A review of the environmental safety of the CP4 EPSPS protein

Lean, Morven Mc

doi:10.1051/ebr:2012001

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…In recent years, GM crop cultivation has developed rapidly and led to increased crop yield, reduced labor costs, decreased pesticide use, and so on. At the same time, the unintended food and environmental safety issues caused by GM crops have also been widely studied [6,[28][29][30][31]. Detection of exogenous ingredients in GM crops, their products, and even their growing environments is becoming more and more urgent and important.…”

Section: Discussionmentioning

confidence: 99%

Establishment of an ELISA Method for Quantitative Detection of PAT/pat in GM Crops

et al. 2022

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The phosphinothricin N-acetyltransferase gene (pat) is widely used to confer resistance to the herbicide phosphinothricin for genetically modified (GM) crops. A quantitative sandwich enzyme-linked immunosorbent assay (ELISA) is developed to detect PAT/pat in GM crops. Two anti-PAT/pat monoclonal antibodies (mAbs), 1F5-1F2 and 1B6-2D3, with titers of 1: 1,024,000 and 1: 896,000, respectively, against overexpressed His-PAT/pat, were screened out, raised, and characterized. An ELISA method was established with the 1F5-2F2 mAb for capture and the biotin-labeled 1B6-2D3 mAb for detection of PAT/pat. The linear detection range of the method was approximately 1.5625–12.5 ng/mL, with a sensitivity of 0.085 ng/mL and a coefficient of variation (CV) less than 5.0%. No cross-reactivity was found with other herbicide resistance proteins, especially PAT/bar. The established sensitive and specific ELISA was successfully applied in the detection of PAT/pat expression in GM crops.

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Section: Discussionmentioning

confidence: 99%

Establishment of an ELISA Method for Quantitative Detection of PAT/pat in GM Crops

et al. 2022

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Effects of temperature, water content, pH and soil sterilization on the degradation of CP4-EPSPS protein released from herbicide-tolerant corn leaves in the soil

Zeng

Wang

Feng

et al. 2018

Soil Biology and Biochemistry

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Characterization of the ecological interactions of Roundup Ready 2 Yield® soybean, MON 89788, for use in ecological risk assessment

et al. 2015

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As part of an ecological risk assessment, Roundup Ready 2 Yield® soybean (MON 89788) was compared to a conventional control soybean variety, A3244, for disease and arthropod damage, plant response to abiotic stress and cold, effects on succeeding plant growth (allelopathic effects), plant response to a bacterial symbiont, and effects on the ability of seed to survive and volunteer in a subsequent growing season. Statistically significant differences between MON 89788 and A3244 were considered in the context of the genetic variation known to occur in soybean and were assessed for their potential impact on plant pest (weed) potential and adverse environmental impact. The results of these studies revealed no effects of the genetic modification that would result in increased pest potential or adverse environmental impact of MON 89788 compared with A3244. This paper illustrates how such characterization studies conducted in a range of environments where the crop is grown are used in an ecological risk assessment of the genetically modified (GM) crop. Furthermore, risk assessors and decision makers use this information when deciding whether to approve a GM crop for cultivation in-or grain import into-their country.

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A review of the environmental safety of the CP4 EPSPS protein

Cited by 7 publications

References 22 publications

Establishment of an ELISA Method for Quantitative Detection of PAT/pat in GM Crops

Establishment of an ELISA Method for Quantitative Detection of PAT/pat in GM Crops

Effects of temperature, water content, pH and soil sterilization on the degradation of CP4-EPSPS protein released from herbicide-tolerant corn leaves in the soil

Characterization of the ecological interactions of Roundup Ready 2 Yield® soybean, MON 89788, for use in ecological risk assessment

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