A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

Matcher, Stephen J.

doi:10.1063/1.3116620

Cited by 33 publications

(30 citation statements)

References 50 publications

(48 reference statements)

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“…Rotating the polarization of the incident laser beam allowed us to measure the polarimetric response of collagen fibrils in different regions of the tissue. As previously demonstrated [33][34][35], the SHG signals from collagen fibrils are higher when the polarization is parallel to the local direction of the fibrils, revealing the general alignment of the fibrils in the region of interest. Therefore, the general fibrils' orientation over the field of view can be observed on the corresponding polarimetric plot (Figure 2c) indicating the SHG intensity detected as a function of the incident polarization.…”

Section: Comparison Between Forward and Backward Shg Imagessupporting

confidence: 60%

Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage

Houle

Couture

Bancelin

et al. 2015

Journal of Biophotonics

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Collagen ultrastructure plays a central role in the function of a wide range of connective tissues. Studying collagen structure at the microscopic scale is therefore of considerable interest to understand the mechanisms of tissue pathologies. Here, we use second harmonic generation microscopy to characterize collagen structure within bone and articular cartilage in human knees. We analyze the intensity dependence on polarization and discuss the differences between Forward and Backward images in both tissues. Focusing on articular cartilage, we observe an increase in Forward/Backward ratio from the cartilage surface to the bone. Coupling these results to numerical simulations reveals the evolution of collagen fibril diameter and spatial organization as a function of depth within cartilage.

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Section: Comparison Between Forward and Backward Shg Imagessupporting

confidence: 60%

Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage

Houle

Couture

Bancelin

et al. 2015

Journal of Biophotonics

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“…The collagen molecule is excited by two near-infrared incident photons, which come together to produce a visible photon with exactly half the wavelength and twice the energy. This photon can be detected at half the wavelength of that used to excite the sample and the intensity of the resulting SHG image is influenced by the quantity of collagen present as well as the fibril organisation (Bayan et al, 2009;Matcher, 2009).…”

Section: Rm Delaine-smithmentioning

confidence: 99%

“…Mesenchymal progenitor cells play a vital role in bone regenerative medicine and tissue engineering strategies, and to be clinically useful they should be readily available with the potential to undergo osteogenesis. Mesenchymal stem or stromal cells (MSCs) harvested from bone marrow have shown great potential as an autologous bone cell source with self-renewing and multipotent properties capable of in vitro differentiation along the osteogenic lineage (Jaiswal et al, 1997;Mauney et al, 2004;Pittenger et al, 1999). However, bone marrow extraction carries the risk of donor site morbidity and only a small number of MSCs are obtained from bone marrow, which are difficult to expand to sufficient numbers in vitro.…”

Section: Introductionmentioning

confidence: 99%

Matrix production and collagen structure are enhanced in two types of osteogenic progenitor cells by a simple fluid shear stress stimulus

MacNeil²,

Reilly³

2012

eCM

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Mesenchymal progenitor cells play a vital role in bone regenerative medicine and tissue engineering strategies.To be clinically useful osteoprogenitors should be readily available with the potential to form bone matrix. While mesenchymal stromal cells from bone marrow have shown promise for tissue engineering, they are obtained in small numbers and there is risk of donor site morbidity. Osteogenic progenitor cells derived from dermal tissue may provide a more abundant and easily expandable source of cells. Bone turnover in vivo is regulated by mechanical forces, particularly oscillatory fluid shear stresses (FSS), and in vitro osteogenic progenitors have been shown to be regulated by mechanical stimuli. The aim of this study was to assess what effect osteogenic media and FSS, generated by a simple rocking platform, had on cell behaviour and matrix production in human progenitor dermal fibroblasts (HDFs) and the embryonic stem cell-derived mesenchymal progenitor cell line (hES-MP).Osteogenic media stimulated alkaline phosphatase activity (ALP) and calcium deposition in HDFs. The addition of FSS further enhanced ALP activity and mineralised matrix deposition in both progenitor cells cultured in osteogenic media. Both types of progenitor cell subjected to FSS showed increases in collagen secretion and apparent collagen organisation as imaged by second harmonic generation.

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“…OCT in combination with arthroscopy has been shown to be capable to detect structural changes in the cartilage during surgery . Also non‐linear microscopic methods have been developed to analyze structural changes in the articular cartilage . Nevertheless, deep cartilage layers, cartilage‐bone boundary and subchondral bone are usually inaccessible with conventional OCT or microscopy‐based approaches, whereas the structural depth‐resolved imaging of cartilage‐bone interface is of great importance for improving the currently used arthroscopic methods.…”

Section: Introductionmentioning

confidence: 99%

Imaging of subchondral bone by optical coherence tomography upon optical clearing of articular cartilage

et al. 2015

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Optical clearing is an effective method to reduce light scattering of biological tissues that provides significant enhancement of light penetration into the biological tissues making non-invasive diagnosis more feasible. In current report Optical Coherence Tomography (OCT) in conjunction with optical clearing is applied for assessment of deep cartilage layers and cartilage-bone interface. The solution of Iohexol in water has been used as an optical clearing agent. The cartilage-bone boundary becomes visible after 15 min of optical clearing that enabling non-invasive estimation of its roughness: Sa = 10 ± 1 µm. The results show that for 0.9 mm thick cartilage optical clearing is stopped after 50 min with an increase of refractive index from 1.386 ± 0.008 to 1.510 ± 0.009. Current approach enables more reliable detection of arthroscopically inaccessible regions, including cartilage-bone boundary and subchondral bone, and potentially improves accuracy of the osteoarthritis diagnosis.

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A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

Cited by 33 publications

References 50 publications

Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage

Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage

Matrix production and collagen structure are enhanced in two types of osteogenic progenitor cells by a simple fluid shear stress stimulus

Imaging of subchondral bone by optical coherence tomography upon optical clearing of articular cartilage

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