A review on CRISPR/Cas-based epigenetic regulation in plants

Jogam, Phanikanth; Sandhya, Dulam; Alok, Anshu; Peddaboina, Venkataiah; Allini, Venkateswar Rao; Zhang, Baohong

doi:10.1016/j.ijbiomac.2022.08.182

Cited by 29 publications

(15 citation statements)

References 143 publications

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“…Application of multiplex genome editing would open the ways for the development of genome-edited crops engineered for tolerance against multiple traits in a single transformation event. Thus, it is predicted that genome editing will become the technology of choice ( Jogam et al., 2022 ). The lack of validated target for genome editing would be main obstruction in unlocking the CRISPR potential to develop stress tolerant crop plants.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

et al. 2023

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Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches and transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery of engineered nucleases as genetic scissors to carry out precise manipulation in crop stress-responsive genes and associated molecular network has paved the way for sustainable management of abiotic stress conditions. In this context, the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool has revolutionized due to its simplicity, accessibility, adaptability, flexibility, and wide applicability. This system has great potential to build up crop varieties with enhanced tolerance against abiotic stresses. In this review, we summarize the latest findings on understanding the mechanism of abiotic stress response in plants and the application of CRISPR/Cas-mediated gene-editing system towards enhanced tolerance to a multitude of stresses including drought, salinity, cold, heat, and heavy metals. We provide mechanistic insights on the CRISPR/Cas9-based genome editing technology. We also discuss applications of evolving genome editing techniques such as prime editing and base editing, mutant library production, transgene free and multiplexing to rapidly deliver modern crop cultivars adapted to abiotic stress conditions.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

et al. 2023

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“…Conventional methods can also be used to manipulate epigenetic remodelers for epigenome rewriting. Nevertheless, the CRISPR/Cas technology has greatly accelerated the advancement of epigenetic editing in bacteria [ 87 ], mammalian cells [ 88 ], plants [ 89 ], and fungi [ 90 ].…”

Section: Application Of Crispr/cas Systems In Fungal Genetic Engineeringmentioning

confidence: 99%

Advances and Challenges in CRISPR/Cas-Based Fungal Genome Engineering for Secondary Metabolite Production: A Review

Wang

Jin

et al. 2023

JoF

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Fungi represent an important source of bioactive secondary metabolites (SMs), which have wide applications in many fields, including medicine, agriculture, human health, and many other industries. The genes involved in SM biosynthesis are usually clustered adjacent to each other into a region known as a biosynthetic gene cluster (BGC). The recent advent of a diversity of genetic and genomic technologies has facilitated the identification of many cryptic or uncharacterized BGCs and their associated SMs. However, there are still many challenges that hamper the broader exploration of industrially important secondary metabolites. The recent advanced CRISPR/Cas system has revolutionized fungal genetic engineering and enabled the discovery of novel bioactive compounds. In this review, we firstly introduce fungal BGCs and their relationships with associated SMs, followed by a brief summary of the conventional strategies for fungal genetic engineering. Next, we introduce a range of state-of-the-art CRISPR/Cas-based tools that have been developed and review recent applications of these methods in fungi for research on the biosynthesis of SMs. Finally, the challenges and limitations of these CRISPR/Cas-based systems are discussed and directions for future research are proposed in order to expand their applications and improve efficiency for fungal genetic engineering.

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“…Using multiplex genome editing would allow for the development of genome-edited crops engineered for tolerance against multiple traits in a single transformation event. Therefore, it is expected that genome editing will become the technology of choice for developing desired genetically and epigenetically [ 215 , 216 ] biotech crops for different purposes particularly to address the food shortage problems as well as to fight climate change more effectively.…”

Section: Conclusion and Future Directionmentioning

confidence: 99%

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

Rahman

Zulfiqar

Raza

et al. 2022

Cells

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Environmental abiotic stresses challenge food security by depressing crop yields often exceeding 50% of their annual production. Different methods, including conventional as well as genomic-assisted breeding, mutagenesis, and genetic engineering have been utilized to enhance stress resilience in several crop species. Plant breeding has been partly successful in developing crop varieties against abiotic stresses owning to the complex genetics of the traits as well as the narrow genetic base in the germplasm. Irrespective of the fact that genetic engineering can transfer gene(s) from any organism(s), transgenic crops have become controversial mainly due to the potential risk of transgene-outcrossing. Consequently, the cultivation of transgenic crops is banned in certain countries, particularly in European countries. In this scenario, the discovery of the CRISPR tool provides a platform for producing transgene-free genetically edited plants—similar to the mutagenized crops that are not extensively regulated such as genetically modified organisms (GMOs). Thus, the genome-edited plants without a transgene would likely go into the field without any restriction. Here, we focused on the deployment of CRISPR for the successful development of abiotic stress-tolerant crop plants for sustaining crop productivity under changing environments.

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A review on CRISPR/Cas-based epigenetic regulation in plants

Cited by 29 publications

References 143 publications

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Advances and Challenges in CRISPR/Cas-Based Fungal Genome Engineering for Secondary Metabolite Production: A Review

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

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