A review on cationic lipids with different linkers for gene delivery

Zhi, Defu; Bai, Yuchao; Yang, Jian; Cui, Shaohui; Zhao, Yinan; Chen, Huiying; Zhang, Shubiao

doi:10.1016/j.cis.2017.12.006

Cited by 119 publications

(96 citation statements)

References 207 publications

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“…Furthermore, while cationic lipids bearing ether bonds result in better transfection, they are non-biodegradable and lead to higher cytotoxicity. Conversely, cationic lipids including ester linkers are prone to biodegradation but are less cytotoxic [182].…”

Section: Cationic Liposomesmentioning

confidence: 99%

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

et al. 2020

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Bone-related injury and disease constitute a significant global burden both socially and economically. Current treatments have many limitations and thus the development of new approaches for bone-related conditions is imperative. Gene therapy is an emerging approach for effective bone repair and regeneration, with notable interest in the use of RNA interference (RNAi) systems to regulate gene expression in the bone microenvironment. Calcium phosphate nanoparticles represent promising materials for use as non-viral vectors for gene therapy in bone tissue engineering applications due to their many favorable properties, including biocompatibility, osteoinductivity, osteoconductivity, and strong affinity for binding to nucleic acids. However, low transfection rates present a significant barrier to their clinical use. This article reviews the benefits of calcium phosphate nanoparticles for RNAi delivery and highlights the role of surface functionalization in increasing calcium phosphate nanoparticles stability, improving cellular uptake and increasing transfection efficiency. Currently, the underlying mechanistic principles relating to these systems and their interplay during in vivo bone formation is not wholly understood. Furthermore, the optimal microRNA targets for particular bone tissue regeneration applications are still unclear. Therefore, further research is required in order to achieve the optimal calcium phosphate nanoparticles-based systems for RNAi delivery for bone tissue regeneration.

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Section: Cationic Liposomesmentioning

confidence: 99%

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

et al. 2020

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“…The stability of phospholipid in serum that contained in liposomes might affect DNA protection ability more than the effect of central core structure. However, structure-activity relationships of changing central core structure was enigmatical [10].…”

Section: Resultsmentioning

confidence: 99%

Influence of serum on DNA protection ability and transfection efficiency of cationic lipid-based nanoparticles for gene delivery

et al. 2018

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Cationic lipid-based nanoparticulate systems are delivery systems that has been widely used in pharmaceutical field including gene delivery. There are many barriers obstructing genetic materials and their delivery systems to reach the target. Serum is one of the imperative factor that should be investigated. Therefore, the aim of this study was to examine the effect of serum on DNA protection ability of spermine-liposomes and niosomes by evaluating the percentage of transfection efficiency in Hela cell and observing the DNA degradation band using agarose gel electrophoresis in the presence of serum. The results showed that the percentage of transfection efficiency of spermine-liposomes was dramatically decreased when serum is presented (p< 0.05). In contrast, whether or not the serum is presented, the spermine-niosomes showed no significant difference in transfection efficiency. Concisely, liposomes could slightly protect DNA from DNase in the serum, whereas, niosomes had potential ability to protect DNA from the enzymes in serum. This result revealed an advantage of the cationic niosomes system as a gene carrier over the cationic liposomes.

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“…Mainly for its promising applications in health, LAAs are an important area of further studies and manipulation to achieve better quality products. Further research areas appear to be important: (1) Develop suite bioengineered biocatalysts to perform LAAs biosynthesis; (2) Protein engineering strategies can be applied to create novel biocatalysts; (3) Develop and optimize immobilization strategies for new biocatalysts; (4) Since the gemini and the substrates have common parts and polarities the purification process through a quick an inexpensive process will be challenging; (5) Understanding the interaction between LAAs and DNA for better product selectivity; (6) Test the efficacy and safety of LAAs as gene and drug delivery systems; and, (7) These tests must include (i) cytotoxicity evaluation, (ii) characterization of the interaction of the gemini LAAs with bioactive molecules, like nucleic acids and peptides, (iii) its action on the target cells, and (iv) optimization of the quantities and proportions of LAAs.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, cationic gemini surfactants have attracted special attention as efficient transfection agents in vitro and promising alternatives to viral vectors in gene therapy [4][5][6]. Successful gene therapy crucially depends on the development of effective vectors, especially for the safe introduction of the selected gene into living cells.…”

Section: Introductionmentioning

confidence: 99%

Lipoaminoacids Enzyme-Based Production and Application as Gene Delivery Vectors

et al. 2019

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Biosurfactant compounds have been studied in many applications, including biomedical, food, cosmetic, agriculture, and bioremediation areas, mainly due to their low toxicity, high biodegradability, and multifunctionality. Among biosurfactants, the lipoplexes of lipoaminoacids play a key role in medical and pharmaceutical fields. Lipoaminoacids (LAAs) are amino acid-based surfactants that are obtained from the condensation reaction of natural origin amino acids with fatty acids or fatty acid derivatives. LAA can be produced by biocatalysis as an alternative to chemical synthesis and thus become very attractive from both the biomedical and the environmental perspectives. Gemini LAAs, which are made of two hydrophobic chains and two amino acid head groups per molecule and linked by a spacer at the level of the amino acid residues, are promising candidates as both drug and gene delivery and protein disassembly agents. Gemini LAA usually show lower critical micelle concentration, interact more efficiently with proteins, and are better solubilising agents for hydrophobic drugs when compared to their monomeric counterparts due to their dimeric structure. A clinically relevant human gene therapy vector must overcome or avoid detect and silence foreign or misplaced DNA whilst delivering sustained levels of therapeutic gene product. Many non-viral DNA vectors trigger these defence mechanisms, being subsequently destroyed or rendered silent. The development of safe and persistently expressing DNA vectors is a crucial prerequisite for a successful clinical application, and it one of the main strategic tasks of non-viral gene therapy research.

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A review on cationic lipids with different linkers for gene delivery

Cited by 119 publications

References 207 publications

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

Influence of serum on DNA protection ability and transfection efficiency of cationic lipid-based nanoparticles for gene delivery

Lipoaminoacids Enzyme-Based Production and Application as Gene Delivery Vectors

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