A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease transmission in ET practices

Bielański, A.

doi:10.1016/j.theriogenology.2011.07.043

Cited by 112 publications

(99 citation statements)

References 85 publications

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“…The high risk of contamination because of the direct contact of oocytes/embryos with liquid nitrogen [153] using open carriers raises the need to develop safety strategies to reduce the risk of contamination. Recently, satisfactory results obtained using various types of "closed" systems [22,154] have allowed the transition to closed and more safe vitrification systems.…”

Section: Use Of Aseptic Technologiesmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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Section: Use Of Aseptic Technologiesmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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“…Although the question of contamination by bacteria, viruses or fungi during cooling or storage in LN 2 is being debated, the mere probability of contamination raises safety concerns (Bielanski and Vajta, 2009;Bielanski, 2012;Cobo et al, 2012). For this reason, great effort is taken to at least minimize the risk of contamination for the embryos during the vitrification process and when they are directly plunged into LN 2 .…”

Section: Introductionmentioning

confidence: 99%

Blastocyst transfer after aseptic vitrification of zygotes: an approach to overcome an impaired uterine environment

Vanderzwalmen

Zech

Ectors

et al. 2012

Reproductive BioMedicine Online

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(B Wirleitner).Pierre Vanderzwalmen, bio-engineer, entered the field of embryology in 1978 at the Veterinary Faculty of the University of Liege, Belgium. He is currently working as scientific co-ordinator at the IVF centres of Professor Zech in Austria, and at the CHIREC IVF-Institute of Professor Lejeune in Braine l'alleud-Brussels. His scientific investigations are focused on the selection of spermatozoa, embryo culture conditions, embryo development and vitrification of oocytes and embryos.Abstract In some IVF cycles, no fresh embryo transfer in the stimulated cycle is advisable. The cryopreservation of zygotes and the transfer of blastocysts in a cryo-embryo transfer is an option to circumvent an inadequate uterine environment due to risk of ovarian hyperstimulation syndrome, inappropriate endometrium build up, endometrial polyps or uterine myomas. For this strategy, highly secure and safe cryopreservation protocols are advisable. This study describes a protocol for aseptic vitrification of zygotes that results in high survival rates and minimizes the potential risk of contamination in liquid nitrogen during cooling and long-term storage. In mouse zygotes, there was no difference in efficiency as compared with a conventional open vitrification system. In IVF patients, aseptically vitrified zygotes showed no difference in blastocyst formation rate as compared with sibling zygotes kept in fresh culture. A clinical study comprising 173 cryo-cycles with a transfer of blastocysts originating from vitrified zygotes showed an ongoing pregnancy rate of 40.9%. The live birth rate per patient was 36.8%. A combination of good clinical results and increased safety conditions due to aseptic vitrification encourages the use of cryo-embryo transfer for patients with a suboptimal uterine environment in a fresh cycle.

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“…These contaminants may originate from extender, environment, processing equipments, liquid nitrogen, or skin surface of technicians (Bielanski 2012). The emergence of antibiotic-resistant strains of such opportunistic bacteria has made it difficult to control their spread despite the presence of antibiotics in bull semen extenders (Wierzbowski 1985).…”

Section: Resultsmentioning

confidence: 99%

Comparison of traditional antibiotic streptomycin with neomycin, polymyxin B, or colistin in extender for buffalo (Bubalus bubalis) bull spermatozoa

Qadeer¹,

Batool²,

Mehboob³

et al. 2013

Journal of Applied Animal Research

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This study was designed to compare the effect of traditional antibiotics streptomycinÁpenicillin (SP) with neomycinÁpenicillin (NP), polymyxinÁpenicillin (PP), and colistinÁpenicillin (CP) in extender against the postthaw quality and bacterial count of buffalo (Bubalus bubalis) bull spermatozoa. Qualifying semen ejaculates (n 018) from three Nili-Ravi buffalo bulls were splits into five aliquots and diluted (50 )10 (6 spermatozoa/ml; 378C) in extender containing SP, NP, PP, CP, or without antibiotics (control). The diluted semen was cooled to 48C in 2 hours and equilibrated for 4 hours. Cooled semen was filled in 0.5 ml French straws at 48C, kept over liquid nitrogen vapors (5 cm) for 10 minutes and then plunged in the liquid nitrogen for storage. For the assessment of post-thaw semen quality (motility, plasma membrane integrity (PMI), sperm viability) and total aerobic bacterial count (TABC), thawing was performed at 378C for 30 seconds. Sperm motility, PMI, sperm viability, and TABC remained similar (P!0.05) in all experimental extenders containing SP, NP, PP, CP, and extender without antibiotics. It is suggested that neomycin, polymyxin, or colistin in combination with penicillin did not deteriorate semen quality and may be suggested to replace streptomycin in traditional antibiotic combination for cryopreservation of Nilli-Ravi buffalo bull spermatozoa.

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A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease transmission in ET practices

Cited by 112 publications

References 85 publications

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Blastocyst transfer after aseptic vitrification of zygotes: an approach to overcome an impaired uterine environment

Comparison of traditional antibiotic streptomycin with neomycin, polymyxin B, or colistin in extender for buffalo (Bubalus bubalis) bull spermatozoa

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