A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective

Yao, Haoyingye; Wang, Junqiao; Yin, Jingdong; Nie, Shaoping; Xie, Mingyong

doi:10.1016/j.foodres.2021.110290

Cited by 202 publications

(86 citation statements)

References 176 publications

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“…The signals at 5.10/109.31 ppm and 4.23/83.79 ppm indicated the presence of H-1/C-1 and H-4/C-4 of 1,5-α-L-Araf, whereas the C-2 and C-5 of 1,5-α-L-Araf were measured to be 81.20 and 67.74 ppm [25,27]. Besides, the signal at 5.17 ppm was attributed to the H-1 of T-α-L-Araf [25,28]. The peaks at 5.04/99.52 ppm were attributed to H-1/C-1 of 1,4-α-D-GalAp, whereas H-3, C-2, and C-5 of 1,4-α-D-GalAp were also determined to be 4.13, 69.81, and 73.28 ppm [20,25].…”

Section: Ft-ir and Nmr Spectra Of Llpsmentioning

confidence: 98%

“…The signals at 5.26, 4.02, and 1.26 ppm were assigned to H-1, H-2, and H-6 of 1,2-α-L-Rhap [20,25,26]. The signals at 5.10/109.31 ppm and 4.23/83.79 ppm indicated the presence of H-1/C-1 and H-4/C-4 of 1,5-α-L-Araf, whereas the C-2 and C-5 of 1,5-α-L-Araf were measured to be 81.20 and 67.74 ppm [25,27]. Besides, the signal at 5.17 ppm was attributed to the H-1 of T-α-L-Araf [25,28].…”

Section: Ft-ir and Nmr Spectra Of Llpsmentioning

confidence: 99%

“…Besides, the signal at 5.17 ppm was attributed to the H-1 of T-α-L-Araf [25,28]. The peaks at 5.04/99.52 ppm were attributed to H-1/C-1 of 1,4-α-D-GalAp, whereas H-3, C-2, and C-5 of 1,4-α-D-GalAp were also determined to be 4.13, 69.81, and 73.28 ppm [20,25]. In addition, peaks at 4.98/100.47 were attributed to H-1/C-1 of 1,4-α-D-GalAMep, whereas the H/C signals of the methyl signal of 1,4-α-D-GalAMep were measured at 3.82 and 52.80 ppm [20,25].…”

Section: Ft-ir and Nmr Spectra Of Llpsmentioning

confidence: 99%

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Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Feng

Huang

et al. 2021

Foods

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Lotus leaves are often discarded as byproducts in the lotus industry. Polysaccharides are regarded as one of the essentially bioactive components in lotus leaves. Therefore, in order to promote the application of lotus leaves in the functional food industry, the deep eutectic solvent (DES) assisted extraction of polysaccharides from lotus leaves (LLPs) was optimized, and structural and biological properties of LLPs extracted by DES and hot water were further investigated. At the optimal extraction conditions (water content of 61.0% in DES, extraction temperature of 92 °C, liquid-solid ratio of 31.0 mL/g and extraction time of 126 min), the maximum extraction yield (5.38%) was obtained. Furthermore, LLP-D extracted by DES and LLP-W extracted by hot water possessed the same sugar residues, such as 1,4-α-D-GalAp, 1,4-α-D-GalAMep, 1,3,6-β-D-Galp, 1,4-β-D-Galp, 1,5-α-L-Araf, and 1,2-α-L-Rhap, suggesting the presence of homogalacturonan (HG), rhamnogalacturonan I and arabinogalactan in both LLP-W and LLP-D. Notably, LLP-D was much richer in HG fraction than that of LLP-W, suggesting that the DES could assist to specifically extract HG from lotus leaves. Additionally, the lower molecular weight and higher content of uronic acids were observed in LLP-D, which might contribute to its much stronger in vitro antioxidant, hypoglycemic, and immunomodulatory effects. These findings suggest that the optimized DES assisted extraction method can be a potential approach for specific extraction of acidic polysaccharides with good bioactivities from lotus leaves for applications in the functional food industry.

