A review of heavy metal cation binding to deoxyribonucleic acids for the creation of chemical sensors

Kanellis, Vangelis George; Remedios, Cristobal G. dos

doi:10.1007/s12551-018-0455-y

Cited by 38 publications

(17 citation statements)

References 112 publications

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“…The loss of the cytosine signals is interpreted as a toxic effect by Al ions, although it is far less critical than copper. Al 3+ interacts with carbonyl O and/or N ring donors in nucleotide bases [35,36] and selectively binds to the backbone of the PO2 group and/or to the guanine N-7 site of the G-C base pairs by chelation [37,38].…”

Section: Raman Spectroscopymentioning

confidence: 99%

Rapid Inactivation of SARS-CoV-2 by Silicon Nitride, Copper, and Aluminum Nitride

Pezzotti

Ohgitani

Shin‐Ya

et al. 2020

Preprint

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IntroductionViral disease spread by contaminated commonly touched surfaces is a global concern. Silicon nitride, an industrial ceramic that is also used as an implant in spine surgery, has known antibacterial activity. The mechanism of antibacterial action relates to the hydrolytic release of surface disinfectants. It is hypothesized that silicon nitride can also inactivate the coronavirus SARS-CoV-2.Methods SARS-CoV-2 virions were exposed to 15 wt.% aqueous suspensions of silicon nitride, aluminum nitride, and copper particles. The virus was titrated by the TCD50 method using VeroE6/TMPRSS2 cells, while viral RNA was evaluated by real-time RT-PCR. Immunostaining and Raman spectroscopy were used as additional probes to investigate the cellular responses to virions exposed to the respective materials. ResultsAll three tested materials showed >99% viral inactivation at one and ten minutes of exposure. Degradation of viral RNA was also observed with all materials. Immunofluorescence testing showed that silicon nitride-treated virus failed to infect VeroE6/TMPRSS2 cells without damaging them. In contrast, the copper-treated virus suspension severely damaged the cells due to copper ion toxicity. Raman spectroscopy indicated differential biochemical cellular changes due to infection and metal toxicity for two of the three materials tested. ConclusionsSilicon nitride successfully inactivated the SARS-CoV-2 in this study. The mechanism of action was the hydrolysis-mediated surface release of nitrogen-containing disinfectants. Both aluminum nitride and copper were also effective in the inactivation of the virus. However, while the former compound affected the cells, the latter compound had a cytopathic effect. Further studies are needed to validate these findings and investigate whether silicon nitride can be incorporated into personal protective equipment and commonly touched surfaces, as a strategy to discourage viral persistence and disease spread.

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Section: Raman Spectroscopymentioning

confidence: 99%

Rapid Inactivation of SARS-CoV-2 by Silicon Nitride, Copper, and Aluminum Nitride

Pezzotti

Ohgitani

Shin‐Ya

et al. 2020

Preprint

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“…The final result is a Co II -compromised Cascade complex with increased nicking activity that could lead to DNA damage. Co II can also infiltrate the DNA helix and cause structural changes and breaks in the strands (Kanellis and Dos Remedios, 2018). Consistent with the need to repair damaged DNA, cells upregulated a UvrD helicase (GSU0763) of the nucleotide excision repair pathway.…”

Section: Evidence For Dna Damagementioning

confidence: 82%

Cobalt Resistance via Detoxification and Mineralization in the Iron-Reducing Bacterium Geobacter sulfurreducens

et al. 2020

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Bacteria in the genus Geobacter thrive in iron- and manganese-rich environments where the divalent cobalt cation (CoII) accumulates to potentially toxic concentrations. Consistent with selective pressure from environmental exposure, the model laboratory representative Geobacter sulfurreducens grew with CoCl2 concentrations (1 mM) typically used to enrich for metal-resistant bacteria from contaminated sites. We reconstructed from genomic data canonical pathways for CoII import and assimilation into cofactors (cobamides) that support the growth of numerous syntrophic partners. We also identified several metal efflux pumps, including one that was specifically upregulated by CoII. Cells acclimated to metal stress by downregulating non-essential proteins with metals and thiol groups that CoII preferentially targets. They also activated sensory and regulatory proteins involved in detoxification as well as pathways for protein and DNA repair. In addition, G. sulfurreducens upregulated respiratory chains that could have contributed to the reductive mineralization of the metal on the cell surface. Transcriptomic evidence also revealed pathways for cell envelope modification that increased metal resistance and promoted cell-cell aggregation and biofilm formation in stationary phase. These complex adaptive responses confer on Geobacter a competitive advantage for growth in metal-rich environments that are essential to the sustainability of cobamide-dependent microbiomes and the sequestration of the metal in hitherto unknown biomineralization reactions.

