A review of enhancers for chemiluminescence enzyme immunoassay

Chen, Ge; Jin, Maojun; Du, Pengfei; Zhang, Chan; Cui, Xueyan; Zhang, Yudan; Wang, Jing; Jin, Fen; She, Yongxin; Shao, Hua; Wang, Shanshan; Zheng, Lirong

doi:10.1080/09540105.2016.1272550

Cited by 41 publications

(27 citation statements)

References 40 publications

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“…The methods developed in this study had detection limits for A. simplex antigens in food of 0.5 ng/mL (CL-S-ELISA) and 5 ng/mL (CL-C-ELISA). The use of chemiluminescence technology has been shown to allow for more sensitive analyte detection than a colorimetric assay by up to five orders of magnitude [ 41 ]. Indeed, this was also the case in the present study, in which a 1,2-dioxetane-based chemiluminescence substrate was used for the sensitive detection of alkaline phosphatase (AP).…”

Section: Discussionmentioning

confidence: 99%

Development and Application of Novel Chemiluminescence Immunoassays for Highly Sensitive Detection of Anisakis simplex Proteins in Thermally Processed Seafood

et al. 2020

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The third-stage larvae (L3) of Anisakis simplex are the most important source of hidden allergens in seafood products. However, there exist no commercial methods for detecting Anisakis proteins in food. Furthermore, only a few methods have been validated for the detection of A. simplex in thermally processed food. The aims of our study are (i) the development and validation of high-sensitivity chemiluminescent (CL) immunoassays for the detection of A. simplex proteins in processed seafood, (ii) and A. simplex antigen detection in common seafood products from Polish markets. We developed and validated CL sandwich ELISA (S-ELISA) and CL competitive ELISA (C-ELISA) methods for A. simplex proteins detection in food, with respective detection limits of 0.5 and 5 ng/mL. The usefulness of the assays for detecting A. simplex proteins in highly processed food was evaluated by examination of autoclaved canned fish spiked with A. simplex larvae (1–8 larvae/200 g). Commercial real-time PCR was unable to detect A. simplex in autoclaved samples at all levels of enrichment with Anisakis larvae. CL-S-ELISA was used to test various types of seafood products from Polish markets. Among all tested products (n = 259), 28% were positive. A. simplex antigens were found mostly (n = 39) in smoked fish products: mackerel, herring, cod, and hake. Other positive samples were found in marinated herrings, canned cod livers, canned mackerels, and surimi sticks. In tuna, Atlantic argentine, anchovy, sardine, sprat, and squid products, A. simplex antigens were not detected. This study provides novel effective tools for the detection of A. simplex proteins in processed food and highlights the potential allergic hazards for Anisakis-sensitized Polish consumers of seafood.

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Section: Discussionmentioning

confidence: 99%

Development and Application of Novel Chemiluminescence Immunoassays for Highly Sensitive Detection of Anisakis simplex Proteins in Thermally Processed Seafood

et al. 2020

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“…Chemiluminescence is characterized by the emission of light as the result of a chemical reaction. Chemiluminescence, in contrast to bioluminescence, is a non-enzymatic phenomenon in which a chemical reaction produces a high energy intermediate that returns to its ground state emitting light provided molecular oxygen or hydrogen peroxide is present [ 54 , 63 , 64 ].…”

Section: Classification Of Synthetic Enzyme Substratesmentioning

confidence: 99%

“…The rate of chemiexcitation of the phenolate-dioxetane upon deprotection highly influences the sensitivity of the analytical bioassay since the signal/background rate is higher when photons are released within a short period of time [ 64 ]. Therefore, probes where a faster chemiexcitation occurred were designed by including a styryl moiety in place of the acryl one, which chemiexcitation showed to be two orders of magnitude faster due to the formation of a stabilised phenoxyl radical.…”

