A review on contemporary approaches in enhancing the innate lipid content of yeast cell

Sundaramahalingam, M.A.; Sivashanmugam, P.; Banu, J. Rajesh; Ashokkumar, Muthupandian

doi:10.1016/j.chemosphere.2022.133616

Cited by 19 publications

(2 citation statements)

References 134 publications

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“…The ability to convert carbohydrates to lipids, as a result, makes them advantageous for the production of biofuels [ 42 , 72 ]. Certain yeast species can accumulate a wide variety of lipids between 20 and 80% (w/w) of their dry weight [ 73 ]. Despite the fact that the process is similar in most species, the mechanism by which these microorganisms accumulate lipids is not well understood.…”

Section: Oleaginous Yeasts As An Alternative To Algae For Biodiesel P...mentioning

confidence: 99%

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Ali

Al-Tohamy

Mohamed

et al. 2022

Biotechnol Biofuels

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Biodiesel is a renewable fuel that can be produced from a range of organic and renewable feedstock including fresh or vegetable oils, animal fats, and oilseed plants. In recent years, the lignin-based aromatic wastes, such as various aromatic waste polymers from agriculture, or organic dye wastewater from textile industry, have attracted much attention in academia, which can be uniquely selected as a potential renewable feedstock for biodiesel product converted by yeast cell factory technology. This current investigation indicated that the highest percentage of lipid accumulation can be achieved as high as 47.25% by an oleaginous yeast strain, Meyerozyma caribbica SSA1654, isolated from a wood-feeding termite gut system, where its synthetic oil conversion ability can reach up to 0.08 (g/l/h) and the fatty acid composition in yeast cells represents over 95% of total fatty acids that are similar to that of vegetable oils. Clearly, the use of oleaginous yeasts, isolated from wood-feeding termites, for synthesizing lipids from aromatics is a clean, efficient, and competitive path to achieve "a sustainable development" towards biodiesel production. However, the lacking of potent oleaginous yeasts to transform lipids from various aromatics, and an unknown metabolic regulation mechanism presented in the natural oleaginous yeast cells are the fundamental challenge we have to face for a potential cell factory development. Under this scope, this review has proposed a novel concept and approach strategy in utilization of oleaginous yeasts as the cell factory to convert aromatic wastes to lipids as the substrate for biodiesel transformation. Therefore, screening robust oleaginous yeast strain(s) from wood-feeding termite gut system with a set of the desirable specific tolerance characteristics is essential. In addition, to reconstruct a desirable metabolic pathway/network to maximize the lipid transformation and accumulation rate from the aromatic wastes with the applications of various “omics” technologies or a synthetic biology approach, where the work agenda will also include to analyze the genome characteristics, to develop a new base mutation gene editing technology, as well as to clarify the influence of the insertion position of aromatic compounds and other biosynthetic pathways in the industrial chassis genome on the expressional level and genome stability. With these unique designs running with a set of the advanced biotech approaches, a novel metabolic pathway using robust oleaginous yeast developed as a cell factory concept can be potentially constructed, integrated and optimized, suggesting that the hypothesis we proposed in utilizing aromatic wastes as a feedstock towards biodiesel product is technically promising and potentially applicable in the near future.

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Section: Oleaginous Yeasts As An Alternative To Algae For Biodiesel P...mentioning

confidence: 99%

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Ali

Al-Tohamy

Mohamed

et al. 2022

Biotechnol Biofuels

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“…All the yeasts reported in Figures 1 and 2 can produce intracellular lipids through the degradation of various substrates used as carbon sources and generate these compounds in different proportions. The lipids produced need to be extracted from the yeast biomass and then transformed into biodiesel through chemical reactions [71].…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production Processes with Yeast: A Sustainable Approach

Sánchez-Solís,

Lobato-Calleros,

Moreno-Terrazas

et al. 2024

Energies

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In recent years, renewable sources of energy have been sought due to the environmental impacts associated with fossil fuels, such as greenhouse gas emissions into the atmosphere. A promising alternative is biodiesel, particularly when obtained using yeast, as they offer certain advantages over other microorganisms due to their resilience to grow in various conditions, short reproduction times, and lower susceptibility to bacterial infections because they thrive at lower pH levels and have the ability to utilize a wide variety of substrates. Furthermore, biodiesel produced with yeast is composed of methyl ester fatty acids (FAME), providing it with good quality and performance in internal combustion engines, resulting in reduced greenhouse gas emissions compared to conventional diesel. The production of biodiesel using yeast involves six general stages, which offer various methodological alternatives with different degrees of sustainability. The objective of this review is to assess the sustainability degree of various methodologies employed in each of the stages of yeast-based biodiesel production through environmental and economic sustainability indicators.

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Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies

Hassane,

Eldiehy,

Saha

et al. 2024

Arch Microbiol

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A review on contemporary approaches in enhancing the innate lipid content of yeast cell

Cited by 19 publications

References 134 publications

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Biodiesel Production Processes with Yeast: A Sustainable Approach

Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies

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