A review of microfabrication techniques and dielectrophoretic microdevices for particle manipulation and separation

Li, Ming; Li, Weihua; Zhang, Jun; Alici, Gürsel; Wen, Weijia

doi:10.1088/0022-3727/47/6/063001

Cited by 196 publications

(179 citation statements)

References 246 publications

(215 reference statements)

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“…Compared to the active manipulation of particles via an externally imposed force field (e.g., acoustic [17], electric [18], magnetic [19] or optical [20]), the passive manipulation exploits the flowinduced intrinsic lift and/or drag force to control particle motion with several advantages such as simplicity and autonomy [21]. Along this direction, inertial microfluidics has attracted tremendous attention since the pioneering study of Di Carlo et al [22] due to its capability to handle a large volume of samples at a high throughput [23,24].…”

Section: Introductionmentioning

confidence: 99%

Particle manipulations in non-Newtonian microfluidics: A review

Liu

et al. 2017

Journal of Colloid and Interface Science

234

192

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a b s t r a c tMicrofluidic devices have been widely used since 1990s for diverse manipulations of particles (a general term of beads, cells, vesicles, drops, etc.) in a variety of applications. Compared to the active manipulation via an externally imposed force field, the passive manipulation of particles exploits the flow-induced intrinsic lift and/or drag to control particle motion with several advantages. Along this direction, inertial microfluidics has received tremendous interest in the past decade due to its capability to handle a large volume of samples at a high throughput. This inertial lift-based approach in Newtonian fluids, however, becomes ineffective and even fails for small particles and/or at low flow rates. Recent studies have demonstrated the potential of elastic lift in non-Newtonian fluids for manipulating particles with a much smaller size and over a much wider range of flow rates. The aim of this article is to provide an overview of the various passive manipulations, including focusing, separation, washing and stretching, of particles that have thus far been demonstrated in non-Newtonian microfluidics.

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Section: Introductionmentioning

confidence: 99%

Particle manipulations in non-Newtonian microfluidics: A review

Liu

et al. 2017

Journal of Colloid and Interface Science

234

192

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“…This limitation can be solved by introducing 3D electrodes within the device. Many researchers have recently realized this aspect and proposed different fabrication techniques to fabricate embedded 3D electrodes [68]. By introducing 3D electrodes, the flow-rates and the throughput of the DEP-based devices can be enhanced although throughput of EK methods is relatively low, and at this point not suitable for clinical applications.…”

Section: Flow-rate and Throughputmentioning

confidence: 99%

“…Additional fabrication steps needs to be introduced for the devices with 3D electrodes. Different fabrication strategies for DEP-based microfluidic devices have been reviewed recently by Li et al [68]. Titanium, gold, silver and carbon are common materials for the electrodes [68].…”

Section: Materials and Fabricationmentioning

confidence: 99%

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Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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“…8 Concerning electrokinetic methods, dielectrophoresis (DEP) has been widely used as a manipulation tool for the patterning and positioning of both particles and cells. 9 DEP is one of the available cell-manipulation techniques and has been widely used for separating, 10,11 concentrating, 12 and aligning 13 cells. DEP methods are attractive due to their rapidity, massiveness and unnecessity scheme of labeling.…”

mentioning

confidence: 99%

Simple Formation of Cell Arrays Embedded in Hydrogel Sheets and Cubes

Sugano¹,

Sasaki²,

Mizutani³

et al. 2018

ANAL. SCI.

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127Cell-manipulation techniques have been developed for singlecell arrays that can be applied to high-throughput single-cell analysis for genomics and proteomics. Concerning electrokinetic methods, dielectrophoresis (DEP) has been widely used as a manipulation tool for the patterning and positioning of both particles and cells.9 DEP is one of the available cell-manipulation techniques and has been widely used for separating, 10,11 concentrating, 12 and aligning 13 cells. DEP methods are attractive due to their rapidity, massiveness and unnecessity scheme of labeling. However, the DEP force is temporary, and the disappearance of DEP regulation occurs upon switching off the voltage application, which leads to a redispersion of cells accumulated for cell patterning. Thus, immobilization techniques, such as covalent bonding via cross-linking agents, 14 microwell arrays, 15,16 cell-adhesive proteins, 17 and the encapsulation into hydrogels, [18][19][20] have been incorporated into the DEP manipulation technique to maintain patterned cells at their directed positions. The encapsulation of patterned cells into photopolymerizable hydrogel is among suitable methods to obtain a cell array with precise positioning owing to rapid and simple manners. In addition, the use of antibodies with selective recognition for target molecules has permitted one to develop a rapid and simple immunosensing system, 21,22 a discrimination system of cells expressed with target proteins on the cell membrane 23,24 and selective capturing of target bacteria. 25In this study, we used a DEP device consisting of a grid electrode to form a cell array. The cells suspended in a prepolymer solution of hydrogel are directed to the targeted position to form an island organization of cells. The gelation of prepolymer solution in the DEP device with cellular patterns was induced by irradiating ultraviolet (UV) light; hence, patterned cells embedded in the hydrogel sheets can be obtained after removing the grid electrode. Furthermore, control of the optical transparency of the grid electrode allows one to fabricate cubes with single cells and cell aggregation.A grid electrode was fabricated on an indium-tin oxide (ITO) substrate by photolithography. Figure 1A shows a schematic design of the grid electrode used as an upper substrate for the cell patterning device. An array of 10000 (100 × 100) panels with a 90-μm square was fabricated by a negative photoresist (5 μm thick, SU-8 3025, MicroChem Corp., Newton, MO) to define the grid electrode with a 10-μm width exposed to the solution. The substrate with the grid electrode was mounted on the flat ITO substrate via polyester film with a microfluidic channel (11 mm wide, 20 mm long and 60 μm thick).Huh-7 hepatoma cell lines (Huh-7 cells) were dispersed in a prepolymer solution consisting of a 20(v/v)% of poly(ethylene glycol) diacrylate (Aldrich), 1(v/v)% of 2-hydroxy-2-methylpropiophenone (Tokyo Kasei Kogyo Co. Ltd.) used as a photoinitiator, 75(v/v)% of 200 mM sucrose and 4(v/v)% of a DMEM medium. Figure 1B ...

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A review of microfabrication techniques and dielectrophoretic microdevices for particle manipulation and separation

Abstract: A review of microfabrication techniques and dielectrophoretic microdevices for A review of microfabrication techniques and dielectrophoretic microdevices for particle manipulation and separation particle manipulation and separation

Cited by 196 publications

References 246 publications

Particle manipulations in non-Newtonian microfluidics: A review

Particle manipulations in non-Newtonian microfluidics: A review

Microfluidic bio-particle manipulation for biotechnology

Simple Formation of Cell Arrays Embedded in Hydrogel Sheets and Cubes

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