A revised method for the observation of conidiogenous structures in fungi

Nugent, L. K.; Sangvichen, Ek; Sihanonth, Prakitsin; Ruchikachorn, Nutthaporn; Whalley, A.J.S.

doi:10.1016/j.mycol.2006.06.003

Cited by 22 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the measurements of bilaterally flattened ascospores, the size was reported as “length” × “width in front view” × “width in lateral view”. To study the asexual spore development, slides were prepared using the inclined coverslip method ( Kawato & Shinobu 1959 , revised in Nugent et al. 2006 ).…”

Section: Methodsmentioning

confidence: 99%

Redefining Humicola sensu stricto and related genera in the Chaetomiaceae

Wang

Yang

Meijer

et al. 2019

Studies in Mycology

View full text Add to dashboard Cite

The traditional concept of the genus Humicola includes species that produce pigmented, thick-walled and single-celled spores laterally or terminally on hyphae or minimally differentiated conidiophores. More than 50 species have been described in the genus. Species commonly occur in soil, indoor environments, and compost habitats. The taxonomy of Humicola and morphologically similar genera is poorly understood in modern terms. Based on a four-locus phylogeny, the morphological concept of Humicola proved to be polyphyletic. The type of Humicola, H. fuscoatra, belongs to the Chaetomiaceae. In the Chaetomiaceae, species producing humicola-like thick-walled spores are distributed among four lineages: Humicola sensu stricto, Mycothermus, Staphylotrichum, and Trichocladium. In our revised concept of Humicola, asexual and sexually reproducing species both occur. The re-defined Humicola contains 24 species (seven new and thirteen new combinations), which are described and illustrated in this study. The species in this genus produce conidia that are lateral, intercalary or terminal on/in hyphae, and conidiophores are not formed or are minimally developed (micronematous). The ascospores of sexual Humicola species are limoniform to quadrangular in face view and bilaterally flattened with one apical germ pore. Seven species are accepted in Staphylotrichum (four new species, one new combination). Thick-walled conidia of Staphylotrichum species usually arise either from hyphae (micronematous) or from apically branched, seta-like conidiophores (macronematous). The sexual morph represented by Staphylotrichum longicolleum (= Chaetomium longicolleum) produces ascomata with long necks composed of a fused basal part of the terminal hairs, and ascospores that are broad limoniform to nearly globose, bilaterally flattened, with an apical germ pore. The Trichocladium lineage has a high morphological diversity in both asexual and sexual structures. Phylogenetic analysis revealed four subclades in this lineage. However, these subclades are genetically closely related, and no distinctive phenotypic characters are linked to any of them. Fourteen species are accepted in Trichocladium, including one new species, twelve new combinations. The type species of Gilmaniella, G. humicola, belongs to the polyphyletic family Lasiosphaeriaceae (Sordariales), but G. macrospora phylogenetically belongs to Trichocladium. The thermophilic genus Mycothermus and the type species My. thermophilum are validated, and one new Mycothermus species is described. Phylogenetic analyses show that Remersonia, another thermophilic genus, is sister to Mycothermus and two species are known, including one new species. Thermomyces verrucosus produces humicola-like conidia and is transferred to Botryotrichum based on phylogenetic affinities. This study is a first attempt to establish an inclusive modern classification of Humicola and humicola-like genera of the Chaetomiaceae. More research is needed to determine the phylogenetic relationships of “humicola”-like species outsid...

show abstract

Section: Methodsmentioning

confidence: 99%

Redefining Humicola sensu stricto and related genera in the Chaetomiaceae

Wang

Yang

Meijer

et al. 2019

Studies in Mycology

View full text Add to dashboard Cite

show abstract

“…Morphological observations of reproductive structures were determined using a Nikon Eclipse 80i compound microscope with differential interference contrast (DIC) illumination. Slides were prepared using the inclined coverslip method ( Kawato & Shinobu 1959 , revised in Nugent et al. 2006 ) and also transparent adhesive tape (Titan Ultra Clear Tape, Conglom Inc., Toronto, Canada) ( Bensch et al.…”

