A revisit of high collision energy effects on collision‐induced dissociation spectra using matrix‐assisted laser desorption/ionization tandem time‐of‐flight mass spectrometry (MALDI‐LIFT‐TOF/TOF): application to the sequencing of RNA/DNA chimeras

Mauger, Florence; Tabet, Jean‐Claude; Gut, Marta

doi:10.1002/rcm.6913

Cited by 4 publications

(2 citation statements)

References 36 publications

(108 reference statements)

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“…Application of the MALDI TOF/TOF allowed for observation of further fragmentation of the molecular ions. This technique successfully identifies macromolecular compounds such as proteins, peptides, DNA, and polymers [ 45 , 46 , 47 ]. Exemplary mass spectra of compound 3 are shown in Figure S33 , with masses noted for the most abundant ions.…”

Section: Resultsmentioning

confidence: 99%

Novel Short PEG Chain-Substituted Porphyrins: Synthesis, Photochemistry, and In Vitro Photodynamic Activity against Cancer Cells

Łażewski

Kucińska

Potapskiy

et al. 2022

IJMS

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This work presents the synthesis and characterization of metal-free, zinc (II), and cobalt (II) porphyrins substituted with short PEG chains. The synthesized compounds were characterized by UV-Vis, 1H and 13C NMR spectroscopy, and MALDI-TOF mass spectrometry. The origin of the absorption bands for tested compounds in the UV-Vis range was determined using a computational model based on the electron density functional theory (DFT) and its time-dependent variant (TD-DFT). The photosensitizing activity was evaluated by measuring the ability to generate singlet oxygen (ΦΔ), which reached values up to 0.54. The photodynamic activity was tested using bladder (5637), prostate (LNCaP), and melanoma (A375) cancer cell lines. In vitro experiments clearly showed the structure–activity relationship regarding types of substituents, their positions in the phenyl ring, and the variety of central metal ions on the porphyrin core. Notably, the metal-free derivative 3 and its zinc derivative 6 exerted strong cytotoxic activity toward 5637 cells, with IC50 values of 8 and 15 nM, respectively. None of the tested compounds induced a cytotoxic effect without irradiation. In conclusion, these results highlight the potential value of the tested compounds for PDT application.

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Section: Resultsmentioning

confidence: 99%

Novel Short PEG Chain-Substituted Porphyrins: Synthesis, Photochemistry, and In Vitro Photodynamic Activity against Cancer Cells

Łażewski

Kucińska

Potapskiy

et al. 2022

IJMS

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“…The path of decomposition generated in the ionization process quasimolecular ions [M+H] + of new octa- and tetrasubstituted Pcs was studied. For this task the MALDI-LIFT-TOF technique was chosen, which was used successfully in the identification of macromolecular compounds such as proteins, peptides DNA and polymers [ 40 , 41 , 42 ]. Scheme 2 A presents the observed fragmentation of parent ions [M+H] + .…”

Section: Resultsmentioning

confidence: 99%

New Metallophthalocyanines Bearing 2-Methylimidazole Moieties—Potential Photosensitizers against Staphylococcus aureus

Wierzchowski

Ziental

Łażewski

et al. 2022

IJMS

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Newly developed tetra- and octasubstituted methimazole-phthalocyanine conjugates as potential photosensitizers have been obtained. Synthesized intermediates and final products were characterized by the MALD-TOF technique and various NMR techniques, including 2D methods. Single-crystal X-ray diffraction was used to determine the crystal structures of dinitriles. The studied phthalocyanines revealed two typical absorption bands—the Soret band and the Q band. The most intense fluorescence was observed for octasubstituted magnesium(II) phthalocyanine in DMF (ΦFL = 0.022). The best singlet oxygen generators were octasubstituted magnesium(II) and zinc(II) phthalocyanines (Φ∆ 0.56 and 0.81, respectively). The studied compounds presented quantum yields of photodegradation at the level between 10−5 and 10−6. Due to their low solubility in a water environment, the liposomal formulations were prepared. Within the studied group, octasubstituted zinc(II) phthalocyanine at the concentration of 100 µM activated with red light showed the highest antibacterial activity against S. aureus equal to a 5.68 log reduction of bacterial growth.

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Engineering digital polymer based on thiol–maleimide Michael coupling toward effective writing and reading

Liu

Shi

et al. 2020

Polym. Chem.

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A revisit of high collision energy effects on collision‐induced dissociation spectra using matrix‐assisted laser desorption/ionization tandem time‐of‐flight mass spectrometry (MALDI‐LIFT‐TOF/TOF): application to the sequencing of RNA/DNA chimeras

Cited by 4 publications

References 36 publications

Novel Short PEG Chain-Substituted Porphyrins: Synthesis, Photochemistry, and In Vitro Photodynamic Activity against Cancer Cells

Novel Short PEG Chain-Substituted Porphyrins: Synthesis, Photochemistry, and In Vitro Photodynamic Activity against Cancer Cells

New Metallophthalocyanines Bearing 2-Methylimidazole Moieties—Potential Photosensitizers against Staphylococcus aureus

Engineering digital polymer based on thiol–maleimide Michael coupling toward effective writing and reading

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