Abstract:Excessive dependence on fossil fuels increases GHG emissions and carbon levels in the atmosphere, leading to climatic changes. This phenomenon can be reversed by capturing the carbon via “carbon capture and storage” (CCS) or “carbon capture and utilize” (CCU) technologies. In CCS methods, the captured carbon is stored in natural sinks (e.g., oceans), whereas, in CCU methods, the carbon is converted into useful products. Among CCU methods, the biological conversion of CO2 (BioConCO2) into value-added chemicals … Show more
“…Biological conversion of CO 2 , which aims to convert CO 2 into valuable biofuels, bioplastics, and chemicals through biological fixation, has garnered significant attention and is considered as a cutting-edge strategy in the fight against global warming . While photosynthesis by green plants is the primary CO 2 fixation mechanism in nature, it requires substantial land and water resources and is less efficient in solar energy utilization. , In contrast, natural photoautotrophic microorganisms (e.g., cyanobacteria, algae, lichens, and mosses) exhibit higher rates of CO 2 fixation.…”
3-Hydroxypropionic acid (3-HP) is a highly sought-after platform chemical serving as a precursor to a variety of high value-added chemical products. In this study, we designed and constructed a novel light-powered in vitro synthetic enzymatic biosystem comprising acetyl-CoA ligase, acetyl-CoA carboxylase, malonyl-CoA reductase, and phosphotransferase to efficiently produce 3-HP through CO 2 fixation from acetate, a costeffective and readily available substrate. The system employed natural thylakoid membranes (TMs) for the regeneration of adenosine triphosphate and nicotinamide adenine dinucleotide phosphate. Comprehensive investigations were conducted on the effects of buffer solutions, substrate concentrations, enzyme loading levels, and TMs loading levels to optimize the yield of 3-HP. Following optimization, a production of 0.46 mM 3-HP was achieved within 6 h from an initial 0.5 mM acetate, with a yield nearing 92%. This work underscores the simplicity of 3-HP production via an in vitro biomanufacturing platform and highlights the potential for incorporating TMs as a sustainable and environmentally friendly approach in biomanufacturing processes.
“…Biological conversion of CO 2 , which aims to convert CO 2 into valuable biofuels, bioplastics, and chemicals through biological fixation, has garnered significant attention and is considered as a cutting-edge strategy in the fight against global warming . While photosynthesis by green plants is the primary CO 2 fixation mechanism in nature, it requires substantial land and water resources and is less efficient in solar energy utilization. , In contrast, natural photoautotrophic microorganisms (e.g., cyanobacteria, algae, lichens, and mosses) exhibit higher rates of CO 2 fixation.…”
3-Hydroxypropionic acid (3-HP) is a highly sought-after platform chemical serving as a precursor to a variety of high value-added chemical products. In this study, we designed and constructed a novel light-powered in vitro synthetic enzymatic biosystem comprising acetyl-CoA ligase, acetyl-CoA carboxylase, malonyl-CoA reductase, and phosphotransferase to efficiently produce 3-HP through CO 2 fixation from acetate, a costeffective and readily available substrate. The system employed natural thylakoid membranes (TMs) for the regeneration of adenosine triphosphate and nicotinamide adenine dinucleotide phosphate. Comprehensive investigations were conducted on the effects of buffer solutions, substrate concentrations, enzyme loading levels, and TMs loading levels to optimize the yield of 3-HP. Following optimization, a production of 0.46 mM 3-HP was achieved within 6 h from an initial 0.5 mM acetate, with a yield nearing 92%. This work underscores the simplicity of 3-HP production via an in vitro biomanufacturing platform and highlights the potential for incorporating TMs as a sustainable and environmentally friendly approach in biomanufacturing processes.
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