A review of contemporary practice and proficiency with Gram staining in anatomical pathology laboratories

Peck, Martyn; Badrick, Tony

doi:10.1080/01478885.2017.1327474

Cited by 6 publications

(4 citation statements)

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“…The Gram methods for sections usually include dyes intended to show tissue architecture in addition to the micro-organisms. Martyn Peck presents findings from a study conducted for the Royal College of Pathologists of Australasia Quality Assurance Programs, comparing participants' results with three popular staining procedures applied to paraffin sections of a standard preparation containing known bacteria [13]. This paper usefully includes photomicrographs of badly stained preparations.…”

Section: Fibers Bacteria and Things That Look Like Fungi Under The Microscopementioning

confidence: 99%

Fibers, bacteria and things that look like fungi under the microscope

Kiernan

2017

Journal of Histotechnology

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Section: Fibers Bacteria and Things That Look Like Fungi Under The Microscopementioning

confidence: 99%

Fibers, bacteria and things that look like fungi under the microscope

Kiernan

2017

Journal of Histotechnology

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“…Over the years, Gram staining has been modified for many times, such as the Brown-Hopps method, Brown-Brenn method, and Gram-Twort method ( Brown and Brenn 1931 ; Brown and Hopps 1973 ; Peck and Badrick 2017 ), and these approaches as mentioned earlier are widely used in anatomical pathology laboratories. Through the comparison of various improved methods, it is found that Gram’s original four-step method is still used, and some researchers have adopted the three-step method, while its basic principle has not been changed.…”

mentioning

confidence: 99%

A Novel Improved Gram Staining Method Based on the Capillary Tube

Yang

2020

Polish Journal of Microbiology

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In this work, an exploratory study was conducted to examine Gram staining based on the capillary tube. Each Gram staining step for all bacterial strains tested was completed in capillary tubes. The results showed that different Gram staining morphologies were clearly visible in the capillary tubes. The results presented here demonstrated that the improved method could effectively distinguish between Gram-positive and Gram-negative bacteria, and only small volumes of reagents were required in this method. Collectively, this efficient method could rapidly and accurately identify the types of bacteria. Therefore, our findings could be used as a useful reference study for other staining methods.

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“…Bacteria can usually be broadly categorized into the gram positive and gram negative ( Figure 1). [3][4] The two bacterial types with different cell wall structures, display distinctively different cell envelope properties and abilities. Gram staining is a simple technique widely employed to differentiate both types of bacteria.…”

Section: Bacterial Cell Structurementioning

confidence: 99%

“…More importantly, BlaC enzymes are responsible for the deadly tuberculosis diseases due to its high activity, resistance and survival mechanism. 64 One of the key attributes to inhibition of this class of β-lactamases with the exception of carbapenemases, is the decrease of the steric hindrance at R, R 1 , R 3 , and R 4 of the β-lactam antibiotics (Figure 3). [65][66] This decrease in bulkiness of the antibiotic ensures the successful accommodation of the drug side chain into the enzymatic active pocket, thereby leading to effectual blockage of the β-lactamase binding pocket.…”

Section: Ambler Class Amentioning

confidence: 99%

Development of small molecule probe for screening of bacterial resistant enzyme and their biomedical applications

Chan¹

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I would like to thank Assoc. Prof. Xing Bengang for his guidance during my M.Sc. research. I am thankful to Junxin and other group members for their support and assistance during my period of research. I would like to express my gratitude towards Prof. Chiba Shunsuke for his approval of his laboratory usage for the synthetic work, which greatly facilitated this research. I am also extremely thankful towards Ciputra, Derek, Sherman, Hiro, and others for their constant guidance and support from my undergraduate to graduate period of studies. I would like to thank Jin Yong for his contributions during his final year project, to the work presented in this thesis. I would like to express my appreciation to the staffs from the Division of Chemistry and Biological Chemistry (CBC), Ee Ling and Wen Wei for their assistance with instrument trainings/usages; Janice, Si Yun, and Yean Chin for their assistance in graduate studies matters. I would like to express my gratitude to Nanyang Technological University for the financial support during the original Ph.D. candidature, in the form of Nanyang Research Scholarship. I would also like to thank CBC for giving me the opportunity to attend the 2 nd International Biophotonics Conference, Singapore. Last but not least, I would like to thank my daddy and mummy for their support in my decisions regarding further studies. I am also extremely grateful to my friends, Gillian, Wei Ting, Wei Li, and others for their constant support and encouragement. ii

show abstract

A review of contemporary practice and proficiency with Gram staining in anatomical pathology laboratories

Cited by 6 publications

References 1 publication

Fibers, bacteria and things that look like fungi under the microscope

Fibers, bacteria and things that look like fungi under the microscope

A Novel Improved Gram Staining Method Based on the Capillary Tube

Development of small molecule probe for screening of bacterial resistant enzyme and their biomedical applications

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