A review of sorting, separation and isolation of cells and microbeads for biomedical applications: microfluidic approaches

Dalili, Arash; Samiei, Ehsan; Hoorfar, Mina

doi:10.1039/c8an01061g

Cited by 214 publications

(145 citation statements)

References 318 publications

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“…As a robust biomedical device, the miniaturized microfluidic platform has been utilized in various fields such as tissue engineering, microparticles' fabrication, drug delivery, point‐of‐care testing (POCT), and tumor screening . With the huge advancement of microfabrication methods, microfluidic platforms are increasingly employed to study basic and applied research in oncology due to the remarkable advantages of low cost, well‐controlled microstructures, reduced sample consumptions, rapid fluid processing, and satisfactory detection sensitivity . In this review, we highlight the recent key developments of microfluidic platforms applied in the aforementioned two topics of cancer metastasis: CTCs' isolation and tumor models recapitulating the metastatic microenvironment (tumor‐on‐a‐chip) ( Scheme ).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Microfluidic Platforms Applied in Cancer Metastasis: Circulating Tumor Cells' (CTCs) Isolation and Tumor‐On‐A‐Chip

Lin

Luo

et al. 2019

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Cancer remains the leading cause of death worldwide despite the enormous efforts that are made in the development of cancer biology and anticancer therapeutic treatment. Furthermore, recent studies in oncology have focused on the complex cancer metastatic process as metastatic disease contributes to more than 90% of tumor‐related death. In the metastatic process, isolation and analysis of circulating tumor cells (CTCs) play a vital role in diagnosis and prognosis of cancer patients at an early stage. To obtain relevant information on cancer metastasis and progression from CTCs, reliable approaches are required for CTC detection and isolation. Additionally, experimental platforms mimicking the tumor microenvironment in vitro give a better understanding of the metastatic microenvironment and antimetastatic drugs' screening. With the advancement of microfabrication and rapid prototyping, microfluidic techniques are now increasingly being exploited to study cancer metastasis as they allow precise control of fluids in small volume and rapid sample processing at relatively low cost and with high sensitivity. Recent advancements in microfluidic platforms utilized in various methods for CTCs' isolation and tumor models recapitulating the metastatic microenvironment (tumor‐on‐a‐chip) are comprehensively reviewed. Future perspectives on microfluidics for cancer metastasis are proposed.

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Section: Introductionmentioning

confidence: 99%

Recent Advances in Microfluidic Platforms Applied in Cancer Metastasis: Circulating Tumor Cells' (CTCs) Isolation and Tumor‐On‐A‐Chip

Lin

Luo

et al. 2019

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“…[1–5]. Microfluidic systems have been recognized as a practical tool for efficient sorting with the benefit of microfabrication, and several types of microfluidic cell sorters are commercially available [6–10]. Instead of employing external force fields in the active sorting [11, 12], the passive sorting relies on the inherent microfluidic features underlying the flow field, channel geometry, and particle interactions.…”

Section: Introductionmentioning

confidence: 99%

Two‐phase flow in microfluidic‐chip design of hydrodynamic filtration for cell particle sorting

2020

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As one of the flow-based passive sorting, the hydrodynamic filtration using a microfluidicchip has shown to effectively separate into different sizes of subpopulations from cell or particle suspensions. Its model framework involving two-phase Newtonian or generalized Newtonian fluid (GNF) was developed, by performing the complete analysis of laminar flow and complicated networks of main and multiple branch channels. To predict rigorously what occurs in flow fields, we estimated pressure drop, velocity profile, and the ratio of the flow fraction at each branch point, in which the analytical model was validated with numerical flow simulations. As a model fluid of the GNF, polysaccharide solution based on Carreau type was examined. The objective parameters aiming practical channel design include the number of the branches and the length of narrow section of each branch for arbitrary conditions. The flow fraction and the number of branches are distinctly affected by the viscosity ratio between feed and side flows. As the side flow becomes more viscous, the flow fraction increases but the number of branches decreases, which enables a compact chip designed with fewer branches being operated under the same throughput. Hence, our rational design analysis indicates the significance of constitutive properties of each stream.

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“…Microfluidic separation techniques have been reviewed recently [1][2][3][4][5][6] with some papers focusing on inertial devices in particular [7][8][9][10][11] . Inertial separation is solely based on channel geometry and hydrodynamic forces 12 without requiring cell manipulation by external forces which makes it a robust and easy-to-use method.…”

Section: Introductionmentioning

confidence: 99%

Spiral microfluidic devices for cell separation and sorting in bioprocesses

2019

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Inertial microfluidic systems have been arousing interest in medical applications due to their simple and cost-efficient use. However, comparably small sample volumes in the microliter and milliliter ranges have so far prevented efficient applications in continuous bioprocesses. Nevertheless, recent studies suggest that these systems are well suited for cell separation in bioprocesses because of their facile adaptability to various reactor sizes and cell types. This review will discuss potential applications of inertial microfluidic cell separation systems in downstream bioprocesses and depict recent advances in inertial microfluidics for bioprocess intensification. This review thereby focusses on spiral microchannels that separate particles at a moderate Reynolds number in a laminar flow (Re < 2300) according to their size by applying lateral hydrodynamic forces. Spiral microchannels have already been shown to be capable of replacing microfilters, extracting dead cells and debris in perfusion processes, and removing contaminant microalgae species. Recent advances in parallelization made it possible to process media on a liter-scale, which might pave the way toward industrial applications.

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A review of sorting, separation and isolation of cells and microbeads for biomedical applications: microfluidic approaches

Abstract: We have reviewed the microfluidic approaches for cell/particle isolation and sorting, and extensively explained the mechanism behind each method.

Cited by 214 publications

References 318 publications

Recent Advances in Microfluidic Platforms Applied in Cancer Metastasis: Circulating Tumor Cells' (CTCs) Isolation and Tumor‐On‐A‐Chip

Recent Advances in Microfluidic Platforms Applied in Cancer Metastasis: Circulating Tumor Cells' (CTCs) Isolation and Tumor‐On‐A‐Chip

Two‐phase flow in microfluidic‐chip design of hydrodynamic filtration for cell particle sorting

Spiral microfluidic devices for cell separation and sorting in bioprocesses

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