Section: Classification Of Synthetic Enzyme Substratesmentioning

confidence: 99%

Modified Enzyme Substrates for the Detection of Bacteria: A Review

Pala¹,

Širec²,

Spitz³

2020

Molecules

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The ability to detect, identify and quantify bacteria is crucial in clinical diagnostics, environmental testing, food security settings and in microbiology research. Recently, the threat of multidrug-resistant bacterial pathogens pushed the global scientific community to develop fast, reliable, specific and affordable methods to detect bacterial species. The use of synthetically modified enzyme substrates is a convenient approach to detect bacteria in a specific, economic and rapid manner. The method is based on the use of specific enzyme substrates for a given bacterial marker enzyme, conjugated to a signalogenic moiety. Following enzymatic reaction, the signalophor is released from the synthetic substrate, generating a specific and measurable signal. Several types of signalophors have been described and are defined by the type of signal they generate, such as chromogenic, fluorogenic, luminogenic, electrogenic and redox. Signalophors are further subdivided into groups based on their solubility in water, which is key in defining their application on solid or liquid media for bacterial culturing. This comprehensive review describes synthetic enzyme substrates and their applications for bacterial detection, showing their mechanism of action and their synthetic routes.

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“…Moreover, the high sensitivity, and wide linear range make CL-based assays applicable in different scientific and industrial areas [ 2 ]. Enzymes are used in most CL methods as a catalyst, such as horseradish peroxidase and alkaline phosphatase [ 3 ]. However, enzymes have the disadvantages of short lifetime and low stability, which means they are easily denaturalized.…”

Section: Introductionmentioning

confidence: 99%

Integration of 3D Hydrodynamic Focused Microreactor with Microfluidic Chemiluminescence Sensing for Online Synthesis and Catalytical Characterization of Gold Nanoparticles

Wang

Seidel

2021

Sensors

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Chemiluminescence assays have shown great advantages compared with other optical techniques. Gold nanoparticles have drawn much attention in chemiluminescence analysis systems as an enzyme-free catalyst. The catalytic activity of gold nanoparticles for chemiluminescence sensing depends on size, shape and the surface charge property, which is hard to characterize in batches. As there is no positive or negative correlation between chemiluminescence signals and sizes of gold nanoparticles, the best way to get optimal gold nanoparticles is to control the reaction conditions via online chemiluminescence sensing systems. Therefore, a new method was developed for online synthesis of gold nanoparticles with a three-dimension hydrodynamic focusing microreactor, directly coupled with a microfluidic chemiluminescence sensing chip, which was coupled to a charge-coupled device camera for direct catalytical characterization of gold nanoparticles. All operations were performed in an automatic way with a program controlled by Matlab. Gold nanoparticles were synthesized through a single-phase reaction using glucose as a reducing agent and stabilizer at room temperature. The property of gold nanoparticles was easily controlled with the three-dimension microreactor during synthesis. The catalyst property of synthesized gold nanoparticles was characterized in a luminol–NaOCl chemiluminescence system. After optimizing parameters of synthesis, the chemiluminescence signal was enhanced to a factor of 171. The gold nanoparticles synthesized under optimal conditions for the luminol–NaOCl system were stable for at least one month. To further investigate the catalytic activity of synthesized gold nanoparticles in various situations, two methods were used to change the property of gold nanoparticles. After adding a certain amount of salt (NaCl), gold nanoparticles aggregated with a changed surface charge property and the catalytic activity was greatly enhanced. Glutathione was used as an example of molecules with thiol groups which interact with gold nanoparticles and reduce the catalytic activity. The chemiluminescence intensity was reduced by 98.9%. Therefore, we could show that using a microreactor for gold nanoparticles synthesis and direct coupling with microfluidic chemiluminescence sensing offers a promising monitoring method to find the best synthesis condition of gold nanoparticles for catalytic activity.

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A review of enhancers for chemiluminescence enzyme immunoassay

Cited by 41 publications

References 40 publications

Development and Application of Novel Chemiluminescence Immunoassays for Highly Sensitive Detection of Anisakis simplex Proteins in Thermally Processed Seafood

Development and Application of Novel Chemiluminescence Immunoassays for Highly Sensitive Detection of Anisakis simplex Proteins in Thermally Processed Seafood

Modified Enzyme Substrates for the Detection of Bacteria: A Review

Integration of 3D Hydrodynamic Focused Microreactor with Microfluidic Chemiluminescence Sensing for Online Synthesis and Catalytical Characterization of Gold Nanoparticles

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