A review of the methodological aspects of aspartic acid racemization analysis for use in forensic science

Waite, E. R.; Collins, Matthew J.; Ritz‐Timme, Stefanie; Schütz, H. W.; Cattaneo, Cristina; Borrman, H

doi:10.1016/s0379-0738(99)00081-x

Cited by 57 publications

(59 citation statements)

References 46 publications

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“…These methods have shown promise and can in the best of cases produce estimate values within ±5yrs of the actual age [13][14][15]17]. In teeth [27] it produced a 95% CI of ±8.7yrs across the ages and ±6.2yrs for ages less than 35.…”

Section: Discussionmentioning

confidence: 99%

“…This method is very laboratory and protocol-dependent [17], and achieves an average accuracy of ±5 years in bone tissue and ±3 years at best in perfectly preserved teeth. An age-dependent accumulation of D-aspartic acid has also been demonstrated in bone osteocalcin [16], which has led to age estimations within ±5 years (at a 95% prediction interval for the data).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determining ‘age at death’ for forensic purposes using human bone by a laboratory-based biomechanical analytical method

Zioupos

Williams

Christodoulou

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determining ‘age at death’ for forensic purposes using human bone by a laboratory-based biomechanical analytical method

Zioupos

Williams

Christodoulou

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

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“…Ten teeth were processed separately using a modified, simplified protocol. For these teeth, the crown was also separated from the root of the tooth, although this time the entire crown was washed in ddH 2 O and crushed using a liquid nitrogen-filled cryogenic impact grinder (SPEX 6850 Freezer Mill). A fine powder was obtained using a standard program with a run time of 15 min.…”

Section: Accelerator Ms Sample Preparationmentioning

confidence: 99%

Age Estimation in Forensic Sciences

Alkass

Buchholz

Ohtani

et al. 2010

Molecular & Cellular Proteomics

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Age determination of unknown human bodies is important in the setting of a crime investigation or a mass disaster because the age at death, birth date, and year of death as well as gender can guide investigators to the correct identity among a large number of possible matches. Traditional morphological methods used by anthropologists to determine age are often imprecise, whereas chemical analysis of tooth dentin, such as aspartic acid racemization, has shown reproducible and more precise results. In this study, we analyzed teeth from Swedish individuals using both aspartic acid racemization and radiocarbon methodologies. The rationale behind using radiocarbon analysis is that aboveground testing of nuclear weapons during the cold war (1955-1963) caused an extreme increase in global levels of carbon-14 ( 14 C), which has been carefully recorded over time. Forty-four teeth from 41 individuals were analyzed using aspartic acid racemization analysis of tooth crown dentin or radiocarbon analysis of enamel, and 10 of these were split and subjected to both radiocarbon and racemization analysis. Combined analysis showed that the two methods correlated well (R 2 ‫؍‬ 0.66, p < 0.05). Radiocarbon analysis showed an excellent precision with an overall absolute error of 1.0 ؎ 0.6 years. Aspartic acid racemization also showed a good precision with an overall absolute error of 5.4 ؎ 4.2 years. Whereas radiocarbon analysis gives an estimated year of birth, racemization analysis indicates the chronological age of the individual at the time of death. We show how these methods in combination can also assist in the estimation of date of death of an unidentified victim. This strategy can be of significant assistance in forensic casework involving dead victim identification. Molecular & Cellular Proteomics 9:1022-1030, 2010.

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“…Racemization of amino acids is one of alternative methods which is highly accurate for the age estimation (Waite et al 1999) and the teeth are the best organs for racemization based upon accuracy, simplicity, and time required. Nevertheless, depending on circumstances one can choose the organs such as bone, cartilage, and eye lens.…”

Section: History Of Age Estimation By Using Aspartic Acid Racemizationmentioning

confidence: 99%

Age Estimation by Using Aspartic Acid Racemization from Purified Elastin of Aorta

Komutrattananont¹,

Mahakkanukrauh²

2017

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ABSTRAK ABSTRACTAge estimation is the one of biological profiles which plays an important role in identification in forensic field. The application of biochemical approach known 171 Age Estimation by Aspartic Acid Racemization Med & Health Dec 2017;12(2): 170-178 depend on the variation methods and skill of the specialist (Dobberstein et al. 2010). However, there are still high accuracy methods in age estimation which complicate the procedure more. One of these methods which has been accomplished in age estimation is amino acid racemization. AMINO ACID RACEMIZATIONAmino acids are vital components in living organisms and are subunit structure of all proteins. The amino acid structure is composed of a carbon atom (C) which has four different groups include an amino group (-NH 2 ), a carboxyl group (-COOH), a hydrogen atom (-H), and side chain or Rgroup (-R). Side chain is different part of amino acids that depends upon the kind of as aspartic acid racemization in human provides more accuracy and less error of estimated age in age determination. During aging, amino acids within protein transform between D-and L-form (racemization), which results in increasing concentration of D-amino acids. This change of amino acids is related to aging. Aspartic acid has been generally applied in racemization because it has fastest rate of racemization. Racemization of aspartic acid not only takes place in teeth and many kinds of bone, but also can occur in the flexible structure which contain mostly elastin. For example, skin yellow ligament, lung parenchyma, and aorta. Elastin is essential protein which is comprised most composition of several structures and it is major content in the elastic fibers. Additionally, elastin is the longest lasting protein in the body with slow metabolic turnover rate. Recently, there is a purification technique to obtain only single protein in human aorta.There was a good correlation between degree of racemization (D/L ratio) and age. Therefore, aspartic acid racemization by purifies elastin from aorta is a one of indicator for age estimation. This review provides an overview of aspartic acid racemization, elastin protein, purification method bases on racemization and the application of D/L ratio by purified of elastin in artery for age estimation.

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A review of the methodological aspects of aspartic acid racemization analysis for use in forensic science

Cited by 57 publications

References 46 publications

Determining ‘age at death’ for forensic purposes using human bone by a laboratory-based biomechanical analytical method

Determining ‘age at death’ for forensic purposes using human bone by a laboratory-based biomechanical analytical method

Age Estimation in Forensic Sciences

Age Estimation by Using Aspartic Acid Racemization from Purified Elastin of Aorta

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