A Review on Micropropagation Culture Method

Gupta, Nikita; Jain, Vidhi; Joseph, Merin Rosy; Devi, Siwani

doi:10.22270/ajprd.v8i1.653

Cited by 26 publications

(16 citation statements)

References 10 publications

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“…Since food insecurity is still a serious global challenge, it is imperative todevelop a direct shoot organogenesis protocol for crops of economic importance as these protocols enable us to produce large numbers of new high-quality plantlets in a relatively short time and space. Micropropagation is a low-cost technology, and plantlet derived from micropropagation helps in plants germplasm conservation (Gupta et al, 2020). In this study, micropropagation method was developed for two common varieties of taro in Kenya, that is, Dasheen and Purple Wild.…”

Section: Discussionmentioning

confidence: 99%

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

Kepue¹,

Mweu²,

Kariuki³

et al. 2021

Afr. J. Biotechnol.

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Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from portions of the corm and base of leaf petioles. This study optimized the 6benzylaminopurine (BAP) and indole-3-butyric acid (IBA) levels for a better shoot and root development. The highest shoot induction was observed in both varieties when Murashige & Skoog (MS) media was supplemented with 2 mg/L of BAP. Similarly, the maximum rooting response was achieved in both varieties on half-strength MS media supplemented with 0.5 mg/L of IBA. The study also revealed significant interactions between variety × BAP levels and variety × IBA levels (P<0.0001) for shoot and root development, respectively. This apical meristem-based micropropagation method developed in this study can be used for rapid multiplication of genetically clean planting materials for commercial uses and to establish taro transformation protocols.

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Section: Discussionmentioning

confidence: 99%

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

Kepue¹,

Mweu²,

Kariuki³

et al. 2021

Afr. J. Biotechnol.

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“…Micropropagation can be carried out using nodal explants, shoot tips, or leaf segments, which are immersed within a specific growth media. BAP (6‐benzylaminopurine) and NAA (naphthaleneacetic acid) are commonly used as regulators to promote shoot proliferation, thus increasing the enzyme production rate [44] …”

Section: Production Of Plant Proteasesmentioning

confidence: 99%

“…BAP (6benzylaminopurine) and NAA (naphthaleneacetic acid) are commonly used as regulators to promote shoot proliferation, thus increasing the enzyme production rate. [44] The costs associated to regulators compounds are crucial to scale up of micropropagation techniques to produce highquality roots. An optimized growth medium is required for each particular enzyme according to the explant types in order to achieve a feasible yield.…”

Section: Micropropagation and Somatic Embryogenesismentioning

confidence: 99%

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

2022

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An updated review of emerging plant proteases with potential biotechnological application is presented. Plant proteases show comparable or even greater performance than animal or microbial proteases for by‐product valorization through hydrolysis for, for example, cheese whey, bird feathers, collagen, keratinous materials, gelatin, fish protein, and soy protein. Active biopeptides can be obtained as high added value products, which have shown numerous beneficial effects on human health. Plant proteases can also be used for wastewater treatment. The production of new plant proteases is encouraged for the following advantages: low cost of isolation using simple procedures, remarkable stability over a wide range of operating conditions (temperature, pH, salinity, and organic solvents), substantial affinity to a broad variety of substrates, and possibility of immobilization. Vegetable proteases have enormous application potential for the valorization of industrial waste and its conversion into products with high added value through low‐cost processes.

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“…Plant micropropagation is one of the best alternatives for crop improvement and shows a special significance in multiplication through a vegetative approach. This minimizes the risk of pathogen accumulation and the slow proliferation rate in generative multiplication, which in turn, causes a loss in both crop quantity and quality [39][40][41][42]. Several studies have shown the positive impacts of AgNP augmentation for callus induction, shoot generation, and growth (Table 1).…”

Section: Silver-nanoparticles In Crop Improvementmentioning

confidence: 99%

Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

et al. 2022

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Plant tissue culture plays an important role in plant biotechnology due to its potential for massive production of improved crop varieties and high yield of important secondary metabolites. Several efforts have been made to ameliorate the effectiveness and production of plant tissue culture, using biotic and abiotic factors. Nowadays, the addition of nanoparticles as elicitors has, for instance, gained worldwide interest because of its success in microbial decontamination and enhancement of secondary metabolites. Nanoparticles are entities in the nanometric dimension range: they possess unique physicochemical properties. Among all nanoparticles, silver-nanoparticles (AgNPs) are well-known for their antimicrobial and hormetic effects, which in appropriate doses, led to the improvement of plant biomass as well as secondary metabolite accumulation. This review is focused on the evaluation of the integration of nanotechnology with plant tissue culture. The highlight is especially conveyed on secondary metabolite enhancement, effects on plant growth and biomass accumulation as well as their possible mechanism of action. In addition, some perspectives of the use of nanomaterials as potential therapeutic agents are also discussed. Thus, the information provided will be a good tool for future research in plant improvement and the large-scale production of important secondary metabolites. Elicitation of silver-nanoparticles, as well as nanomaterials, function as therapeutic agents for animal well-being is expected to play a major role in the process. However, nanosized supramolecular aggregates have received an increased resonance also in other fields of application such as animal welfare. Therefore, the concluding section of this contribution is dedicated to the description and possible potential and usage of different nanoparticles that have been the object of work and expertise also in our laboratories.

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A Review on Micropropagation Culture Method

Cited by 26 publications

References 10 publications

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

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