A review of bioanalytical methods for the therapeutic drug monitoring of β-lactam antibiotics in critically ill patients: Evaluation of the approaches used to develop and validate quality attributes

Caro, Yanis; Cámara, Marı́a S.; Zan, María Mercedes de

doi:10.1016/j.talanta.2019.120619

Cited by 20 publications

(10 citation statements)

References 81 publications

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“…83 In addition, some review articles that focused on establishing LC-MS/MS and sometimes HPLC methods, particularly for beta-lactam antibiotics, have been published recently, and the readers can refer to these studies for a systematic overview. [84][85][86] Regarding TDM of anti-infective drugs, the feasible option for advanced consolidation of the analysis of a very broad spectrum of molecules has specifically attracted considerable attention. On the one hand, therapeutic protocols frequently include the parallel use of multiple drugs for a single patient, and on the other hand, the optimization of therapy for various pathogens is an ongoing process; ultimately, patients undergo analyses for several different drugs that must be performed as quickly as possible.…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Therapeutic Drug Monitoring of Antibiotic Drugs: The Role of the Clinical Laboratory

Shipkova

Jamoussi

2022

Therapeutic Drug Monitoring

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Background: Therapeutic drug monitoring (TDM) of antiinfective drugs is an increasingly complex field, given that in addition to the patient and drug as 2 usual determinants, its success is driven by the pathogen. Pharmacodynamics is related both to the patient (toxicity) and bacterium (efficacy or antibiotic susceptibility). The specifics of TDM of antimicrobial drugs stress the need for multidisciplinary knowledge and expertise, as in any other field. The role and the responsibility of the laboratory in this interplay are both central and multifaceted. This narrative review highlights the role of the clinical laboratory in the TDM process.Methods: A literature search was conducted in PubMed and Google Scholar, focusing on the past 5 years (studies published since 2016) to limit redundancy with previously published review articles. Furthermore, the references cited in identified publications of interest were screened for additional relevant studies and articles. Results:The authors addressed microbiological methods to determine antibiotic susceptibility, immunochemical and chromatographic methods to measure drug concentrations (primarily in blood samples), and endogenous clinical laboratory biomarkers to monitor treatment efficacy and toxicity. The advantages and disadvantages of these methods are critically discussed, along with existing gaps and future perspectives on strategies to provide clinicians with as reliable and useful results as possible.Conclusions: Although interest in the field has been the driver for certain progress in analytical technology and quality in recent years, laboratory professionals and commercial providers persistently encounter numerous unresolved challenges. The main tasks that need tackling include broadly and continuously available, easily operated, and cost-effective tests that offer short turnaround times, combined with reliable and easy-to-interpret results. Various fields of research are currently addressing these features.

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Section: Chromatographic Methodsmentioning

confidence: 99%

Therapeutic Drug Monitoring of Antibiotic Drugs: The Role of the Clinical Laboratory

Shipkova

Jamoussi

2022

Therapeutic Drug Monitoring

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“…The majority of in‐house methods for β‐lactam quantification utilize chromatographic separation coupled to ultraviolet (UV) or mass spectrometric (MS) detection 70,71 . The ideal assay method requires a lower limit of quantification that is lower than the MIC values of common pathogens as well as an upper limit of quantification of ≥100 mg/L so that repeat assay with need for dilution is avoided 70 .…”

Section: Operationalizing Tdmmentioning

confidence: 99%

A guide to therapeutic drug monitoring of β‐lactam antibiotics

2021

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Pharmacists play an important role in assessing, managing, and monitoring the efficacy and safety of medications. A common avenue for accomplishing this responsibility is through therapeutic drug monitoring (TDM) of many drugs, a duty that has been successfully managed by pharmacists for over four decades. 1,2 Antimicrobials, including aminoglycosides, vancomycin, voriconazole, and historically, chloramphenicol and quinidine, have been frequent candidates for pharmacist-led TDM. Antimicrobial TDM has traditionally targeted drugs with narrow therapeutic indices, where prevention of toxicity was the

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“…Numerous studies have been published for the TDM of antibiotics, mainly about liquid chromatography. Many quantification methods using liquid chromatography coupled with UV detection were developed, but selectivity must be properly evaluated as interference may occur when patients are polymedicated [20]. Due to the improvement of technology, better selectivity, precision, and sensitivity were achieved with liquid chromatography coupled with mass spectrometry (LC-MS).…”

Section: Introductionmentioning

confidence: 99%

Quantification of 15 Antibiotics Widely Used in the Critical Care Unit with a LC-MS/MS System: An Easy Method to Perform a Daily Therapeutic Drug Monitoring

et al. 2021

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Potential under- or overdose of antibiotics may occur in intensive care units due to high variability in plasma concentrations. The risk is either treatment failure or toxicity. Thus, therapeutic drug monitoring of antibiotics may guide dosing adjustment, maximising antibacterial efficacy and minimising toxicity. The aim of this study was to develop and validate a method for the analysis of 15 antibiotics including beta-lactams, linezolid, fluoroquinolones, daptomycin, and clindamycin to have a complete panel in the management of infections. We proposed to develop a fast, sensitive, and quantitative method for the analysis of 15 antibiotics using ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometer (UPLC-MS/MS) technology. this method required only 100 µL of plasma and consisted of a rapid liquid–liquid deproteinisation using methanol. Calibration curves ranged from 0.078 to 500 mg/L depending on the molecules, and were defined according to a therapeutic range. Inter- and intra-assay precisions values were less than 15%. This work described the development and the full validation of a precise, sensitive and accurate assay using UPLC-MS/MS technology. After validation, this new assay was successfully applied to routine therapeutic drug monitoring.

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A review of bioanalytical methods for the therapeutic drug monitoring of β-lactam antibiotics in critically ill patients: Evaluation of the approaches used to develop and validate quality attributes

Cited by 20 publications

References 81 publications

Therapeutic Drug Monitoring of Antibiotic Drugs: The Role of the Clinical Laboratory

Therapeutic Drug Monitoring of Antibiotic Drugs: The Role of the Clinical Laboratory

A guide to therapeutic drug monitoring of β‐lactam antibiotics

Quantification of 15 Antibiotics Widely Used in the Critical Care Unit with a LC-MS/MS System: An Easy Method to Perform a Daily Therapeutic Drug Monitoring

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