A review on the role of miR-210 in human disorders

Khalilian, Sheyda; Bijanvand, Atefeh; Abedinlou, Hamid; Ghafouri‐Fard, Soudeh

doi:10.1016/j.prp.2022.154244

Cited by 12 publications

(12 citation statements)

References 38 publications

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“…It is found in underexpression in bladder cancer, angiosarcoma, and glioblastoma. Although, it is upregulated in breast cancer ( Khalilian et al, 2023 ), There is also an increase in expression levels of miR-210 and BRCA1 mRNA in TNBC patients after treatment and with a worse prognosis ( Boukerroucha et al, 2015 ). miR-210 participates in the proliferation of breast cancer cells, particularly triple-negative cells, and is related to lymph node metastases through targets such as CCND1 and RUNX3 ( Khalilian et al, 2023 ; Santana et al, 2023 ).…”

Section: Resultsmentioning

confidence: 99%

“…Although, it is upregulated in breast cancer ( Khalilian et al, 2023 ), There is also an increase in expression levels of miR-210 and BRCA1 mRNA in TNBC patients after treatment and with a worse prognosis ( Boukerroucha et al, 2015 ). miR-210 participates in the proliferation of breast cancer cells, particularly triple-negative cells, and is related to lymph node metastases through targets such as CCND1 and RUNX3 ( Khalilian et al, 2023 ; Santana et al, 2023 ). In vivo results show that a hypoxic microenvironment induces an upregulation of miR-210 leading to increased breast cancer progression by reducing the expression of E-cadherin protein in breast cancer stem cells, whereas the inhibition of this miRNA suppresses cell proliferation and consequent metastasis ( Tang et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…The individual expression levels of miRNAs were detected by the RT-qPCR technique using the QuantStudio 3 equipment (Thermo Fisher Scientific, Massachusetts, United States) and the TaqMan Fast Advanced Master Mix assay (Thermo Fisher Scientific, Massachusetts, United States), under standard cycling conditions, according to the manufacturer’s recommendations. The amplification reaction consisted of 50 ng of cDNA, TaqMan Master Mix, and a specific probe for each miRNA: hsa-miR-1-3p (ID 002222, miRbase MI0000651) ( Liu et al, 2017 ; Wu, 2018 ; Peng et al, 2020 ; Chen et al, 2021 ); hsa-miR-16-5p (ID 000391, miRbase MI0000070) ( Qu et al, 2017 ; Tommasi et al, 2021 ; Wang et al, 2021 ); hsa-miR-21-5p (ID 000397, miRbase MI0000077) ( Wu et al, 2012 ; Li et al, 2016 ; Chen et al, 2019 ; Wang et al, 2019 ); hsa-miR-210-3p (ID 000512, miRbase MI0000286) ( Evangelista et al, 2021 ; Khalilian et al, 2023 ; Santana et al, 2023 ); hsa-miR-195-5p (ID 000494, miRbase MI0000489) ( Singh et al, 2015 ; McAnena et al, 2019 ; Chen et al, 2022 ); hsa-miR-10b-5p (ID 002218, miRbase MI0000267) ( Han et al, 2014 ; Nassar et al, 2014 ; Zhang et al, 2018 ; Raval et al, 2022 ), and hsa-miR-34a-5p (ID 000426, MI0000268) ( Peurala et al, 2011 ; Misso et al, 2014 ; Zaleski et al, 2018 ; Chen et al, 2021 ). Assay ID for TaqMan MicroRNA Assay, Thermo Fisher Scientific.…”

Section: Methodsmentioning

confidence: 99%

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MicroRNA as a promising molecular biomarker in the diagnosis of breast cancer

Miranda,

Slaibi-Filho,

Calasans dos Santos

et al. 2024

Front. Mol. Biosci.

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Introduction: Breast cancer represents the most prevalent malignancy among women. Recent advancements in translational research have focused on the identification of novel biomarkers capable of providing valuable insights into patient outcomes. Furthermore, comprehensive investigations aimed at discovering novel miRNAs, unraveling their biological functions, and deciphering their target genes have significantly contributed to our understanding of the roles miRNAs play in tumorigenesis. Consequently, these investigations have facilitated the way for the development of miRNA-based approaches for breast cancer prognosis, diagnosis, and treatment. However, conducting a more extensive array of studies, particularly among diverse ethnic groups, is imperative to expand the scope of research and validate the significance of miRNAs. This study aimed to assess the expression patterns of circulating miRNAs in plasma as a prospective biomarker for breast cancer patients within a population primarily consisting of individuals from Black, Indigenous, and People of Color (BIPOC) communities.Methods: We evaluated 49 patients with breast cancer compared to 44 healthy women.Results and discussion: All miRNAs analyzed in the plasma of patients with breast cancer were downregulated. ROC curve analysis of miR-21 (AUC = 0.798, 95% CI: 0.682–0.914, p <0.0001), miR-1 (AUC = 0.742, 95% CI: 0.576–0.909, p = 0.004), miR-16 (AUC = 0.721, 95% CI: 0.581–0.861, p = 0.002) and miR-195 (AUC = 0.672, 95% CI: 0.553–0.792, p = 0.004) showed better diagnostic accuracy in discrimination of breast cancer patients in comparison with healthy women. miR-210, miR-21 showed the highest specificities values (97.3%, 94.1%, respectively). Following, miR-10b and miR-195 showed the highest sensitivity values (89.3%, and 77.8%, respectively). The panel with a combination of four miRNAs (miR-195 + miR-210 + miR-21 + miR-16) had an AUC of 0.898 (0.765–0.970), a sensitivity of 71.4%, and a specificity of 100.0%. Collectively, our results highlight the miRNA combination in panels drastically improves the results and showed high accuracy for the diagnosis of breast cancer displaying good sensitivity and specificity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNA as a promising molecular biomarker in the diagnosis of breast cancer

