A review of microfabrication and hydrogel engineering for micro-organs on chips

Verhulsel, Marine; Vignes, Maéva; Descroix, Stéphanie; Malaquin, Laurent; Vignjević, Danijela; Viovy, Jean‐Louis

doi:10.1016/j.biomaterials.2013.11.021

Cited by 205 publications

(157 citation statements)

References 131 publications

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“…After these results were derived, an analysis of recent published and peer-reviewed data showed that loss of RGD-responsive cell attachments during cryopreservation is a leading cause of cell loss (Terry et al, 2007). Furthermore, there are strong indications that sucrose as a porogen helps provide in vivo -like conditions in a step-wise process during tissue fabrication (Verhulsel et al, 2014). Subsequent to the derivation of these initial results, further work has shown that expansion and cryopreservation in 3D capsule matrices for cellular aggregates is demonstrable for human embryonic stem cells further confirming that decision choices that encompass cell attachment (similar in function to RGD-containing matrices), slow-cooling, matrix permeability, natural biomaterials (e.g., alginate) enhance cell viability (Serra et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

A Bayesian approach to optimizing cryopreservation protocols

Sambu¹

2015

PeerJ

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Cryopreservation is beset with the challenge of protocol alignment across a wide range of cell types and process variables. By taking a cross-sectional assessment of previously published cryopreservation data (sample means and standard errors) as preliminary meta-data, a decision tree learning analysis (DTLA) was performed to develop an understanding of target survival using optimized pruning methods based on different approaches. Briefly, a clear direction on the decision process for selection of methods was developed with key choices being the cooling rate, plunge temperature on the one hand and biomaterial choice, use of composites (sugars and proteins as additional constituents), loading procedure and cell location in 3D scaffolding on the other. Secondly, using machine learning and generalized approaches via the Naïve Bayes Classification (NBC) method, these metadata were used to develop posterior probabilities for combinatorial approaches that were implicitly recorded in the metadata. These latter results showed that newer protocol choices developed using probability elicitation techniques can unearth improved protocols consistent with multiple unidimensionally-optimized physical protocols. In conclusion, this article proposes the use of DTLA models and subsequently NBC for the improvement of modern cryopreservation techniques through an integrative approach.

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Section: Resultsmentioning

confidence: 99%

A Bayesian approach to optimizing cryopreservation protocols

Sambu¹

2015

PeerJ

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“…In this sense it is interesting to point out the quite relevant number of papers that appeared on the use of microenvironments for cell organization and orientation leading to micro-organ formation. A wide overview on this specific subject was very recently published by Verhulsel et al [142].…”

Section: Bone Cartilage and Skinmentioning

confidence: 99%

Introduction

Alhaique¹,

Coviello²,

Matricardi³

2015

Polysaccharide Hydrogels

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“…[71] Integrated with real-time high-resolution imaging and in vitro assays, they can provide accurate and time-varying data on the genetic and metabolic activities of living cells. [72] The "organ-on-a-chip" revolution has seen significant progress in recent years, and a few highlights are outlined below.…”

Section: Organ-on-a-chip Systemsmentioning

confidence: 99%

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Raman

Bashir

2017

Adv Healthcare Materials

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how the concept of bioinspiration, or imitating biological design and functionality in synthetic materials, created a new class of "smart" biomimetic materials. Then, we shall discuss how the continuing development of enabling manufacturing technologies, such as 3D printing and microfluidics, has established the separate subdiscipline of biofabrication for tissue engineering, or "building with biology." Finally, we shall investigate the convergence of these two fields into the emerging discipline of biohybrid design, the use of biological materials to power non-natural functional behaviors in synthetic machines. Throughout this report, we will revisit a single class of materials, hydrogels, as a case study of biological design in the context of each subfield, and discuss how the constraints, underlying principles, and end-use applications differ in each case. We will also discuss ethical considerations of biological design, with a special focus on forward engineering non-natural or hypernatural functional behaviors in biohybrid systems. We will conclude with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practices for the next generation of engineers and scientists. Biomimicry and Bioinspired DesignA deeper understanding of the underlying design principles that govern biological systems has inspired the field of biomimicry. [1,2] Observing adaptive phenomena in nature, scientists and engineers have sought to extract the components of biological design responsible for this behavior and replicate the behavior in synthetic materials. Fundamentally, this involves understanding the building blocks or base units from which a biological material is built, the hierarchical assembly of these building blocks, and the interactions and interfaces between them.In this section, we will discuss strategies that engineers and scientists have employed to engineer bioinspired hierarchy, from bottom-up self-assembly and top-down engineered assembly. We will present several key demonstrations of stimulus-responsive hydrogels ranging from the micro-to the macroscale, and investigate novel demonstrations in biomimetic actuation and movement. We will conclude with the remaining challenges in the field of biomimicry and discuss the potential future impact of bioinspired design.The discipline of biological design has a relatively short history, but has undergone very rapid expansion and development over that time. This Progress Report outlines the evolution of this field from biomimicry to biofabrication to biohybrid systems' design, showcasing how each subfield incorporates bioinspired dynamic adaptation into engineered systems. Ethical implications of biological design are discussed, with an emphasis on establishing responsible practices for engineering non-natural or hypernatural functional behaviors in biohybrid systems. This report concludes with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practice...

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A review of microfabrication and hydrogel engineering for micro-organs on chips

Cited by 205 publications

References 131 publications

A Bayesian approach to optimizing cryopreservation protocols

A Bayesian approach to optimizing cryopreservation protocols

Introduction

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

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