A review of the roles of keratinocyte‐derived cytokines and chemokines in the pathogenesis of atopic dermatitis in humans and dogs

Asahina, Ryota; Maeda, Sadatoshi

doi:10.1111/vde.12351

Cited by 45 publications

(25 citation statements)

References 145 publications

(307 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interesting data, to increase the understanding of the beneficial effects of lactobacilli lysates on skin cells, were also suggested by the analysis of pro-inflammatory mediators performed by ELISA assays. Notably, keratinocytes produce various soluble factors such as chemokines and cytokines, which induce inflammation in response to a variety of stimuli, such as mechanical injury, allergens and bacteria 29 . The data obtained showed that all the LAB significantly reduced the release of the pro-inflammatory IL-8 from keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes

Brandi

Cheri

Manfredi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogenic effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders. Abbreviations LAB Lactic acid bacteria TNF-α Tumor necrosis factor-alpha IFN-γ Interferon-gamma PBS Phosphate buffered saline DMEM Dulbecco's modified Eagle's medium MCP-1 Monocyte chemoattractant protein-1 RANTES Regulated on activation, normal T cell expressed and secreted LC-MS/MS Liquid chromatography-tandem mass spectrometry SWATH Sequential window acquisition of all theoretical mass spectra The skin, considered the largest organ of the body, is involved in a variety of functions and acts primarily as a protective barrier preventing the entry of potential pathogens. In particular, skin homeostasis is regulated by microorganisms, the so called skin microbiota, which act on keratinocytes and on their cytokine release, ensuring

show abstract

Section: Discussionmentioning

confidence: 99%

Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes

Brandi

Cheri

Manfredi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Keratinocytes are the main cells that compose the stratum corneum and play a key role in AD pathogenesis. When keratinocytes are damaged by repetitive mechanical stimulations such as scratching behavior, various keratinocyte-derived cytokines and chemokines are secreted to promote inflammatory skin disease progression ( Asahina and Maeda, 2017 ). These secreted factors also cause additional responses by recruiting immune cells such as neutrophils, monocytes, and T cells to AD inflammatory skin lesions ( Giustizieri et al., 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Chijabyukpi-Tang Inhibits Pro-Inflammatory Cytokines and Chemokines via the Nrf2/HO-1 Signaling Pathway in TNF-α/IFN-γ-Stimulated HaCaT Cells and Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice

Lee

Lim

et al. 2020

Front. Pharmacol.

View full text Add to dashboard Cite

Chijabyukpi-tang (CBT) is an oriental herbal formula consisting of three herbs (Gardeniae Fructus (Gardenia jasminoides J.Ellis.), Phellodendri Cortex (Phellodendron amurense Rupr.), Glycyrrhizae Radix (Glycyrrhiza uralensis Fisch. ex DC.) at the ratio of 2: 2: 1. CBT has traditionally been used to treat eczema with inflammation in Northeast Asia. The components of CBT have been shown to have anti-inflammatory and anti-oxidant properties, but the exact role and mechanism of CBT on atopic dermatitis (AD) remain unclear. In this study, we investigated the anti-inflammatory effect and mechanism of CBT in the HaCaT human keratinocyte cell line and investigated the anti-atopic effect in mice models of atopic dermatitis-like skin lesions. In the tumor necrosis factor alpha (TNF)-a/ interferon (IFN)-g-stimulated HaCaT cells, CBT inhibited the production of proinflammatory cytokines and chemokines and elevated the nuclear translocation of NF-E2 p45 related factors 2 (Nrf2) and subsequent production of heme oxygenase-1 (HO-1). CBT improved the symptoms of atopic dermatitis-like lesions in 2,4-dinitrochlorobenzene (DNCB)-treated mice by suppressing the levels of serum immunoglobulin E (IgE), and various pro-inflammatory cytokines and chemokines. The improvement effect of CBT on atopic dermatitis-like lesions can be predicted to be due to increased Nrf2 and HO-1 gene expression. These results suggest that CBT is an herbal medicine with the potential for use as a therapeutic agent for inflammatory skin diseases such as atopic dermatitis.

show abstract

“…Of these cytokines, TSLP is produced mainly by keratinocytes and plays a critical role in the pathogenesis of AD, acting as a master regulator of allergic inflammation by promoting the differentiation of Th cells into Th2 cells either directly or via interactions with dendritic cells [4][5][6][7][8][9]. TSLP-mediated Th2 responses lead to the secretion of cytokines, including IL-4, IL-5, and IL-13, resulting in B-cell activation, which in turn induces an increase in immunoglobulin E (IgE) levels and the number of eosinophils [10,11].…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rh2 Ameliorates Atopic Dermatitis in NC/Nga Mice by Suppressing NF-kappaB-Mediated Thymic Stromal Lymphopoietin Expression and T Helper Type 2 Differentiation

Park

Lee

et al. 2019

IJMS

View full text Add to dashboard Cite

Ginsenosides are known to have various highly pharmacological activities, such as anti-cancer and anti-inflammatory effects. However, the search for the most effective ginsenosides against the pathogenesis of atopic dermatitis (AD) and the study of the effects of ginsenosides on specific cytokines involved in AD remain unclear. In this study, ginsenoside Rh2 was shown to exert the most effective anti-inflammatory action on thymic stromal lymphopoietin (TSLP) and interleukin 8 in tumor necrosis factor-alpha and polyinosinic: polycytidylic acid induced normal human keratinocytes by inhibiting proinflammatory cytokines at both protein and transcriptional levels. Concomitantly, Rh2 also efficiently alleviated 2,4-dinitrochlorobenzene-induced AD-like skin symptoms when applied topically, including suppression of immune cell infiltration, cytokine expression, and serum immunoglobulin E levels in NC/Nga mice. In line with the in vitro results, Rh2 inhibited TSLP levels in AD mice via regulation of an underlying mechanism involving the nuclear factor κB pathways. In addition, in regard to immune cells, we showed that Rh2 suppressed not only the expression of TSLP but the differentiation of naïve CD4+ T-cells into T helper type 2 cells and their effector function in vitro. Collectively, our results indicated that Rh2 might be considered as a good therapeutic candidate for the alternative treatment of AD.

show abstract

A review of the roles of keratinocyte‐derived cytokines and chemokines in the pathogenesis of atopic dermatitis in humans and dogs

Cited by 45 publications

References 145 publications

Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes

Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes

Chijabyukpi-Tang Inhibits Pro-Inflammatory Cytokines and Chemokines via the Nrf2/HO-1 Signaling Pathway in TNF-α/IFN-γ-Stimulated HaCaT Cells and Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice

Ginsenoside Rh2 Ameliorates Atopic Dermatitis in NC/Nga Mice by Suppressing NF-kappaB-Mediated Thymic Stromal Lymphopoietin Expression and T Helper Type 2 Differentiation

Contact Info

Product

Resources

About