A Review on Molecular Tools of Microalgal Genetic Transformation and their Application for Overexpression of Different Genes

Rathod, Jayant Pralhad; Gade, R. M.; Rathod, Darasing R.; Dudhare, M. S.

doi:10.20546/ijcmas.2017.612.373

Cited by 15 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only few stramenopile microalgae can nowadays be transformed (Rathod et al, 2017). Moreover, in vivo subcellular targeting analyses of N. oceanica require 6-8 weeks from transformation to microscopy of liquid cultures (de Grahl and Rout, personal communication;de Grahl et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile Alga Nannochloropsis

Kechasov

Grahl

Endries

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile Alga Nannochloropsis

Kechasov

Grahl

Endries

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

“…Cell walled, Amphidinium sp, Symbiodinium microadriaticum and C. reinhardtii are also transformed using this technique. Although the transformation efficiency is much better but the health hazard caused by inhaling silicon while handling is one of the major drawback ( Rathod et al, 2017 ).…”

Section: Algal Transformation Methodsmentioning

confidence: 99%

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Sreenikethanam

Raj

Banu

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Microalgae are highly diverse photosynthetic organisms with higher growth rate and simple nutritional requirements. They are evolved with an efficiency to adapt to a wide range of environmental conditions, resulting in a variety of genetic diversity. Algae accounts for nearly half of global photosynthesis, which makes them a crucial player for CO2 sequestration. In addition, they have metabolic capacities to produce novel secondary metabolites of pharmaceutical, nutraceutical and industrial applications. Studies have explored the inherent metabolic capacities of microalgae with altered growth conditions for the production of primary and secondary metabolites. However, the production of the targeted metabolites at higher rates is not guaranteed just with the inherent genetic potentials. The strain improvement using genetic engineering is possible hope to overcome the conventional methods of culture condition improvements for metabolite synthesis. Although the advanced gene editing tools are available, the gene manipulation of microalgae remains relatively unexplored. Among the performed gene manipulations studies, most of them focus on primary metabolites with limited focus on secondary metabolite production. The targeted genes can be overexpressed to enhance the production of the desired metabolite or redesigning them using the synthetic biology. A mutant (KOR1) rich in carotenoid and lipid content was developed in a recent study employing mutational breeding in microalgae (Kato, Commun. Biol, 2021, 4, 450). There are lot of challenges in genetic engineering associated with large algal diversity but the numerous applications of secondary metabolites make this field of research very vital for the biotech industries. This review, summarise all the genetic engineering studies and their significance with respect to secondary metabolite production from microalgae. Further, current genetic engineering strategies, their limitations and future strategies are also discussed.

show abstract

“…This introduction cannot be established without causing a temporal permeabilization of the cell membrane or the cell wall of the receptor cell. Thus, integration of the exogenous DNA into the genome of the receptor cell mainly occurs by random recombination and can succeed with or without any external assistance [118]. Practically, the embrittlement of the cell membrane or the cell wall of the host cell is not the most exhausting stage of the genetic transformation procedure.…”

Section: Diatoms' Transformation Methodsmentioning

confidence: 99%

Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i> as a Model for Producing High-Added Value Molecules

Dhaouadi¹,

Awwad²,

Diamond³

et al. 2020

AJPS

View full text Add to dashboard Cite

With a world growing in population and nutritional needs, diatoms are considered nowadays as microalgae of a very important potential, thus they are exploited in several fields such as ecology, aquaculture, molecular farming, and pharma nutraceuticals. These coveted microalgae are characterized by their diversity, their high division rates, their complex life cycle, likewise their silicified cell walls named frustules. Thus, diatoms have been used for over a century after proving an efficient production of several molecules including Triacylglycerols (TAGs), H 2 , free fatty acids, vitamins, nutraceuticals, amino acids, proteins, terpenoids, alcohols and carbohydrates like starch, glycogen, and sucrose. Phaeodactylum tricornutum is the most promising diatom exploited to date, especially as a platform of pharmaceutical production. Herein, we expose diatoms' main features that allowed using them for molecular farming. This review exposes likewise, the metabolism and the post-translational modifications (PTMs) of diatoms as well as current tools and challenges for their molecular and metabolic engineering for a more efficient production of valuable molecules. The knowledge on the biology of the diatoms, the molecular tools, and the various transformation methods available demonstrate the potential in biotechnology of these photosynthetic microorganisms. The widely studied P. tricornutum, as a model organism, is a promising diatom for production of valuable metabolites, despite the challenges and issues related to cultivation.

show abstract

A Review on Molecular Tools of Microalgal Genetic Transformation and their Application for Overexpression of Different Genes

Cited by 15 publications

References 6 publications

Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile Alga Nannochloropsis

Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile Alga Nannochloropsis

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i> as a Model for Producing High-Added Value Molecules

Contact Info

Product

Resources

About

A Review on Molecular Tools of Microalgal Genetic Transformation and their Application for Overexpression of Different Genes

Cited by 15 publications

References 6 publications

Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile Alga Nannochloropsis

Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile Alga Nannochloropsis

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Diatoms’ Breakthroughs in Biotechnology: &lt;i&gt;Phaeodactylum tricornutum&lt;/i&gt; as a Model for Producing High-Added Value Molecules

Contact Info

Product

Resources

About

Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i> as a Model for Producing High-Added Value Molecules