A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form beta-(1----2) glucan

Geremia, R.; Cavaignac, Sonia M.; Zorreguieta, Ángeles; Toro, Nicolás; Olivares, J.; Ugalde, Rodolfo A.

doi:10.1128/jb.169.2.880-884.1987

Cited by 105 publications

(91 citation statements)

References 28 publications

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“…Rhizobial mutants unable to form CG are severely impaired in their ability to nodulate determinate and indeterminate noduleforming legumes (Bhagwat et al 1992;Breedveld and Miller 1998;D'Antuono et al 2005;Fraysse et al 2003;Geremia et al 1987). In Sinohizobium meliloti, two genes, ndvA and ndvB, are necessary for CG production (Breedveld and Miller 1998).…”

Section: Sinorhizobium Fredii Hh103 Produces Cyclic β Glucans (Cg)mentioning

confidence: 99%

Sinorhizobium fredii HH103 cgs Mutants Are Unable to Nodulate Determinate- and Indeterminate Nodule–Forming Legumes and Overproduce an Altered EPS

Crespo‐Rivas¹,

Margaret²,

Hidalgo³

et al. 2009

MPMI

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Citation for published item:grespoE iv sD tFgF nd w rg retD sF nd rid lgoD ¡ eF nd fuend¡ % Egl ver¡ % D eFwF nd ylleroD pFtF nd v¡ opezEf en D pFtF nd wurdo hD FhF F nd odr¡ %guezEg rv j lD wFeF nd ori Eh¡ % zD wFiF nd eguer D wF nd vloretD tF nd umptonD hF F nd woselyD tFeF nd hom sEy tesD tFiF nd v n frusselD eFeFxF nd qilE err noD eF nd in rdellD tFwF nd uizE inzD tFiF @PHHWA 9 inorhizo ium fredii rrIHQ gs mut nts re un le to nodul te determin teE nd indetermin te nodule!forming legumes nd overprodu e n ltered i F9D wole ul r pl ntEmi ro e inter tionsFD PP @SAF ppF SUSESVVF Further information on publisher's website: httpXGGdxFdoiForgGIHFIHWRGw wsEPPESEHSUS Publisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details.

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Section: Sinorhizobium Fredii Hh103 Produces Cyclic β Glucans (Cg)mentioning

confidence: 99%

Sinorhizobium fredii HH103 cgs Mutants Are Unable to Nodulate Determinate- and Indeterminate Nodule–Forming Legumes and Overproduce an Altered EPS

Crespo‐Rivas¹,

Margaret²,

Hidalgo³

et al. 2009

MPMI

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“…While these glycans are comprised exclusively of glucose, they can be structurally diverse, and four distinct families were defined based on variations in chain length, glycosyl linkage, branching pattern, and presence of modifications (1). In addition, periplasmic glucans were reported to be important for biofilm development (3), antibiotic resistance (4), host-pathogen and plant-microbe interactions (5)(6)(7)(8)(9), as well as intracellular multiplication (10). Free oligosaccharides (fOS) have also been described in mammalian cells, yeast, plants, and fish (11).…”

mentioning

confidence: 99%

Study of free oligosaccharides derived from the bacterial N -glycosylation pathway

Nothaft

Liu

McNally

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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The food-borne pathogen Campylobacter jejuni is one of the leading causes of bacterial gastroenteritis worldwide and the most frequent antecedent in neuropathies such as the Guillain-Barré and Miller Fisher syndromes. C. jejuni was demonstrated to possess an N-linked protein glycosylation pathway that adds a conserved heptasaccharide to >40 periplasmic and membrane proteins. Recently, we showed that C. jejuni also produces free heptasaccharides derived from the N-glycan pathway reminiscent of the free oligosaccharides (fOS) produced by eukaryotes. Herein, we demonstrate that C. jejuni fOS are produced in response to changes in the osmolarity of the environment and bacterial growth phase. We provide evidence showing the conserved WWDYG motif of the oligosaccharyltransferase, PglB, is necessary for fOS release into the periplasm. This report demonstrates that fOS from an N-glycosylation pathway in bacteria are potentially equivalent to osmoregulated periplasmic glucans in other Gram-negative organisms.Campylobacter jejuni ͉ N-linked protein glycosylation ͉ periplasmic glucans ͉ osmolarity

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“…We have no clear answer to this apparent paradox, but two testable models are presented here. One is that, like R. meliloti (Glazebrook & Walker, 1989), A . radiobacter has the potential to make two types of EPS molecule but that normally the synthesis of one of these is suppressed.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, both R. rneliloti and Agrobacterium tumefaciens make an extracellular polysaccharide cyclic 1,2-/?-~-glucan, that is required for normal nodule formation and the induction of crown galls respectively. In R. meliloti, two genes, ndvA and ndvB are required for the manufacture and export of this glucan (Geremia et al, 1987) and in A . turnefaciens chvA and chvB are responsible for its synthesis and export, respectively.…”

Section: Introductionmentioning

confidence: 99%

Identification of the exo loci required for exopolysaccharide synthesis in Agrobacterium radiobacter NCIB 11883

Aird¹,

Brightwell²,

Jones³

et al. 1991

Journal of General Microbiology

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We initiated a genetic analysis of Agrobacterium radiobacter NCIB 11883 with particular reference to the (exo) genes required for exopolysaccharide synthesis. Following mutagenesis with nitrosoguanidine, several ex0 mutant strains were isolated and several of the mutations were corrected by DNA cloned in a newly constructed cosmid library. Analysis of various complementing cosmids by genetic and physical criteria indicated that ex0 loci were quite widely dispersed in the bacterial genome. Certain ex0 mutations were corrected by different cosmids that shared no homologous DNA; possible explanations for this are presented. UsingphoA fusions, it was shown that some ex0 genes were, or were closely linked to, genes that specified polypeptides associated with the bacterial cell surface. By introducing the cloned ex0 genes of Rhizobium melifoti it was found that only one out of thirty ex0 mutants of A. rudiobacter was corrected by a defined ex0 locus of the former species; further analysis indicated that this particular ex0 gene corresponded to exoB of R. mefiloti. Finally, it was found that several A. radiobacter ex0 mutants were non-mucoid on media with dicarboxylic acids as sole carbon source but appeared to be wild-type when sugars were the source of carbon.

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A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form beta-(1----2) glucan

Cited by 105 publications

References 28 publications

Sinorhizobium fredii HH103 cgs Mutants Are Unable to Nodulate Determinate- and Indeterminate Nodule–Forming Legumes and Overproduce an Altered EPS

Sinorhizobium fredii HH103 cgs Mutants Are Unable to Nodulate Determinate- and Indeterminate Nodule–Forming Legumes and Overproduce an Altered EPS

Study of free oligosaccharides derived from the bacterial N -glycosylation pathway

Identification of the exo loci required for exopolysaccharide synthesis in Agrobacterium radiobacter NCIB 11883

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