A Review of the Effects of the Biopesticides Bacillus thuringiensis Serotypes israelensis (Bti) and kurstaki (Btk) in Amphibians

Empey, Madelaine; Lefebvre-Raine, Molly; Gutierrez-Villagómez, Juan Manuel; Langlois, Valérie S.; Trudeau, Vance L.

doi:10.1007/s00244-021-00842-2

Cited by 8 publications

(6 citation statements)

References 43 publications

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“…[In part, misidentification.] Viña-Albornoz et al (2020: 277); Antoniazzi et al (2021: 47); Attademo et al (2021: their Table 1); Catalano et al (2021: 635, their Tables 1 and 2); Curi et al (2021: 2, 3); de Oliveira et al (2021: 16); Demartín et al (2021: their Table 2); Empey et al (2021: 796, their Table 1); Ferraro et al (2021: 396, their Fig. 5, their supplementary data S1–S7); González et al (2021: 4, 15, their Figs.…”

Section: Species Accountsmentioning

confidence: 99%

The Taxonomic Status of Physalaemus cuqui Lobo, 1993, with the Description of a New Species of Physalaemus (Anura: Leptodactylidae) from Argentina and Paraguay

Cardozo,

Tomatis,

Duport Bru

et al. 2023

Herpetological Monographs

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Section: Species Accountsmentioning

confidence: 99%

The Taxonomic Status of Physalaemus cuqui Lobo, 1993, with the Description of a New Species of Physalaemus (Anura: Leptodactylidae) from Argentina and Paraguay

Cardozo,

Tomatis,

Duport Bru

et al. 2023

Herpetological Monographs

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“…aegypti [44]. Both densoviruses and Bti are pathogenic to mosquitoes and have no toxic effect on unrelated species [51][52][53]. Studies on recombinant densoviruses with improved insecticidal efficacy are much needed, as limited literature is present.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus

Batool

Álam

Liu

et al. 2022

Toxins

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Mosquito densoviruses (MDVs) are mosquito-specific viruses that are recommended as mosquito bio-control agents. The MDV Aedes aegypti densovirus (AeDNV) is a good candidate for controlling mosquitoes. However, the slow activity restricts their widespread use for vector control. In this study, we introduced the Bacillus thuringiensis (Bti) toxin Cry11Aa domain II loop α8 and Cyt1Aa loop β6-αE peptides into the AeDNV genome to improve its mosquitocidal efficiency; protein expression was confirmed using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). Recombinant plasmids were transfected into mosquito C6/36 cell lines, and the expression of specific peptides was detected through RT-PCR. A toxicity bioassay against the first instar Aedes albopictus larvae revealed that the pathogenic activity of recombinant AeDNV was significantly higher and faster than the wild-type (wt) viruses, and mortality increased in a dose-dependent manner. The recombinant viruses were genetically stable and displayed growth phenotype and virus proliferation ability, similar to wild-type AeDNV. Our novel results offer further insights by combining two mosquitocidal pathogens to improve viral toxicity for mosquito control.

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“…israelensis (Bti) is a Gram-positive bacterium first isolated from soil samples of a mosquito breeding site in Israel . Currently, Bti-based products are used in urban as well as rural settings to prevent and control the dissemination of diseases by mosquitos, decrease the nuisance of biting insects, and increase human population comfort. − …”

Section: Introductionmentioning

confidence: 99%

Frogs Respond to Commercial Formulations of the Biopesticide Bacillus thuringiensis var. israelensis, Especially Their Intestine Microbiota

Gutierrez-Villagómez

Patey

et al. 2021

Environ. Sci. Technol.

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It is generally believed that Bacillus thuringiensis var. israelensis (Bti) biopesticides are harmless to non-target organisms; however, new research shows controversial results. We exposed acutely and chronicallyLithobates sylvaticusandAnaxyrus americanus tadpoles until metamorphic climax to VectoBac 200G (granules) and VectoBac 1200L (aqueous suspension) at 300–20,000 ITU/L covering field-relevant concentrations and higher. The data show that the exposure parameters tested did not affect significantly the survival, total length, total weight, hepatosomatic index, gonadosomatic index, the expression of genes of interest (i.e., related to xenobiotic exposure, oxidative stress, and metamorphosis), and the intestine tissue layer detachment ofL. sylvaticusandA. americanus in a concentration–response pattern. In contrast, VectoBac 200G significantly increased the median time to metamorphosis ofL. sylvaticus tadpoles by up to 3.5 days and decreased the median by up to 1 day inA. americanus. VectoBac 1200L significantly increased the median time to metamorphosis ofL. sylvaticusandA. americanustadpoles by up to 4.5 days. Also, the exposure to VectoBac 200G and 1200L altered the intestine bacterial community composition inA. americanus at application rates recommended by the manufacturer, which led to an increase in the relative abundance of Verrucomicrobia, Firmicutes, Bacteroidetes, and Actinobacteria. Changes in the intestine microbiota might impact the fitness of individuals, including the susceptibility to parasitic infections. Our results indicate that the effect of Bti commercial products is limited; however, we recommend that Bti-spraying activities in amphibian-rich ecosystems should be kept minimal until there is more conclusive research to assess if the changes in the time to metamorphosis and microbiota can lead to negative outcomes in amphibian populations and, eventually, the functioning of ecosystems.

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A Review of the Effects of the Biopesticides Bacillus thuringiensis Serotypes israelensis (Bti) and kurstaki (Btk) in Amphibians

Cited by 8 publications

References 43 publications

The Taxonomic Status of Physalaemus cuqui Lobo, 1993, with the Description of a New Species of Physalaemus (Anura: Leptodactylidae) from Argentina and Paraguay

The Taxonomic Status of Physalaemus cuqui Lobo, 1993, with the Description of a New Species of Physalaemus (Anura: Leptodactylidae) from Argentina and Paraguay

Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus

Frogs Respond to Commercial Formulations of the Biopesticide Bacillus thuringiensis var. israelensis, Especially Their Intestine Microbiota

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