A review of protocols for engineering human cardiac organoids

Wu, Fujian; He, Qian; Li, Furong; Yang, Xiaofei

doi:10.1016/j.heliyon.2023.e19938

Cited by 2 publications

(1 citation statement)

References 62 publications

(73 reference statements)

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“…Since 2021, there have been multiple studies describing the generation of cardiac organoids to validate the mimicry of cardiac structures such as chambers, epicardium/myocardium, and atrium/ventricle-reminiscent regions (see reviews for more details [ 18 , 19 ]). In this section, we will highlight the recent major advances and perspectives in this field.…”

Section: D Heart Modelsmentioning

confidence: 99%

The new era of cardiovascular research: revolutionizing cardiovascular research with 3D models in a dish

Yang,

Kiskin

et al. 2024

Medical Review

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Cardiovascular research has heavily relied on studies using patient samples and animal models. However, patient studies often miss the data from the crucial early stage of cardiovascular diseases, as obtaining primary tissues at this stage is impracticable. Transgenic animal models can offer some insights into disease mechanisms, although they usually do not fully recapitulate the phenotype of cardiovascular diseases and their progression. In recent years, a promising breakthrough has emerged in the form of in vitro three-dimensional (3D) cardiovascular models utilizing human pluripotent stem cells. These innovative models recreate the intricate 3D structure of the human heart and vessels within a controlled environment. This advancement is pivotal as it addresses the existing gaps in cardiovascular research, allowing scientists to study different stages of cardiovascular diseases and specific drug responses using human-origin models. In this review, we first outline various approaches employed to generate these models. We then comprehensively discuss their applications in studying cardiovascular diseases by providing insights into molecular and cellular changes associated with cardiovascular conditions. Moreover, we highlight the potential of these 3D models serving as a platform for drug testing to assess drug efficacy and safety. Despite their immense potential, challenges persist, particularly in maintaining the complex structure of 3D heart and vessel models and ensuring their function is comparable to real organs. However, overcoming these challenges could revolutionize cardiovascular research. It has the potential to offer comprehensive mechanistic insights into human-specific disease processes, ultimately expediting the development of personalized therapies.

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Section: D Heart Modelsmentioning

confidence: 99%

The new era of cardiovascular research: revolutionizing cardiovascular research with 3D models in a dish

Yang,

Kiskin

et al. 2024

Medical Review

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Advances in cardiac organoid technology: current trends and standardized quality assessment for toxicity testing in preclinical models

Lee,

Lee

2024

Organoid

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Cardiotoxicity is a prominent cause of drug withdrawal, impacting drug development during preclinical and clinical phases, as well as post-market approval. Traditional 2-dimensional cell culture models, despite being resource-rich, suffer from a suboptimal physiological environment. Animal models provide an in vivo setting; however, their use is declining due to interspecies variability and ethical concerns. Cardiac organoids, which are derived from human induced pluripotent stem cells, incorporate human genetic information and contain various cell types within a 3-dimensional environment that mimics the human heart. As a result, they are becoming increasingly popular in the development of preclinical models for drug testing. To effectively use cardiac organoids as preclinical models, a standardized quality assessment is crucial. This review discusses current trends in cardiac organoid generation and examines recent analytical techniques for quality assessment.

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A review of protocols for engineering human cardiac organoids

Cited by 2 publications

References 62 publications

The new era of cardiovascular research: revolutionizing cardiovascular research with 3D models in a dish

The new era of cardiovascular research: revolutionizing cardiovascular research with 3D models in a dish

Advances in cardiac organoid technology: current trends and standardized quality assessment for toxicity testing in preclinical models

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