A revised experimental system of root-soil interaction for laboratory work.

Kono, Yasuhiro; Yamauchi, Akira; Nonoyama, Toshihiro; Tatsumi, Jiro; Kawamura, Norio

doi:10.2525/ecb1963.25.141

Cited by 72 publications

(63 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2 seedlings of maize (Anova, Tina) and triticale (CHD147, CHD-247) were grown in rootboxes, what enabled the non-destructive isolation of all components of the root system (Kono et al 1987a). The set of a ''root-box and pin board method'' consists of: a plexiglass root box (width, 0.25 m; depth, 0.40 m; thickness, 0.02 m), a pin board for sampling the root system, and a polyethylene sheet (envelope) for handling and preserving the root system.…”

Section: Growth Conditionsmentioning

confidence: 99%

Interspecific differences in root architecture among maize and triticale genotypes grown under drought, waterlogging and soil compaction

Grzesiak

Ostrowska

Hura³

et al. 2014

Acta Physiol Plant

View full text Add to dashboard Cite

Environmental stresses (soil compaction, drought, waterlogging) cause changes in plants' root system structure, also affecting the growth of above-ground parts. The aim of this study was to estimate phenotypic variation among maize and triticale genotypes in root penetration ability through petrolatum-wax-layer (RPA). Also, the effect of shortage or excess of soil water on dry matter of shoots and roots and morphological changes in root system structure in sensitive and resistant maize and triticale genotypes grown in low or high soil compaction level was evaluated. To estimate RPA index, the petrolatum-wax-layer method (PWL) was used. The strength of three petrolatum-wax concentrations 60, 50 and 40 % was 0.52, 1.07 and 1.58 MPa, respectively. High coefficients of variation (CV) were observed in 0.52 and 1.07 MPa and for maize were 19.2 and 21.7 %, and for triticale, 12.5 and 18.3 %, respectively. The data indicate that the use of PWL technique is an effective screening method, and makes it possible to divide the genotypes into resistant and sensitive groups. The second part of this study investigated a multistress effect of soil compaction combined with drought or waterlogging on root and shoot growth and morphological changes in root system structure of maize and triticale genotypes differing in susceptibility to environmental stresses. Seedlings were grown for 4 weeks in root-boxes under conditions of low (LSC 1.1 g cm -3 ) or severe (SSC 1.6 g cm -3 ) soil compaction. Drought or waterlogging stresses were applied for 2 weeks from 14th to 28th day. In comparison to LSC treatment, in SSC treatment the decrease in dry matter of shoots and roots was greater for sensitive genotypes of maize and triticale (Ancora, CHD-147). Soil drought or waterlogging caused greater decrease of dry matter of shoots and roots in seedlings grown in SSC in comparison to LSC. The root penetration index (RPI) was estimated as a ratio of root dry matter in 15-40 cm root-box layer to total root dry matter. On the basis of RPI it was possible to group the genotypes according to their ability to distribute roots in soil profile. In comparison to LSC, SSC exerted a strong influence on the length of seminal and seminal adventitious roots, as well as the number and length of L-and S-type lateral roots developed on seminal and nodal roots. In both species the restriction effect of soil compaction on number and length of roots was more severe in sensitive (Ankora, CHD-147) than in resistant (Tina, CHD-247) genotypes. The restriction in roots propagation was greater in triticale than in maize. Exposure to drought or waterlogging in the case of genotypes grown in LSC and SSC treatments caused a decrease in number and length of particular components of root system structure. In both species the decrease of root number and length in plants grown under waterlogging was greater than under drought. The observed changes in root system were greater in sensitive (Ankora, CHD147) than in resistant (Tina, CHD-247) genotypes. Statistically signif...

show abstract

Section: Growth Conditionsmentioning

confidence: 99%

Interspecific differences in root architecture among maize and triticale genotypes grown under drought, waterlogging and soil compaction

Grzesiak

Ostrowska

Hura³

et al. 2014

Acta Physiol Plant

View full text Add to dashboard Cite

show abstract

“…Soil substrate pH was 7.1 and the percent of organic material was 0.7 %. For the determination of root length and number of root system components, seedlings were grown in root-boxes, which enabled non-destructive isolation of all compartments of the root system (Kono et al 1987). A set for the ''root-box and pin board method'' consists of: a Plexiglas box (width-0.25 m, depth-0.40 m, thickness-0.02 m), a pin board for sampling the root system, and a polyethylene sheet (envelope) for handling and preserving the root system.…”

Section: Experimental Conditionsmentioning

confidence: 99%

The relations between drought susceptibility index based on grain yield (DSIGY) and key physiological seedling traits in maize and triticale genotypes

et al. 2012

View full text Add to dashboard Cite

The physiological reasons for the differences in sensitivity of C 3 and C 4 plant species to environmental stresses have not been thoroughly explained. In this study the effects of drought stress on the growth and selected physiological traits were examined in the seedlings of 13 single cross maize (C 4 plant) hybrids and 11 spring triticale (C 3 plant) breeding lines and varieties differing in drought sensitivity. For plants in the seedling stage the results demonstrated a genetic variation in dry matter accumulation of shoots and roots (DW S , DW R ), number (N) and length (L) of particular components (seminal, seminal adventitious, nodal) of the root system, membrane injury by soil drought (LI D ), osmotic and high temperature stress (LI OS , LI HT ), water potential (w), water loss (WL), grain germination in osmotic stress (FG, PI), and seedling survival (SS). Seedlings grown under moderate soil drought showed a decrease in dry matter of the top parts and roots and a decrease in the length of seminal, seminal adventitious and nodal roots in comparison to seedlings grown in control conditions. The observed harmful effects of drought stress were more distinct in drought sensitive genotypes. Used in this paper drought susceptibility indexes (DSI GY ) were calculated in other experiment by determining the changes in grain yield (GY) under two soil moisture levels (irrigated and drought). The variation of DSI GY for maize ranges from 0.381 to 0.650 and for triticale from 0.354 to 0.578. The correlations between DSI GY and laboratory tests (LI, FG, SS) confirmed that they are good indicators of drought tolerance in plants. The highest values of genetic variation were observed in LI, DW S , SS and WL and the lowest in the measurements of w FG, PI, L S , L SA and L N . The correlation coefficients between LI OS and LI HT tests were, in most of the considered cases, statistically significant, which indicates that in maize and triticale the mechanisms of membrane injury caused by simulated drought or high temperature are physiologically similar. It can be concluded that an approach to the breeding of maize and triticale for drought tolerance using these tests can be implemented on the basis of separate selection for each trait or for all of them simultaneously. In that case, it would be necessary to determine the importance of the trait in relation to growth phase, drought timing and level, as well as its associations with morphological traits contributing to drought tolerance. The obtained values of the correlation coefficient between laboratory tests suggest that the same physiological traits may be applied as selection criteria in drought tolerance of maize and triticale genotypes.

show abstract

“…Leaf blade area was measured using a leaf area meter (LI 3100, Li-Cor, Lincoln, Nebraska). Each root system was sampled by the pin-board method modifi ed by Kono et al (1987).…”

Section: Sampling Proceduresmentioning

confidence: 99%