A review of traditional and emerging methods to characterize lipid–protein interactions in biological membranes

Hsia, Chih‐Yun; Richards, Mark J.; Daniel, Susan

doi:10.1039/c5ay00599j

Cited by 27 publications

(29 citation statements)

References 182 publications

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“…Mobility of protein receptors, particularly those with transmembrane domains, can be a challenge in these platforms if the water gap is insufficient for limiting interaction between the protein and the glass support. To overcome this challenge, various cushions, such as polyethylene glycol (PEG) [52], bovine serum albumin, dextran [33,57], or polyelectrolyte brushes [54], have been placed between the bilayer and the supporting surface to improve protein mobility [58].…”

Section: Biomimetic Platformsmentioning

confidence: 99%

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms

Nathan

Daniel

2019

Advances in Experimental Medicine and Biology

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The most widely-used assays for studying viral entry, including infectivity, cofloatation, and cell-cell fusion assays, yield functional information but provide low resolution of individual entry steps. Structural characterization provides highresolution conformational information, but on its own is unable to address the functional significance of these conformations. Single virion tracking microscopy techniques provide more detail on the intermediate entry steps than infection assays and more functional information than structural methods, bridging the gap between these methods. In addition, single virion approaches also provide dynamic information about the kinetics of entry processes. This chapter reviews single virion tracking techniques and describes how they can be applied to study specific virus entry steps. These techniques provide information complementary to traditional ensemble approaches. Single virion techniques may either probe virion behavior in live cells or in biomimetic platforms. Synthesizing information from ensemble, structural, and single virion techniques ultimately yields a more complete understanding of the viral entry process than can be achieved by any single method alone.

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Section: Biomimetic Platformsmentioning

confidence: 99%

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms

Nathan

Daniel

2019

Advances in Experimental Medicine and Biology

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“…There are various methods to visualize membrane curvatures in situ or in reconstituted systems such as X-ray crystallography [48,49], nuclear magnetic resonance spectroscopy (NMR) [50,51], fluorescence microscopy [52,53], and electron microscopy (EM) [54,55]. Use of these techniques provides an opportunity for scientists to decipher vast amounts of information about the molecular machinery underlying the membrane shape transformations at high resolution.…”

Section: Introductionmentioning

confidence: 99%

Modeling Membrane Curvature Generation due to Membrane–Protein Interactions

Alimohamadi

Rangamani

2018

Biomolecules

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To alter and adjust the shape of the plasma membrane, cells harness various mechanisms of curvature generation. Many of these curvature generation mechanisms rely on the interactions between peripheral membrane proteins, integral membrane proteins, and lipids in the bilayer membrane. Mathematical and computational modeling of membrane curvature generation has provided great insights into the physics underlying these processes. However, one of the challenges in modeling these processes is identifying the suitable constitutive relationships that describe the membrane free energy including protein distribution and curvature generation capability. Here, we review some of the commonly used continuum elastic membrane models that have been developed for this purpose and discuss their applications. Finally, we address some fundamental challenges that future theoretical methods need to overcome to push the boundaries of current model applications.

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“…Membrane proteins represent approximately 25% of the human proteome, and they constitute more than 60% of current drug targets, due to their accessibility to extracellular molecules and roles in disease. [1][2][3][4] Strategies for the study of membrane protein interactions, and indeed interactions between proteins and membranes, 5 are therefore increasingly important to further our understanding of biomolecular function, both in fundamental research and drug discovery applications. However, membrane proteins are particularly challenging to study because they have large hydrophobic surfaces that can lead to misfolding or aggregation in aqueous solutions, as well as the low yield, solubility and stability of sample preparations.…”

Section: Introductionmentioning

confidence: 99%