that the possible difference in genetic programs responding to invasive pathogens between annuals and perennials could be the spatial and temporal pattern of gene regulation. Several reviews on pathogen defense with reference to crop species are available, while similar reports from the tree species are limited to few commercially important species like Populus, Pinus, Picea, Eucalyptus, Castanea, and Pseudotsuga. This paper reviews the present status of pathogenesis-related genes and proteins from tree species with emphasis on the resistant genes and the proteins induced during systemic acquired resistance and highlights the ecological and evolutionary significance of defense-related genes from tree species.

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A ribosome-inactivating protein (curcin 2) induced from Jatropha curcas can reduce viral and fungal infection in transgenic tobacco

Cited by 43 publications

References 43 publications

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

Jatropha curcas, a biofuel crop: Functional genomics for understanding metabolic pathways and genetic improvement

Pathogenesis-related genes and proteins in forest tree species

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