The integral equation (IE) method is one of the efficient approaches for solving electromagnetic problems, where dyadic Green’s function (DGF) plays an important role as the Kernel of the integrals. In general, a layered medium with planar, cylindrical, or spherical geometry can be used to model different biomedical media such as human skin, body, or head. Therefore, in this chapter, different approaches for the derivation of Green’s function for these structures will be introduced. Due to the recent great interest in two-dimensional (2D) materials, the chapter will also discuss the generalization of the technique to the same structures with interfaces made of isotropic and anisotropic surface impedances. To this end, general formulas for the dyadic Green’s function of the aforementioned structures are extracted based on the scattering superposition method by considering field and source points in the arbitrary locations. Apparently, by setting the surface conductivity of the interfaces equal to zero, the formulations will turn into the associated problem with dielectric boundaries. This section will also aid in the design of various biomedical devices such as sensors, cloaks, and spectrometers, with improved functionality. Finally, the Purcell factor of a dipole emitter in the presence of the layered structures will be discussed as another biomedical application of the formulation.