A review of advances in two-dimensional thin-layer chromatography

Macroporous Polymer Monoliths in Thin Layer Format

“…In a simple variant of two-dimensional (2-D) TLC, the separation is performed in two directions via 90 • rotating the layer and using another mobile phase [96]. In more complicated approaches the layers combining two different chemistries are applied.…”

Application of targeted 2D planar chromatography in the control of ginkgolic acids in some herbal drugs and dietary supplements

“…However, the separation of ginkgolic acids was impeded by the accompanied constituents that made the quantitative analysis impossible. For this reason, based on the literature data and our own experiences (29,30), targeted 2D planar chromatography technique combining concentrating, purification, and separation of sample's constituents was used in further studies. Maintaining orthogonal rules, separation of ginkgolic acids was conducted consecutively on TLC Si60 F 254s and HPTLC RP18 WF 254s glass plates with the use of the best mobile phases described above (Figs.…”

“…The main obstacle in the determination of the composition of herbal extracts is the complex nature that hinders the separation and the determination of single components. Therefore, more and more sophisticated procedures, including multidimensional separation techniques, were employed (29,30). Among them, targeted 2D planar chromatography (2D-TLC), focused on the separation of a single compound or a group of compounds, is the most selective (29).…”

“…In this technique, a part of the plate containing the partly separated compound is cut off and the compound is transferred to the second stationary phase for subsequent analysis. The separation can be performed on one or different adsorbents (29)(30)(31)(32). If the separation is carried out on different adsorbents, both bilayer plates (33) or graft TLC techniques (34) can be used.…”

“…If the separation is carried out on different adsorbents, both bilayer plates (33) or graft TLC techniques (34) can be used. Unless the number of types of bilayer TLC plates available on the market is limited, graft TLC allows any types of adsorbents to be combined and thus appears to be the most useful (29)(30)(31)(32)35).…”