A Ribonuclease Isolated from Wild Ganoderma Lucidum Suppressed Autophagy and Triggered Apoptosis in Colorectal Cancer Cells

Dan, Xiuli; Liu, Wenlong; Wong, Jack Ho; Ng, Tzi Bun

doi:10.3389/fphar.2016.00217

Cited by 28 publications

(16 citation statements)

References 47 publications

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“…G. lucidum ribonuclease was detected to suppress cell autophagy in colorectal cancer cells. 34 On the contrary, the methanolic extract of G. lucidum induces autophagy of gastric adenocarcinoma cells. 34 In this study, we found that BSGLWE induced autophagosome formation by the results of TEM, Western blot and immunofluorescence.…”

Section: Discussionmentioning

confidence: 99%

“…34 On the contrary, the methanolic extract of G. lucidum induces autophagy of gastric adenocarcinoma cells. 34 In this study, we found that BSGLWE induced autophagosome formation by the results of TEM, Western blot and immunofluorescence. The formation of autophagosome is only one aspect of assessing autophagy activity, and degradation of autophagy substrates needs to be detected.…”

Section: Discussionmentioning

confidence: 99%

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Water Extract of Sporoderm-Broken Spores of Ganoderma lucidum Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux

Zhang¹,

Zhong²,

Meng³

et al. 2019

OTT

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Osteosarcoma (OS) is a malignant bone tumor with easy metastasis and poor prognosis. Ganoderma lucidum (G. lucidum), a traditional Chinese medicine, was reported playing a critical role in suppressing multiple tumor progress. So we wanted to investigate the effects and molecular mechanisms of water extract of sporoderm-broken spores of G. lucidum (BSGLWE) on osteosarcoma. Methods: In vitro, the effects on cell proliferation of BSGLWE in osteosarcoma cells were detected by CCK-8, colony formation assay and flow cytometry; migration ability of osteosarcoma cells was evaluated by cell scratch and transwell assays. Cell apoptosis and autophagy were tested by transmission electron microscopy (TEM). Potential signaling pathways were detected by Western blotting and immunofluorescence. In xenograft orthotopic model, the luminescence intensity measured by an in vivo bioluminescence imaging system, and the expression of related proteins in tumor cells were assessed by IHC analysis. Results: BSGLWE suppressed the proliferation and migration of osteosarcoma cells in a dose-dependent manner, and osteosarcoma cell cycle progression at the G2/M phase was arrested by the BSGLWE. In addition, increased apoptosis-related protein expression meant BSFLWE induced caspase-dependent apoptosis of osteosarcoma cells. TEM results indicated that BSGLWE promoted the formation of apoptotic bodies and autophagosomes in HOS and U2 cells. Western blotting or immunofluorescence and rescue assay revealed that BSGLWE blocked autophagic flux by inducing initiation of autophagy and increasing autophagosome accumulation of osteosarcoma cells. BSGLWE not only repressed the angiogenesis in the mouse model, but also induced apoptosis and blocked autophagy in vivo. Conclusion: BSGLWE inhibits osteosarcoma progression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Water Extract of Sporoderm-Broken Spores of Ganoderma lucidum Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux

Zhang¹,

Zhong²,

Meng³

et al. 2019

OTT

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“…Recent studies strongly support the relevance of the interplay between autophagy and apoptosis. [22][23][24] Our result discovered that inhibition of autophagy via 3-MA increased the proportion of apoptotic 95D-Nrf2 cells, meaning that autophagy may potentially involve in Nrf2-associated activities in NSCLC. Then we detected changes of autophagy biomarkers in Nrf2 upregulated or downregulated cell lines, and these autophagy biomarkers dramatically changes in Nrf2 upregulated or downregulated cell lines.…”

Section: Discussionmentioning

confidence: 64%

Nrf2 promotes progression of non-small cell lung cancer through activating autophagy

