A Review of Preanalytical Factors Affecting Molecular, Protein, and Morphological Analysis of Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue: How Well Do You Know Your FFPE Specimen?

Bass, B. Paige; Engel, Kelly B.; Greytak, Sarah R.; Moore, Helen M.

doi:10.5858/arpa.2013-0691-ra

Cited by 243 publications

(210 citation statements)

References 140 publications

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…Soft tissues can be separated from sclerotic tissues and placed in different blocks. In most bone materials with metastatic lesion recognition, the tumor part usually does not require decalcification because most tumors damage the bone structure (103,104).…”

Section: Special Material: Bonementioning

confidence: 99%

“…In a material that has been decalcified in an experimental study conducted in Colorado University, it was reported that as basophilia increases in the bone trabeculae, the reliability of molecular treatment also increases. This is related to the eosinophilic appearance of the fully decalcified bone trabecula while basophilia reflects less extensive decalcification (103,104).…”

Section: Special Material: Bonementioning

confidence: 99%

See 1 more Smart Citation

Techniques for maximizing the performance of molecular pathology testing: responsibilities of all pathologists

Uzun

Sarıoğlu²

2017

TJPATH

View full text Add to dashboard Cite

Molecular pathological analysis has an expanding role in patient diagnosis and management. The performance of these techniques relies on excellent laboratory procedures. However, the crucial step is obtaining the best samples for molecular analysis. Archiving and selection of these are the responsibilities of all pathologists even if they are not working at a center with molecular pathological facilities.This review focuses on the features of different types of materials for molecular pathological analysis. Many steps that might affect the results, including communication between the pathologist and the oncology team, features of different types of materials (cytological, tissue blocks, biopsy, circulating tumor cells (CTCs) and cell-free circulating nucleic acids), effects of tissue processing, methods for selecting the best material, and tissue saving and tumor enrichment methods are discussed. The procedures for referral to a center for molecular pathological analysis are also mentioned.Awareness of the importance of the cytopathological and histopathological material of the patients for future molecular pathological analysis by pathologists is of the utmost importance.Key Words: Molecular Pathology, Tissue saving, Tumor enrichment, Tumor percentage INTRODUCTION "Molecular Pathology" is one of the pathological methods with expanding role in patient diagnosis, prediction of prognosis and treatment. Tissue and cellular material from patients have always been valued in pathology laboratories; however they gain additional importance in the molecular pathology era with the increase in new disease classifications according to molecular changes and new targeted therapy options being determined with predictive molecular testing.While the value of diseased and normal tissue is expanding with the advances in immunohistochemistry and molecular pathology, minimally invasive techniques have taken the place of radical processes in tissue sampling along with developments in the technology. These techniques are better for the comfort of the patient but they also have disadvantages, as they provide smaller amounts of tissue for diagnosis. The management of tissues obtained with these techniques can be problematic for pathologists. The application of individualized treatment regimens developed in recent years has depended on molecular studies. The adequacy of the obtained material and its reliability for molecular investigation are of utmost importance in many cases.The diagnosis of lung carcinomas is usually made with small biopsies or cytological materials and these materials are generally the only options for further studies. Transthoracic, transbronchial fine needle aspirations, forceps biopsies, bronchial brushes, and washes are some of these materials (1). A molecular examination requires the best process and good sample quality. The most important factor for the success of molecular tests is the number of tumor cells and the percentage of mutant cells (2). The requirements for tumor cell number and mutation perc...

show abstract

Section: Special Material: Bonementioning

confidence: 99%

Section: Special Material: Bonementioning

confidence: 99%

Techniques for maximizing the performance of molecular pathology testing: responsibilities of all pathologists

Uzun

Sarıoğlu²

2017

TJPATH

View full text Add to dashboard Cite

show abstract

“…Formalin fixation and paraffin embedding was performed as previously described (12). Five micron thick sections were cut from each paraffin block and stained with haematoxylin and eosin (H and E) staining.…”

Section: Histopathologymentioning

confidence: 99%

The correlation of histopathological subcutaneous adipose tissue changes with post bariatric wound healing complications

Sakka¹,

Elaskandrany

2016

Int Surg J

View full text Add to dashboard Cite

“…1-3). [1][2][3] Effects attributable to pre-analytical factors can be severe, resulting in misdiagnosis 4 and false discovery of biomarkers of disease, 5,6 and also both extensive and elusive, as 48%-58% of biobanked tissue collected and stored using institution-approved SOPs was deemed unfit for RNA analysis. 7,8 Thus, the availability of human biospecimens of sufficiently high quality continues to be a pressing need of the medical research community.…”

mentioning

confidence: 99%

“…However, in most instances, SOPs are institution-specific, which can confound analysis of biospecimens collected at different hospitals or those obtained from different biobanks or biorepositories. Use of institution-specific SOPs may also preclude external validation of research findings, as well as meta-analysis efforts employed by both regulatory organizations, such as the United States Food and Drug Administration (USFDA) and the European Medicines Agency (EMA), and scientific organizations that aim to identify pre-analytical factors and their effects [1][2][3] or potential markers of biospecimen quality. 9 While there is a real and present need for a more global method of standardization and validation, universal adoption of a single collection of SOPs is impractical due to the different logistical and financial constraints associated with individual facilities.…”

mentioning

confidence: 99%

National Cancer Institute Biospecimen Evidence-Based Practices: A Novel Approach to Pre-analytical Standardization

Engel¹,

Vaught

Moore

2014

Biopreservation and Biobanking

Self Cite

View full text Add to dashboard Cite

Variable biospecimen collection, processing, and storage practices may introduce variability in biospecimen quality and analytical results. This risk can be minimized within a facility through the use of standardized procedures; however, analysis of biospecimens from different facilities may be confounded by differences in procedures and inferred biospecimen quality. Thus, a global approach to standardization of biospecimen handling procedures and their validation is needed. Here we present the first in a series of procedural guidelines that were developed and annotated with published findings in the field of human biospecimen science. The series of documents will be known as NCI Biospecimen Evidence-Based Practices, or BEBPs. Pertinent literature was identified via the National Cancer Institute (NCI) Biospecimen Research Database (brd.nci .nih.gov) and findings were organized by specific biospecimen pre-analytical factors and analytes of interest (DNA, RNA, protein, morphology). Meta-analysis results were presented as annotated summaries, which highlight concordant and discordant findings and the threshold and magnitude of effects when applicable. The detailed and adaptable format of the document is intended to support the development and execution of evidence-based standard operating procedures (SOPs) for human biospecimen collection, processing, and storage operations.

show abstract

A Review of Preanalytical Factors Affecting Molecular, Protein, and Morphological Analysis of Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue: How Well Do You Know Your FFPE Specimen?

Cited by 243 publications

References 140 publications

Techniques for maximizing the performance of molecular pathology testing: responsibilities of all pathologists

Techniques for maximizing the performance of molecular pathology testing: responsibilities of all pathologists

The correlation of histopathological subcutaneous adipose tissue changes with post bariatric wound healing complications

National Cancer Institute Biospecimen Evidence-Based Practices: A Novel Approach to Pre-analytical Standardization

Contact Info

Product

Resources

About