A review on the use of fluorescamine as versatile and convenient analytical probe

Derayea, Sayed M.; Samir, Ebtihal

doi:10.1016/j.microc.2020.104835

Cited by 26 publications

(7 citation statements)

References 102 publications

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“…As shown in Figure 2 B, it was necessary to evaluate the kinetic period before obtaining the calibration curve. After that, the measure of SN binding to F127 was determined, showing an ending group amine conversion of 32% for F127, a value very similar to those reported in the literature for synthesis involving polyamines [ 49 , 55 , 56 ]. Finally, the quantification of FA conjugated to the copolymer F127 was measured using a calibration curve performed with FA in DMSO (11% of yield).…”

Section: Resultssupporting

confidence: 66%

“…The percentage of SN binding in the F127 was determined using the fluorescamine assay [ 49 , 55 ]. Fluorescamine is a nonfluorescent compound that reacts with primary amines forming a fluorescent species.…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescamine is a nonfluorescent compound that reacts with primary amines forming a fluorescent species. This species allows the detection of the presence and the quantitative determination of primary amines [ 55 , 56 ]. Therefore, this technique is well known for being selective and sensitive to the amine content in the sample, thus, the synthetic product.…”

Section: Resultsmentioning

confidence: 99%

“…The copolymeric micelles (1.0% w/v ) were obtained as previously described with one step of modification, the addition of 7 µmol/L of DPH. The absorption spectra of DPH were collected in the 300–500 nm range after 20 min of thermal stabilization at 15.0–50.0 °C and allowed the CMT determination (λ max = 356 nm) [ 53 , 54 , 55 , 56 , 57 ]. The F127 without modification was used as standard.…”

Section: Methodsmentioning

confidence: 99%

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Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma

et al. 2022

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Biotin, spermine, and folic acid were covalently linked to the F127 copolymer to obtain a new drug delivery system designed for HY-loaded PDT treatment against B16F10 cells. Chemical structures and binders quantification were performed by spectroscopy and spectrophotometric techniques (1NMR, HABA/Avidin reagent, fluorescamine assay). Critical micelle concentration, critical micelle temperature, size, polydispersity, and zeta potential indicate the hydrophobicity of the binders can influence the physicochemical parameters. Spermine-modified micelles showed fewer changes in their physical and chemical parameters than the F127 micelles without modification. Furthermore, zeta potential measurements suggest an increase in the physical stability of these carrier systems. The phototherapeutic potential was demonstrated using hypericin-loaded formulation against B16F10 cells, which shows that the combination of the binders on F127 copolymer micelles enhances the photosensitizer uptake and potentializes the photodynamic activity.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma

et al. 2022

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“…Fluorescamine refers to a biochemical reagent that is effective for primary amines. The complex formed by the reaction with primary amines is a prominent fluorescent substance [ 42 , 43 ]. The reaction is performed at an ambient temperature, and the medium can be an aqueous solution or organic solution.…”

Section: Introductionmentioning

confidence: 99%

Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu2+ in Cells

Ren,

Li,

Han

et al. 2024

Molecules

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Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu2+. With the combination of Cu2+, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu2+ with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu2+ perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu2+ complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu2+ to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu2+ in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu2+ in cells, and it has promising applications in the mechanism research and diagnosis of Cu2+-associated diseases.

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Target‐Fluorophore Binding

2023

Fluorescent Dye Labels and Stains

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A review on the use of fluorescamine as versatile and convenient analytical probe

Cited by 26 publications

References 102 publications

Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma

Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma

Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu2+ in Cells

Target‐Fluorophore Binding

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