Wang

Liu

et al. 2017

Cell Cycle

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The transcription factor, NFE2-related factor 2 (Nrf2) and autophagy have been implicated in the oxidative-stress response during tumor evolution. However, few studies focus on crosstalk between Nrf2 and autophagy in cancer progression of non-small cell lung cancer (NSCLC). Herein, we evaluated the effect of Nrf2 on autophagy in NSCLC and their role in development of NSCLC. Effect of Nrf2 on overal survival (OS) of NSCLC patients were evaluated. Cell biological behaviors in response to Nrf2 were evaluated by MTT, colony formation assay and flow cytometry. Effect of 3-MA (a classical inhibitor of autophagy) on 95D-Nrf2 cells was also analyzed using flow cytometry. After up/down-regulating Nrf2 in NSCLC cell lines, expression of autophagy-related proteins were evaluated with western blot analysis. The results revealed that Nrf2 was an independent prognositc factor negtively associated with OS of NSCLC patients. Elevated Nrf2 expression promotes NSCLC progression, enhancing the escape of tumor cells from apoptosis in vivo and in vitro. Double staining with Annexin V-APC and 7-AAD showed that the proportions of apoptotic cells in 95D-Nrf2 cells were gradually increased after the addition of 3-MA. Importently, Nrf2 induced autophagosome formation and enhanced autophagic activity, which subsequently inhibits NSCLC cell apoptosis. In conclusion, our present study demonstrates that Nrf2 promotes progression of non-small cell lung cancer through activating autophagy. It provides novel insights into Nrf2-mediated of cell proliferation in NSCLC and may facilitate therapeutic development against NSCLC.

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“…Apoptosis induction has been demonstrated in cancer cells cultured with peptides isolated from edible mushrooms (Patel & Goyal 2012 ; Dan et al. 2016 ). Due to its safety as a traditional food and its potential therapeutic effects, Lentinus squarrosulus Mont.…”

Section: Introductionmentioning

confidence: 99%

Peptides extracted from edible mushroom: Lentinus squarrosulus induces apoptosis in human lung cancer cells

Prateep

Sumkhemthong

Suksomtip

et al. 2017

Pharmaceutical Biology

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Context:Lentinus squarrosulus Mont. (Polyporaceae) is an interesting source of diverse bioactive compounds.Objective: This is the first study of the anticancer activity and underlying mechanism of peptides extracted from Lentinus squarrosuls.Materials and methods: Peptides were isolated from the aqueous extract of L. squarrosulus by employing solid ammonium sulphate precipitation. They were further purified by ion-exchange chromatography on diethylaminoethanol (DEAE)-cellulose and gel filtration chromatography on Sephadex G25. Anticancer activity was investigated in human lung cancer H460, H292 and H23 cells cultured with 0–40 μg/mL of peptide extracts for 24 h. Cell viability and mode of cell death were evaluated by MTT and nuclear staining assay, respectively. Western blotting was used to investigate the alteration of apoptosis-regulating proteins in lung cancer cells treated with peptide extracts (0–20 μg/mL) for 24 h.Results: The cytotoxicity of partially-purified peptide extracts from L. squarrosulus was indicated with IC50 of ∼26.84 ± 2.84, 2.80 ± 2.14 and 18.84 ± 0.30 μg/mL in lung cancer H460, H292 and H23 cells, respectively. The extracts at 20 μg/mL induced apoptosis through the reduction of anti-apoptotic Bcl-2 protein (∼0.5-fold reduction) and up-regulation of BAX (∼4.5-fold induction), a pro-apoptotic protein. Furthermore, L. squarrosulus peptide extracts (20 μg/mL) also decreased the cellular level of death receptor inhibitor c-FLIP (∼0.6-fold reduction).Conclusions and discussion: This study provides the novel anticancer activity and mechanism of L. squarrosulus peptide extracts, which encourage further investigation and development of the extracts for anticancer use.

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A Ribonuclease Isolated from Wild Ganoderma Lucidum Suppressed Autophagy and Triggered Apoptosis in Colorectal Cancer Cells

Cited by 28 publications

References 47 publications

<p>Water Extract of Sporoderm-Broken Spores of <em>Ganoderma lucidum</em> Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux</p>

<p>Water Extract of Sporoderm-Broken Spores of <em>Ganoderma lucidum</em> Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux</p>

Nrf2 promotes progression of non-small cell lung cancer through activating autophagy

Peptides extracted from edible mushroom: Lentinus squarrosulus induces apoptosis in human lung cancer cells

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