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“…The mechanism of DNA interaction with metal cations varies depending on the nature of metal cations. This can be either purely electrostatic, i.e., interaction of negatively charged DNA phosphates with metal cations, or can involve coordination of metal ions with nucleobases [31,32]. The latter mechanism dominates in DNA binding with transition metal ions and is characterized by higher binding constants [31].…”

Section: Adsorption Of Heavy Metal Ions By Dna-cs Hydrogelmentioning

confidence: 99%

“…DNA forms a strong complex with Hg 2+ ion, which binds exclusively to DNA bases and is strongly held between two DNA helices [36]. Three other metal ions interact with DNA by either electrostatic binding with phosphates or coordination with nucleobases and DNA affinity to these ions changes as Cu 2+ > Pb 2+ > Cd 2+ [32]. Cu 2+ has a higher binding constant than Pb 2+ and Cd 2+ ions owing to its higher affinity to DNA bases [37].…”

Section: Adsorption Of Heavy Metal Ions By Dna-cs Hydrogelmentioning

confidence: 99%

Adsorptive Removal of Heavy Metal Ions, Organic Dyes, and Pharmaceuticals by DNA–Chitosan Hydrogels

Chan

Morikawa

Shibata

et al. 2021

Gels

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DNA–chitosan (DNA–CS) hydrogel was prepared by in situ complexation between oppositely charged DNA and chitosan polyelectrolytes via electrostatic cross-linking to study its adsorption characteristics. The DNA–chitosan hydrogel matrix contains (i) cationic (NH3+) and anionic (PO4–) sites for electrostatic binding with ionic species, (ii) -OH and -NH2 groups and heteroaromatic DNA nucleobases for chelation of heavy metal ions, and (iii) DNA double-helix for recognition and binding to small organic molecules of various structures and polarities. DNA–CS hydrogels efficiently bind with Hg2+, Pb2+, Cd2+, and Cu2+ metal cations of significant environmental concern. Adsorption capacities of DNA–CS hydrogels for studied metal ions depend on hydrogel composition and pH of solution and reach ca. 50 mg/g at neutral pHs. Hydrogels with higher DNA contents show better adsorption characteristics and notably higher adsorption capacity to Hg2+ ions. Because of the co-existence of cationic and anionic macromolecules in the DNA–CS hydrogel, it demonstrates an affinity to both anionic (Congo Red) and cationic (Methylene Blue) dyes with moderate adsorption capacities of 12.6 mg/g and 29.0 mg/g, respectively. DNA–CS hydrogel can also be used for adsorptive removal of pharmaceuticals on conditions that their molecules are sufficiently hydrophobic and have ionogenic group(s). Facile preparation and multitarget adsorption characteristics of DNA–CS hydrogel coupled with sustainable and environmentally friendly characteristics render this system promising for environmental cleaning applications.

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A review of heavy metal cation binding to deoxyribonucleic acids for the creation of chemical sensors

Cited by 38 publications

References 112 publications

Rapid Inactivation of SARS-CoV-2 by Silicon Nitride, Copper, and Aluminum Nitride

Rapid Inactivation of SARS-CoV-2 by Silicon Nitride, Copper, and Aluminum Nitride

Cobalt Resistance via Detoxification and Mineralization in the Iron-Reducing Bacterium Geobacter sulfurreducens

Adsorptive Removal of Heavy Metal Ions, Organic Dyes, and Pharmaceuticals by DNA–Chitosan Hydrogels

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