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Section: Ft-ir and Nmr Spectra Of Llpsmentioning

confidence: 98%

Section: Ft-ir and Nmr Spectra Of Llpsmentioning

confidence: 99%

Section: Ft-ir and Nmr Spectra Of Llpsmentioning

confidence: 99%

See 1 more Smart Citation

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Feng

Huang

et al. 2021

Foods

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“…As shown in Figure 3d, the signals at 97.81 and 104.27 ppm were attributed to the anomeric region of the αand β-configuration, respectively. The signals at the range of 57-87 ppm indicated the existence of C2-C6 [27].…”

Section: Nmr Analysismentioning

confidence: 99%

“…One-dimensional NMR ( 1 H and 13 C NMR) is commonly used to recognize the α-or β-configuration of the anomeric proton (H1) and anomeric carbon (C1) in sugar residue [27][28][29]. In most cases, the anomeric region of α-configuration appears in 5.1-5.8 ppm (anomeric proton) and β-configuration relatively corresponds to 4.3-4.8 ppm [27]. The 4.50-3.00 ppm region was correlated to the ring proton region [30].…”

Section: Nmr Analysismentioning

confidence: 99%

Physicochemical Characterization of an Exopolysaccharide Produced by Lipomyces sp. and Investigation of Rheological and Interfacial Behavior

Guo

Chen

et al. 2021

Gels

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The present study aimed to evaluate the rheological and interfacial behaviors of a novel microbial exopolysaccharide fermented by L. starkeyi (LSEP). The structure of LSEP was measured by LC-MS, 1H and 13C NMR spectra, and FT-IR. Results showed that the monosaccharide composition of LSEP was D-mannose (8.53%), D-glucose (79.25%), D-galactose (7.15%), and L-arabinose (5.07%); there existed the anomeric proton of α-configuration and the anomeric carbon of α- and β-configuration; there appeared the characteristic absorption peak of the phosphate ester bond. The molecular weight of LSEP was 401.8 kDa. The water holding capacity (WHC, 2.10 g/g) and oil holding capacity (OHC, 12.89 g/g) were also evaluated. The results of rheological properties showed that the aqueous solution of LSEP was a non-Newtonian fluid, exhibiting the shear-thinning characteristics. The adsorption of LSEP can reduce the interfacial tension (11.64 mN/m) well and form an elastic interface layer at the MCT–water interface. Such functional properties make LSEP a good candidate for use as thickener, gelling agent, and emulsifier to form long-term emulsions for food, pharmaceutical, and cosmetic products.

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Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway

Zhao

Chang

et al. 2023

Food Science & Nutrition

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The hard‐shelled mussel (Mytilus coruscus) has been used as a traditional Chinese medicine and health food in China for centuries. Polysaccharides from mussel has been reported to have multiple biological functions, however, it remains unclear whether mussel polysaccharide (MP) exerts protective effects in intestinal functions, and the underlying mechanisms of action remain unclear. The aim of this study was to investigate the protective effects and mechanism of MP on intestinal oxidative injury in mice. In this study, 40 male BALB/C mice were used, with 30 utilized to produce an animal model of intestinal oxidative injury with intraperitoneal injection of cyclophosphamide (Cy) for four consecutive days. The protective effects of two different doses of MP (300 and 600 mg/kg) were assessed by investigating the change in body weight, visceral index, and observing colon histomorphology. Moreover, the underlying molecular mechanisms were investigated by measuring the antioxidant enzymes and related signaling molecules through ELISA, real‐time PCR, and western blot methods. The results showed that MP pretreatment effectively protected the intestinal from Cy‐induced injury: improved the colon tissue morphology and villus structure, increased superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‐Px) activities, and reduced malondialdehyde (MDA) content in serum and colon tissues. Meanwhile, MP also significantly increased the expression levels of SOD, GSH‐Px, heme oxygenase‐1 (HO‐1), and nuclear factor E2‐related factor 2 (Nrf2) mRNA in colon tissues. Further, western blot results showed that the expression of Nrf2 protein was significantly upregulated while kelch‐like ECH‐associated protein 1 (Keap1) was significantly downregulated by MP in the colonic tissues. This study indicates that MP can ameliorate Cy‐induced oxidative stress injury in mice, and Nrf2‐Keap1 signaling pathway may mediate these protective effects.

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A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective

Cited by 202 publications

References 176 publications

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Physicochemical Characterization of an Exopolysaccharide Produced by Lipomyces sp. and Investigation of Rheological and Interfacial Behavior

Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway

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