A REVISION OF THE GEOGRAPHICAL DISTRIBUTIONS OF THE SOUTHEAST ASIAN SHREWS (Crocidura dracula &amp; C. fuliginosa) BASED ON NEW COLLECTION IN VIETNAM

Hai, Bui Tuan; Motokawa, Masaharu; Hoa, Ninh Thi; Canh, Le Xuan

doi:10.15625/vap.2020.0001

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“…These genes were amplified using the 2× Taq plus PCR Master Mix kit (Biosharp, Anhui, China). The primers used were SoriR (5'-TGACATGAAAAATCATCGTTG-3') and SoriF (5'-CCATCTCTGGTTTACAAGAC-3') for cytb (Bui et al 2020a); Rag1F1851 (5'-ACATGGAAGAAGACATCTTGGAAGG-3') and Rag1R2486 (5'-AATGTCACAGTGAAGGGCATCTATGGAAGG-3') for RAG1 (Sato et al 2004); RAG2-F220 (5'-GATTCCTGCTAYCTYCCTCCTCT-3') and RAG2-R995 (5'-CCCATGTTGCTTCCAAACCATA-3') for RAG2 (Teeling et al 2000). Fragments of these sequences were amplified under the following PCR conditions: 94°C for 5 min; 40 cycles at 94°C for 30 s, 52°C for 30 s, and 72 °C for 2 min; followed by a final extension step at 72°C for 7 min for cytb; and 95°C for 5 min; 35 cycles at 95°C for 30 s, 56.8-61.6°C for 45 s, and 72°C for 1 min; 72°C for 10 min for RAG1 and RAG2.…”

Section: Methodsmentioning

confidence: 99%

A new shrew mole species of the genus Uropsilus (Eulipotyphla: Talpidae) from northwestern Vietnam

BUI,

OKABE,

et al. 2023

Zootaxa

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A new medium-sized shrew mole species of the genus Uropsilus from Mount Fansipan, Hoang Lien National Park, Lao Cai Province, northwestern Vietnam is described based on morphological and molecular differences. Uropsilus fansipanensis sp. nov. is distinguished from the other Uropsilus species by the combination of the following features: the dorsum is lightly reddish-brown and venter is dark gray; the dark gray tail is long and slender, with a scattered white base and short bristle hairs; orbital process is oriented upwards posteriorly; lacrimal foramen is well developed and much larger than infraorbital foramen; the lower first premolar is approximately the same size as the lower third premolar. Genetic distances in terms of mitochondrial cytochrome b from other Uropsilus species presented pairwise divergences from 8.63 to 20.70%. To date, the new species is known to exist only in the type locality of Mt. Fansipan, a wet and cold temperate climate area with an upper montane forest at an elevation of approximately 2900 m, forming the southernmost distribution of the genus Uropsilus.

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Section: Methodsmentioning

confidence: 99%