A review of allograft ophthalmic tissue in eye surgery

Baddon, Andrew C.; Jones, Mark; Armitage, W. John; Warwick, Ruth M.; Kaye, Stephen B.

doi:10.1007/s10561-009-9159-7

Cited by 10 publications

(5 citation statements)

References 3 publications

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“…This leads to a dramatic improvement in a patient's quality of life, economic opportunities, and independence. 10 The organization of eye donation and retrieval in the UK has improved considerably in recent years. The Ocular Tissue Advisory Group of NHSBT, made up of representatives of ophthalmologists from each region in the UK, the eye banks, and other interested parties such as the Royal College of Ophthalmologists, provide formal advice and guidance to NHSBT regarding standards for ocular tissue donation and transplantation in the UK and the development of safe and equitable selection and allocation criteria.…”

Section: Corneal Transplantation In the Ukmentioning

confidence: 99%

Tissue and corneal donation and transplantation in the UK

Gaum

Reynolds

Jones

et al. 2012

British Journal of Anaesthesia

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NHS Blood and Transplant (NHSBT) was established in 2005 as a Special Health Authority when the National Blood Authority and UK Transplant merged. This helped to bring tissue banking and organ transplantation services under one umbrella organization. This merger means that ~!95% of all deceased donors (whether tissue, organ or both) are now facilitated by one organization. NHSBT Tissue Services is the largest tissue establishment in the UK, and is a multi-tissue bank that specializes in the consent, retrieval, processing, storage, and dispatch of donated tissue coordinated from a purpose built, state-of-the-art tissue bank in Liverpool. Tissue donations can come from either tissue-only donors or solid organ donors who also donate tissue. Annually there are ~450 multi-tissue donors and 2500 eye donors in the UK, resulting in many thousands of transplants, including 3564 cornea transplants in 2010-2011. The separation of tissue- and organ-specific donors is largely artificial, and while organ transplantation can be life-saving, tissue transplantation can also have a dramatic effect on a patient's quality of life. It is hoped that all donors, both organ and tissue, will be recognized for the gift they make to society after their death.

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Section: Corneal Transplantation In the Ukmentioning

confidence: 99%

Tissue and corneal donation and transplantation in the UK

Gaum

Reynolds

Jones

et al. 2012

British Journal of Anaesthesia

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“…In physician‐based ophthalmology, keratoplasty is the most common surgical procedure to restore corneal integrity or transparency . Nowadays, according to the World Health Organization, more than 120 000 corneal transplants are performed each year worldwide, being one of the most common solid tissue transplanted ( http://www.who.int/ethics/topics/human_transplant_report/en/).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation (−20 °C) of feline corneoscleral tissue: histologic, microbiologic, and ultrastructural study

Costa

Leiva

Naranjo

et al. 2016

Veterinary Ophthalmology

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Cryopreservation of feline corneoscleral tissue seems to reduce bacterial contamination over time. Apoptosis is the main cause of death of cryopreserved feline keratocytes. Based on the lack of significant structural differences between STC and LTC samples, these cryopreserved tissues could potentially be used for tectonic support for at least 10 years without structural or microbiological impediment.

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“…Corneal transplantation is the most used surgical procedure to restore corneal integrity and transparency in human ophthalmology . Conversely, and despite the good surgical results reported so far, keratoplasties are underused in domestic animals .…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation (−20°C) of equine corneoscleral tissue: Microbiological, histological, and ultrastructural study

Costa

Leiva

Naranjo³

et al. 2019

Veterinary Ophthalmology

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Objective To evaluate microbiological, histological, and ultrastructural characteristics of short‐term cryopreserved (STC) equine corneoscleral tissue (<1 year), and to compare it with long‐term cryopreserved (LTC) tissue (>7 years). Animals studied Thirty‐four healthy equine globes. Procedure After a decontamination protocol, globes were enucleated and stored at −20°C in broad‐spectrum antibiotics. Corneoscleral tissue was evaluated at different storage periods: 1 month‐1 year (20 eyes) and 7‐9 years (12 eyes). Two eyes were used as controls. Microbiologic study included direct (blood, McConkey, and Sabouraud agars) and enrichment (brain‐heart infusion broth) cultures. Cryopreservation artifacts were evaluated by hematoxylin‐eosin. Corneoscleral collagen organization and number of normal and dead keratocytes were established by transmission electron microscopy. Results All microbiologic direct cultures were negative. Enrichment cultures were positive in 12.5% of corneal and 59.4% of scleral tissues (pcornea = 0.136; psclera = 1.000). Cryopreservation artifacts were most commonly observed in LTC tissues (P = 0.002). Normal keratocytes were predominant in STC corneas (STC 60% and LTC 0%) and apoptotic ones in LTC (STC 40% and LTC 90%), whereas necrotic keratocytes were only seen in LTC (LTC 10%) (P = 0.001). No structural differences were detected in collagen organization between STC and LTC (pcornea = 1.000; psclera = 0.703). Conclusions Cryopreservation of equine corneoscleral tissue did not yield direct bacterial contamination. Apoptosis is the main cause of death of cryopreserved equine keratocytes. Based on the lack of significant structural differences between STC and LTC samples, these cryopreserved tissues could potentially be used for tectonic support for at least 9 years without structural or microbiological impediment.

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A review of allograft ophthalmic tissue in eye surgery

Cited by 10 publications

References 3 publications

Tissue and corneal donation and transplantation in the UK

Tissue and corneal donation and transplantation in the UK

Cryopreservation (−20 °C) of feline corneoscleral tissue: histologic, microbiologic, and ultrastructural study

Cryopreservation (−20°C) of equine corneoscleral tissue: Microbiological, histological, and ultrastructural study

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