A Review on Electrochemical Microsensors for Ascorbic Acid Detection: Clinical, Pharmaceutical, and Food Safety Applications

Abstract

Nowadays, micro-sized sensors have become a hot topic in electroanalysis. Because of their excellent analytical features, microelectrodes are well-accepted tools for clinical, pharmaceutical, food safety, and environmental applications. In this brief review, we highlight the state-of-art electrochemical non-enzymatic microsensors for quantitative detection of ascorbic acid (also known as vitamin C). Ascorbic acid is a naturally occurring water-soluble organic compound with antioxidant properties and its quantitative determination in biological fluids, foods, cosmetics, etc., using electrochemical microsensors is of wide interest. Various electrochemical techniques have been applied to detect ascorbic acid with extremely high sensitivity, selectivity, reproducibility, and reliability, and apply to in vivo measurements. This review paper aims to give readers a clear view of advances in areas of electrode modification, successful strategies for signal amplification, and miniaturization techniques used in the electroanalytical devices for ascorbic acid. In conclusion, current challenges related to the microelectrodes design, and future perspectives are outlined.

In Situ Ratiometric Determination of Cerebral Ascorbic Acid after Ischemia Reperfusion

“…Figure B showed that there was a linear relationship in the concentration range of AA from 150 to 500 μM; the limit of detection (LOD) was calculated to be 9.3 μM. The linear range of AA detection well-covered the physiological concentration of AA in the living brain . Then, we further explored the stability of the CFE/CNT –COOH /PEDOT electrodes.…”

“…The linear range of AA detection well-covered the physiological concentration of AA in the living brain. 61 Then, we further explored the stability of the CFE/CNT −COOH /PEDOT electrodes. As demonstrated in Figure 3C, the CFE/ CNT −COOH /PEDOT electrode was so stable that the current decreased by only 4% after continuous DPV scanning for 300 cycles.…”

MnOx In Situ Growth-Induced Luminescence and Oxidase-Like Feature Bimodulation of CePO4:Tb Nanorods: Toward Ascorbic Acid-Related Bioanalysis in a “One-Stone-Two-Birds” Manner

“…AA plays an important role in many physiological processes and is associated with numerous diseases . Establishing reliable, sensitive, and selective assays to accurately quantify AA is significant for clinical diagnosis.…”

“…AA plays an important role in many physiological processes and is associated with numerous diseases. 47 Establishing reliable, sensitive, and selective assays to accurately quantify AA is significant for clinical diagnosis. Because of the unique PL and oxidase-like activity switching behaviors of CePO 4 :Tb@MnO x , which can be adjusted by the synergistic regulatory mechanism through specific redox reactions (KMnO 4 for oxidation and AA for reduction), a dual-mode colorimetric/fluorometric assay of AA was established.…”

Multiwalled Carbon Nanotubes-Modified Metallic Electrode Prepared Using Chemical Vapor Deposition as Sequential Injection Analysis Detector for Determination of Ascorbic Acid

“…Electrochemical techniques have advantages over other techniques such as spectrometry and chromatography in food, biological, pharmaceutical, and clinical applications. These include high precision and sensitivity, selectivity, cost effectiveness, defined reaction time, environmentally friendly, miniaturization, and minimum sample pretreatment [ 1 , 2 ]. Nanomaterials have been extensively used to develop advanced electrochemical sensors and biosensors for a wide range of applications [ 3 , 4 , 5 ].…”