A review: Radiation sterilization of pharmaceuticals

Jacobs, G.P.

doi:10.1016/0146-5724(85)90177-3

Cited by 46 publications

(28 citation statements)

References 42 publications

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“…However, when pullulanase was immobilized onto different polymeric supports, the relative activities did not suffer any loss up to a dose of 1.5 Mrad. 6 Increasing the irradiation dose led to a small decrease in relative activity of the immobilized enzyme. At a dose of 4 Mrad the agarose±trichloro-triazine preparation lost 8% of its initial activity, while the enzymes immobilized onto the other carriers lost 15±20% of their initial activity.…”

Section: Effect Of G-radiationmentioning

confidence: 98%

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Pullulanase immobilization on natural and synthetic polymers

Dessouki

Issa²,

Atia³

2001

J of Chemical Tech & Biotech

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This work describes the immobilization of pullulanase onto two different polymers; agarose activated with epichlorohydrin and trichlorotriazine and casein activated with epichlorohydrin, in addition to a synthetic copolymer, butylacrylate±acrylic acid (BuA/AAc). Immobilization by covalent binding yields stable enzyme activity. The operational stability of the free and immobilized enzymes showed that the enzyme immobilized by a crosslinking technique using glutaric dialdehyde (GA) showed poor durability and the relative activity decreased sharply due to leakage after repeated washing, while the enzymes immobilized by covalent bonds resulted in a slight decrease in most cases in the relative activity (around 20%) after being used 10 times. Storage for 4±6 months showed that the free enzyme lost most of its activity, while the immobilized enzyme showed the opposite behavior. Subjecting the immobilized enzymes to doses of g-radiation (0.5±10 Mrad) resulted in complete loss in the activity of the free enzyme at a dose of 5 Mrad, while the immobilized enzymes showed relatively high resistance to g-radiation up to a dose of 5 Mrad. Nuclear Magnetic Resonance ( 1 H NMR) and FTIR measurements were carried out to con®rm the structure of the polymer as well as the immobilization process of the enzyme onto the polymeric carrier. The unique biochemical characteristics, mode of action and utility of the environmentally compatible pullulanase in starch conversion are well known. Using pullulanase with b-amylase in starch sacchari®cation processes can increase maltose yield by 20±25%.

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Section: Effect Of G-radiationmentioning

confidence: 98%

“…The probability of sterilization by gamma-radiation was studied on both free and immobilized enzyme, since sterilization by heat, which is a widely used procedure, is completely inapplicable in this case. 6 …”

Section: Introductionmentioning

confidence: 97%

Pullulanase immobilization on natural and synthetic polymers

Dessouki

Issa²,

Atia³

2001

J of Chemical Tech & Biotech

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“…For the accumulation of trapped radicals, the yields start to be reduced from 15 to 25 kGy (5,6). The effects on yields of peaks 20 and 26 are similar as expected; the radicals are the intermediate in the production of final products.…”

Section: Dose Effectmentioning

confidence: 99%

“…Gas-chromatographic techniques may be used to identify "unique" volatile compounds characteristic of the radiosterilization of solid forms (3). HPLC (high performance liquid chromatography) is also useful for studying final radiolytic products (4), some of which are "unique" and characteristic of radiolysis (3,4,5).…”

Section: Introductionmentioning

confidence: 99%

Detection of the radiolysis of solid ampicillin by UV - spectroscopy

Gibella

Tilquin

1999

Analusis

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Abstract. We propose to detect the use of γ-rays in order to radiosterilize ampicillin by UV absorption spectroscopy. The UV absorption results are verified by comparison with HPLC-diode array analysis. The sensitivity is excellent (10 -6 M). The wavelengths in the range from 287 to 335 nm are characteristics of radiolysis related substances. It is possible to use this range of wavelengths to detect the radiolysis of ampicillin with an usual UV spectrophotometer.

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“…Ionizing radiation treatment is a useful method to sterilize and measure radiation doses of drugs and medical devices, cleaning the disinfection of foodstuffs and cosmetics [1][2][3][4][5][6][7][8]. EPR spectroscopy is used for the detection of free radicals formed in biological and chemical systems, or in irradiated drugs [9].…”

Section: Introductionmentioning

confidence: 99%

Cilt: 3, No: 2, 2016

Osmanoğlu

Hamadameen

Yeşil

et al. 2016

El-Cezeri Fen Ve Mühendislik Dergisi

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In this study, Electron paramagnetic resonance (EPR) spectroscopy was used to determine the gamma radiation damages in Lamotrigine and Flurbiprofen.The free radicals produced in the samples were interpreted in terms of some type of alkyl and amine radical fragments. The g values and hyperfine structure constants of the radicals were determined and the spectra were computer simulated. The free radicals were appeared to be stable at room temperature for more than two months.

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A review: Radiation sterilization of pharmaceuticals

Cited by 46 publications

References 42 publications

Pullulanase immobilization on natural and synthetic polymers

Pullulanase immobilization on natural and synthetic polymers

Detection of the radiolysis of solid ampicillin by UV - spectroscopy

Cilt: 3, No: 2, 2016

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