Section: Methodsmentioning

confidence: 99%

All that glitters is not Ramularia

Videira

Groenewald

Braun

et al. 2016

Studies in Mycology

113

View full text Add to dashboard Cite

Ramularia is a species-rich genus that harbours plant pathogens responsible for yield losses to many important crops, including barley, sugar beet and strawberry. Species of Ramularia are hyphomycetes with hyaline conidiophores and conidia with distinct, thickened, darkened, refractive conidiogenous loci and conidial hila, and Mycosphaerella sexual morphs. Because of its simple morphology and general lack of DNA data in public databases, several allied genera are frequently confused with Ramularia. In order to improve the delimitation of Ramularia from allied genera and the circumscription of species within the genus Ramularia, a polyphasic approach based on multilocus DNA sequences, morphological and cultural data were used in this study. A total of 420 isolates belonging to Ramularia and allied genera were targeted for the amplification and sequencing of six partial genes. Although Ramularia and Ramulariopsis proved to be monophyletic, Cercosporella and Pseudocercosporella were polyphyletic. Phacellium isolates clustered within the Ramularia clade and the genus is thus tentatively reduced to synonymy under Ramularia. Cercosporella and Pseudocercosporella isolates that were not congeneric with the ex-type strains of the type species of those genera were assigned to existing genera or to the newly introduced genera Teratoramularia and Xenoramularia, respectively. Teratoramularia is a genus with ramularia-like morphology belonging to the Teratosphaeriaceae, and Xenoramularia was introduced to accommodate hyphomycetous species closely related to Zymoseptoria. The genera Apseudocercosporella, Epicoleosporium, Filiella, Fusidiella, Neopseudocercosporella, and Mycosphaerelloides were also newly introduced to accommodate species non-congeneric with their purported types. A total of nine new combinations and 24 new species were introduced in this study.

show abstract

“…4 , 5 , 6 , 7 , 8 , 9 ). Slides were prepared using the inclined coverslip method ( Kawato & Shinobu 1959 , Nugenta et al 2006 ) and also with transparent adhesive tape (Titan Ultra Clear Tape, Conglom Inc., Toronto, Canada) ( Bensch et al 2012 ). Lactic acid (clear) was used as mounting medium for the measurements and Lactophenol cotton blue was used in some preparations to improve the contrast of the naturally hyaline structures.…”

Section: Maldi-tof Msmentioning

confidence: 99%

Elucidating the <I>Ramularia eucalypti</I> species complex

Videira¹,

Groenewald²,

Kolecka³

et al. 2015

Pers - Int Mycol J

View full text Add to dashboard Cite

The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as R. eucalypti based on morphology and on nrDNA sequence data of the ITS region have recently been isolated from other plant hosts, from environmental samples and also from human clinical specimens. Identification of closely related species based on morphology is often difficult and the ITS region has previously been shown to be unreliable for species level identification in several genera. In this study we aimed to resolve this species-complex by applying a polyphasic approach involving morphology, multi-gene phylogeny and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Six partial genes (ITS, ACT, TEF1-α, HIS3, GAPDH and RPB2) were amplified and sequenced for a total of 44 isolates representing R. eucalypti s.lat. and closely related species. A multi-gene Bayesian phylogenetic analysis and parsimony analysis were performed, and both the resulting trees showed significant support for separation of seven species in R. eucalypti s.lat., including two previously described (R. eucalypti and R. miae), four novel species here described (R. haroldporteri, R. glennii, R. mali and R. plurivora) and one undescribed Ramularia species (sterile). Additionally, Mycosphaerella nyssicola is newly combined in Ramularia as R. nyssicola. Main mass spectra (MSPs) of several R. eucalypti s.lat. strains were generated using MALDI-TOF MS and were compared through a Principal Component Analysis (PCA) dendogram. The PCA dendrogram supported three clades containing R. plurivora, R. glenni/R. mali and R. eucalypti/R. miae. Although the dendrogram separation of species differed from the phylogenetic analysis, the clinically relevant strains were successfully identified by MALDI-TOF MS.

show abstract

A revised method for the observation of conidiogenous structures in fungi

Cited by 22 publications

References 4 publications

Redefining Humicola sensu stricto and related genera in the Chaetomiaceae

Redefining Humicola sensu stricto and related genera in the Chaetomiaceae

All that glitters is not Ramularia

Elucidating the <I>Ramularia eucalypti</I> species complex

Contact Info

Product

Resources

About