Abstract:Pathogens are one of the leading causes of death globally. Early detection of pathogens seems to be necessary. Detection of bacteria and harmful viruses like severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a fast manner is very critical for early diagnosis of infections to prevent spreading. Current tools for the detection of pathogens are challenging in terms of specificity, rapidity, and simplicity. For this purpose, biosensors have shown advances in rapid diagnosis, high sensitivity, and sel… Show more
“…The development of electrochemical and optical sensors based on nanomaterials and polymers has arisen from the integration of progress in the fields of nanoscience, materials science, and engineering [51,52]. Over the years, there has been significant progress in the advancement of electrochemical sensors relying on nanomaterials and polymers.…”
Section: Historical Overview Of Nanomaterials and Polymerbased Electr...mentioning
A critical step in the process for preventing and identifying emergencies relating to health, safety, and welfare is the testing and quick diagnosis of microbial pathogens. Due to the fast spread of waterborne and food borne infections in society and the high costs associated with them, pathogen identification has emerged as one of the most difficult parts of the water and food sectors. Since the turn of the century, pathogens have demonstrated enormous epidemiological and pandemic potential. The emergence and dissemination of a novel virus with pandemic potential endanger the livelihoods and well-being of individuals worldwide. The severe acute respiratory syndrome-coronavirus-2 (SARS-COV-2) coronavirus pandemic has propagated to almost every country on Earth and has had a considerable negative influence on economies and communities. Despite improvements in identification techniques for viral diseases, all nations must now execute biosensing in a speedy, sensitive, focused, and consistent manner in order to address pressing global issues. Hence, in this review, we have critically summarised the recent advancement of electrochemical as well as optical biosensors for the monitoring of SARS-COV-2 and various pathogens. Then, we began by providing a technical overview of cutting-edge strategies utilised to combat diseases and emergencies for it, including the utilisation of point-of-care technology (POCT), artificial intelligence (AI), and the internet of medical things (IoMT). This review article explores the integration of POC, IoMT, and AI technologies in the context of personal healthcare, focusing on their potential to expedite the diagnosis and treatment of medical conditions, ultimately leading to improved patient outcomes. Subsequently, the notion and execution of multiplex testing are presented to enhance the comprehension of detecting multiple analytes. Finally, conclusions and future directions have been presented.
“…The development of electrochemical and optical sensors based on nanomaterials and polymers has arisen from the integration of progress in the fields of nanoscience, materials science, and engineering [51,52]. Over the years, there has been significant progress in the advancement of electrochemical sensors relying on nanomaterials and polymers.…”
Section: Historical Overview Of Nanomaterials and Polymerbased Electr...mentioning
A critical step in the process for preventing and identifying emergencies relating to health, safety, and welfare is the testing and quick diagnosis of microbial pathogens. Due to the fast spread of waterborne and food borne infections in society and the high costs associated with them, pathogen identification has emerged as one of the most difficult parts of the water and food sectors. Since the turn of the century, pathogens have demonstrated enormous epidemiological and pandemic potential. The emergence and dissemination of a novel virus with pandemic potential endanger the livelihoods and well-being of individuals worldwide. The severe acute respiratory syndrome-coronavirus-2 (SARS-COV-2) coronavirus pandemic has propagated to almost every country on Earth and has had a considerable negative influence on economies and communities. Despite improvements in identification techniques for viral diseases, all nations must now execute biosensing in a speedy, sensitive, focused, and consistent manner in order to address pressing global issues. Hence, in this review, we have critically summarised the recent advancement of electrochemical as well as optical biosensors for the monitoring of SARS-COV-2 and various pathogens. Then, we began by providing a technical overview of cutting-edge strategies utilised to combat diseases and emergencies for it, including the utilisation of point-of-care technology (POCT), artificial intelligence (AI), and the internet of medical things (IoMT). This review article explores the integration of POC, IoMT, and AI technologies in the context of personal healthcare, focusing on their potential to expedite the diagnosis and treatment of medical conditions, ultimately leading to improved patient outcomes. Subsequently, the notion and execution of multiplex testing are presented to enhance the comprehension of detecting multiple analytes. Finally, conclusions and future directions have been presented.
“…Nowadays, AuNPs are used for the ultrasensitive detection of varied targets [29] such as enzymes [30,31], proteins [32], DNA [33,34], cells [35], viruses [36][37][38][39][40][41], and bacteria [42,43]. Moreover, promising therapeutic approaches have just begun using AuNPs as antiviral agents capable of trapping viruses [44][45][46][47], in anticoagulant therapy [48], in photothermal therapy for breast cancer [49], and in the treatment of inflammatory bowel disease [50].…”
Since the outbreak of the pandemic respiratory virus SARS-CoV-2 (COVID-19), academic communities and governments/private companies have used several detection techniques based on gold nanoparticles (AuNPs). In this emergency context, colloidal AuNPs are highly valuable easy-to-synthesize biocompatible materials that can be used for different functionalization strategies and rapid viral immunodiagnosis. In this review, the latest multidisciplinary developments in the bioconjugation of AuNPs for the detection of SARS-CoV-2 virus and its proteins in (spiked) real samples are discussed for the first time, with reference to the optimal parameters provided by three approaches: one theoretical, via computational prediction, and two experimental, using dry and wet chemistry based on single/multistep protocols. Overall, to achieve high specificity and low detection limits for the target viral biomolecules, optimal running buffers for bioreagent dilutions and nanostructure washes should be validated before conducting optical, electrochemical, and acoustic biosensing investigations. Indeed, there is plenty of room for improvement in using gold nanomaterials as stable platforms for ultrasensitive and simultaneous “in vitro” detection by the untrained public of the whole SARS-CoV-2 virus, its proteins, and specific developed IgA/IgM/IgG antibodies (Ab) in bodily fluids. Hence, the lateral flow assay (LFA) approach is a quick and judicious solution to combating the pandemic. In this context, the author classifies LFAs according to four generations to guide readers in the future development of multifunctional biosensing platforms. Undoubtedly, the LFA kit market will continue to improve, adapting researchers’ multidetection platforms for smartphones with easy-to-analyze results, and establishing user-friendly tools for more effective preventive and medical treatments.
“…Additionally, metallic Au is well-known for its chemical inertness as well as its good thermal and electrical conductivity; thus, it has been utilised as bulk electrodes for electrochemical detection of arsenic [14,15]. Gold nanoparticles (AuNPs) can also be utilised for the detection of cyanide [16], uric acid [17], tetracycline [18], dopamine and acetaminophen [19], pesticide [20], cancer cells [21] or harmful pathogens [22].…”
In this study, gold nanoelectrodes were fabricated via electrical deposition of gold nanoparticles (NPs) onto FTO electrodes using cyclic voltammetry (CV) in 1 M H3PO4 solution at a scan rate of 100 mV.s−1 in the potential range of 0–1.4 V and −0.5–0.6 V. The fabricated FTO/AuNP electrodes were characterised by UV–vis, scanning electron microscope, energy-dispersive X-ray spectroscopy, as well as CV and linear sweep voltammetry; the presence of gold on the electrode surface and its electrochemical properties were confirmed towards hydroquinone. The electrodes with the best electrochemical properties were chosen for arsenic(III) determination. The fabricated FTO/AuNP electrodes in the potential range of 0–1.4 V exhibited the high sensitivity with limit of detection (LOD) of 3.04 ppb and limit of quantitation (LOQ) of 9.23 ppb, whereas the FTO/AuNP electrode fabricated in the potential range of −0.5 V–0.6 V displayed the enhancement sensitivity with LOD = 0.623 ppb and LOQ = 1.89 ppb.
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