A review of hemostatic chemical components and their mechanisms in traditional Chinese medicine and ethnic medicine

Mu, Kailang; Liu, Yuchen; Liu, Gang; Ran, Fei; Zhou, Lingli; Wu, Yutong; Peng, Leqiang; Shao, M.; Chang-ju, LI; Zhang, Yongping

doi:10.1016/j.jep.2023.116200

Cited by 18 publications

(12 citation statements)

References 58 publications

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“…51 Meanwhile, a large number of flavonoids such as rutin, isoquercetin, and luteolin have been demonstrated to shorten the coagulation times by promoting the coagulation pathways and platelet aggregation or by regulating the vessel's capillary permeability. 13 These concepts are in line with our results with respect to flavonoid derivatives acting as potential hemostatic ligands.…”

Section: Potential Upa Ligand Inhibition Validationsupporting

confidence: 88%

“…Among other mechanisms, hemostatic drugs can avoid traumatic bleeding by activating coagulation factors and blocking fibrinolysis. 13 uPA has been reported to have a crucial role in fibrinolysis by catalyzing the conversion of plasminogen to plasmin. 31 In fibrinolysis, plasmin has an essential function, namely, cleaving fibrin at the bleeding site.…”

Section: Upa Activity Of the Three Euphorbiaceae Extractsmentioning

confidence: 99%

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Potential hemostatic compounds targeting urokinase plasminogen activator explored from three Euphorbiaceae species: Euphorbia maculata, Euphorbia humifusa, and Acalypha australis, with bio‐affinity ultrafiltration UPLC‐MS

Fan

Chen

2023

Phytochemical Analysis

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IntroductionNumerous species of the Euphorbiaceae family, including Euphorbia maculata, Euphorbia humifusa, and Acalypha australis, have been used to manage bleeding disorders. However, few investigations have demonstrated their hemostatic potential, and their procoagulant compounds remain elusive.ObjectiveThis study aimed to determine the most active procoagulant extracts from the three species' crude extract (CE) and fractions in order to screen out the active compounds and to analyze their possible mechanisms of action.MethodsAn integrative approach, comprising prothrombin time and activated partial thromboplastin time evaluations and urokinase‐type plasminogen activator (uPA) inhibitory assessment, followed by bio‐affinity ultrafiltration paired with UPLC/QTOF‐MS targeting uPA and docking simulations, was used.ResultsThe extracts with highest procoagulant activity were the CE for both E. maculata (EMCE) and E. humifusa (EHCE) and the n‐butanol fraction (NB) for A. australis (AANB). The most promising ligands, namely, isoquercetin, orientin, rutin, and brevifolin carboxylic acid, were selected from these lead extracts. All of these compounds exhibited pronounced specific binding values to the uPA target and showed tight intercalation with the crucial side chains forming the uPA active pocket, which may explain their mode of action. The activity validation substantiated their hemostatic effectivity in inhibiting uPA as they had better inhibition constant (Ki) values than the reference drug tranexamic acid.ConclusionCollectively, the integrative strategy applied to these three species allowed the elucidation of the mechanisms underlying their therapeutic effects on bleeding disorders, resulting in the fast detection of four potential hemostatic compounds and their mode of action.

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Section: Potential Upa Ligand Inhibition Validationsupporting

confidence: 88%

Section: Upa Activity Of the Three Euphorbiaceae Extractsmentioning

confidence: 99%

Potential hemostatic compounds targeting urokinase plasminogen activator explored from three Euphorbiaceae species: Euphorbia maculata, Euphorbia humifusa, and Acalypha australis, with bio‐affinity ultrafiltration UPLC‐MS

Fan

Chen

2023

Phytochemical Analysis

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“…As reported, hemostatic drugs work by increasing the number of platelets or enhancing the efficacy of platelets. [ 34 ] In this study, we found that the hemostatic effect of CPO‐CDs was achieved by increasing the number of platelets. Besides, TXA 2 and PGI 2 are a pair of endogenous substances with opposite effects on regulating platelets’ functions, the dynamic balance of which is the material basis of general hemostasis.…”

Section: Resultsmentioning

confidence: 84%

“…To effectively stop hemorrhage, the in‐depth mechanisms that cause platelets adhesion, activation, and aggregation must occur simultaneously. [ 34 ] Platelets adhesion to the extracellular matrix is the initial step in primary hemostasis. [ 45 ] Thus, to investigate the in vitro hemostasis activity of CPO‐CDs, platelets adhesion experiment was first conducted.…”

Section: Resultsmentioning

confidence: 99%

Carbonized Platycladus orientalis Derived Carbon Dots Accelerate Hemostasis through Activation of Platelets and Coagulation Pathways

Liang,

Bi,

Zhou

et al. 2023

Small

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Achieving rapid and effective hemostasis remains a multidisciplinary challenge. Here, distinctive functional carbon dots derived from carbonized Platycladus orientalis (CPO‐CDs) are developed using one‐step hydrothermal method. The negatively charged surface of CPO‐CDs retains partial functional groups from CPO precursor, exhibiting excellent water solubility and high biocompatibility. Both rat liver injury model and tail amputation model have confirmed the rapid and effective hemostatic performance of CPO‐CDs on exogenous hemorrhage. Further, on endogenous blood‐heat hemorrhage syndrome rat model, CPO‐CDs could inhibit hemorrhage and alleviate inflammation response. Interestingly, the excellent hemostasis performance of CPO‐CDs is ascribed to activate exogenous coagulation pathway and common coagulation pathway. More importantly, metabolomics of rat plasma suggests that the hemostasis effect of CPO‐CDs is closely related to platelet functions. Therefore, the designed in vitro experiments are performed and it is discovered that CPO‐CDs significantly promote platelets adhesion, activation, and aggregation. Further, the underlying mechanism investigation suggests that Src/Syk signal pathway plays a key role in platelets activation triggered by CPO‐CDs. Overall, CPO‐CDs with rapid and excellent hemostatic performance are discovered for the first time, which could be an excellent candidate for the treatment of hemorrhagic diseases.

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“…Furthermore, they can accelerate the process of physiological hemostasis by stimulating vasoconstriction, promoting the adhesion and aggregation of red blood cells (RBCs) and platelets, initiating coagulation cascades, and enhancing the strength and stability of blood clots by inhibiting fibrinolysis. 44,45 More importantly, the nanocomposite hemostatic materials can effectively overcome the inherent adverse effects of inorganic hemostatic agents made of naked mineral components.…”

Section: Introductionmentioning

confidence: 99%

Progress and future prospects of hemostatic materials based on nanostructured clay minerals

Yang,

Wang,

Yang

et al. 2023

Biomater. Sci.

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A review of hemostatic chemical components and their mechanisms in traditional Chinese medicine and ethnic medicine

Cited by 18 publications

References 58 publications

Potential hemostatic compounds targeting urokinase plasminogen activator explored from three Euphorbiaceae species: Euphorbia maculata, Euphorbia humifusa, and Acalypha australis, with bio‐affinity ultrafiltration UPLC‐MS

Potential hemostatic compounds targeting urokinase plasminogen activator explored from three Euphorbiaceae species: Euphorbia maculata, Euphorbia humifusa, and Acalypha australis, with bio‐affinity ultrafiltration UPLC‐MS

Carbonized Platycladus orientalis Derived Carbon Dots Accelerate Hemostasis through Activation of Platelets and Coagulation Pathways

Progress and future prospects of hemostatic materials based on nanostructured clay minerals

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