Miranda,

Slaibi-Filho,

Calasans dos Santos

et al. 2024

Front. Mol. Biosci.

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“…miR-210 exhibits pleiotropic effects and may regulate mitochondrial metabolism and apoptosis, among other cellular processes. It is worth noting that miRNAs can exert different effects depending on their cellular location and the specific signaling pathways they target [ 47 , 48 , 49 ]. The expression of miR-210 was not reduced in this study after treatment with the SPD derivatives ( Figure 5 ), which is consistent with the results obtained for the gene expression of Akt and CA IX .…”

Section: Discussionmentioning

confidence: 99%

The Mechanism of Anti-Tumor Activity of 6-Morpholino- and 6-Amino-9-Sulfonylpurine Derivatives on Human Leukemia Cells

Leventić,

Opačak-Bernardi,

Rastija

et al. 2023

Molecules

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The aim of this study was to explore the mechanism of antitumor effect of (E)-6-morpholino-9-(styrylsulfonyl)-9H-purine (6-Morpholino-SPD) and (E)-6-amino-9-(styrylsulfonyl)-9H-purine (6-Amino-SPD). The effects on apoptosis induction, mitochondrial potential, and accumulation of ROS in treated K562 cells were determined by flow cytometry. The RT-PCR method was used to measure the expression of Akt, CA IX, caspase 3, and cytochrome c genes, as well as selected miRNAs. Western blot analysis was used to determine the expression of Akt, cytochrome c, and caspase 3. The results demonstrate the potential of the tested derivatives as effective antitumor agents with apoptotic-inducing properties. In leukemic cells treated with 6-Amino-SPD, increased expression of caspase 3 and cytochrome c genes was observed, indicating involvement of the intrinsic mitochondrial pathway in the induction of apoptosis. Conversely, leukemic cells treated with 6-Morpholino-SPD showed reduced expression of these genes. The observed downregulation of miR-21 by 6-Morpholino-SPD may contribute to the induction of apoptosis and disruption of mitochondrial function. In addition, both derivatives exhibited increased expression of Akt and CA IX genes, suggesting activation of the Akt/HIF pathway. However, the exact mechanism and its relations to the observed overexpression of miR-210 need further investigation. The acceptable absorption and distribution properties predicted by ADMET analysis suggest favorable pharmacokinetic properties for these derivatives.

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“…This regulatory process includes the up-regulation of several specific hypoxia-inducible microRNAs, often referred to as “hypoxamiRs”, with miR-210 being the foremost among them 6,7 . Accordingly, miR-210 has been shown to play a role in many hypoxia-related diseases 10,11 , particularly in cardiovascular diseases 7,12 and cancer 8,11,13 . Nevertheless, miR-210 is not merely a passive participant in hypoxia 8 , and it continues to unveil novel roles and molecular functions, some of which are unrelated to hypoxic conditions.…”

Section: Introductionmentioning

confidence: 99%

miR-210 is essential to retinal homeostasis in fruit flies and mice

Colaianni,

Virga,

Tisi

et al. 2024

Preprint

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SummarymiR-210 is one of the most evolutionarily conserved microRNAs. Recent studies inDrosophila melanogasterhave unveiled that the absence of miR-210 leads to a progressive retinal degeneration characterized by the accumulation of lipid droplets and disruptions in lipid metabolism. Further investigation into lipid anabolism and catabolism revealed significant alterations in gene expression within these pathways. We provide the first morphological characterization of miR-210 KO mice retinas, highlighting a significant photoreceptor degeneration. While exploring potential parallels between miR-210 KO models in flies and mice, we examined mice lipid metabolism, circadian behaviour, and retinal transcriptome yet found no resemblances, suggesting divergent mechanisms of retinal degeneration between the two species. Simultaneously, analysis of the transcriptome in the brains of miR-210 KO flies revealed the potential existence of a shared upstream mechanism contributing to retinal degeneration in both fruit flies and mammals.

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A review on the role of miR-210 in human disorders

Cited by 12 publications

References 38 publications

MicroRNA as a promising molecular biomarker in the diagnosis of breast cancer

MicroRNA as a promising molecular biomarker in the diagnosis of breast cancer

The Mechanism of Anti-Tumor Activity of 6-Morpholino- and 6-Amino-9-Sulfonylpurine Derivatives on Human Leukemia Cells

miR-210 is essential to retinal homeostasis in fruit flies and mice

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