A review for cancer treatment with mushroom metabolites through targeting mitochondrial signaling pathway: In vitro and in vivo evaluations, clinical studies and future prospects for mycomedicine

Nandi, Sudeshna; Sikder, Rimpa; Rapior, Sylvie; Arnould, Stéphanie; Simal-Gandara, Jesus; Acharya, Krishnendu

doi:10.1016/j.fitote.2023.105681

Cited by 6 publications

(3 citation statements)

References 203 publications

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“…Further, annexin/propidium iodide staining evaluated with FACS analysis indicates a strong increase in both early and late apoptosis upon miconazole 30 µM treatment for 24 h. Under this respect, differences between A375 and SK-MEL-28 may depend on their different growth kinetics. A mitochondrial-dependent apoptosis mediated by caspase3/7 is well known in several pathogenic setups [63][64][65][66], further supporting the role mitochondria play in the antiproliferative effects reported in the present study. Therefore, the collected evidences indicate that miconazole induces apoptosis in melanoma, likely via an early mitochondria disregulation evident at 6 h treatment.…”

Section: Discussionsupporting

confidence: 89%

Anti-Melanoma Effects of Miconazole: Investigating the Mitochondria Involvement

Scatozza,

Giardina,

Valente

et al. 2024

IJMS

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Miconazole is an antimycotic drug showing anti-cancer effects in several cancers. However, little is known on its effects in melanoma. A375 and SK-MEL-28 human melanoma cell lines were exposed to miconazole and clotrimazole (up to 100 mM). Proliferation, viability with MTT assay and vascular mimicry were assayed at 24 h treatment. Molecular effects were measured at 6 h, namely, ATP-, ROS-release and mitochondria-related cytofluorescence. A metabolomic profile was also investigated at 6 h treatment. Carnitine was one of the most affected metabolites; therefore, the expression of 29 genes involved in carnitine metabolism was investigated in the public platform GEPIA2 on 461 melanoma patients and 558 controls. After 24 h treatments, miconazole and clotrimazole strongly and significantly inhibited proliferation in the presence of 10% serum on either melanoma cell lines; they also strongly reduced viability and vascular mimicry. After 6 h treatment, ATP reduction and ROS increase were observed, as well as a significant reduction in mitochondria-related fluorescence. Further, in A375, miconazole strongly and significantly altered expression of several metabolites including carnitines, phosphatidyl-cholines, all amino acids and several other small molecules, mostly metabolized in mitochondria. The expression of 12 genes involved in carnitine metabolism was found significantly modified in melanoma patients, 6 showing a significant impact on patients’ survival. Finally, miconazole antiproliferation activity on A375 was found completely abrogated in the presence of carnitine, supporting a specific role of carnitine in melanoma protection toward miconazole effect, and was significantly reversed in the presence of caspases inhibitors such as ZVAD-FMK and Ac-DEVD-CHO, and a clear pro-apoptotic effect was observed in miconazole-treated cells, by FACS analysis of Annexin V-FITC stained cells. Miconazole strongly affects proliferation and other biological features in two human melanoma cell lines, as well as mitochondria-related functions such as ATP- and ROS-release, and the expression of several metabolites is largely dependent on mitochondria function. Miconazole, likely acting via carnitine and mitochondria-dependent apoptosis, is therefore suggested as a candidate for further investigations in melanoma treatments.

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Section: Discussionsupporting

confidence: 89%

Anti-Melanoma Effects of Miconazole: Investigating the Mitochondria Involvement

Scatozza,

Giardina,

Valente

et al. 2024

IJMS

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show abstract

“…L. mongolica comprises numerous bioactive components, including sterols, phenolic compounds, terpenoids, and polysaccharides ( Wang et al, 2020 ). The utilization of natural compounds and from mushroom pharmaceuticals has a rich historical background within traditional medical systems, and its potential advantages in managing symptoms of cancer have garnered significant attention ( Nandi et al, 2024 ). Also, Natural chemicals may improve conventional therapies while reducing side effects ( Han et al, 1999 )( Joseph et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

The effect mechanism of ergosterol from the nutritional mushroom Leucocalocybe mongolica in breast cancer cells: Protein expression modulation and metabolomic profiling using UHPLC-ESI-Q

Hussein Zaki,

Haiying,

Mohany

et al. 2024

Saudi Pharmaceutical Journal

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“…5,6 Studies have yielded positive ndings in terms of decreasing cancer cell proliferation, inducing apoptosis, and lowering tumor development. [7][8][9][10] Mushrooms are frequently used as a supplement or adjuvant therapy to conventional cancer medications, with the potential to reduce treatmentrelated side effects and improve general well-being. 11,12 Breast cancer is a major public health issue worldwide due to its high prevalence, mortality rates, disparities in access to care, and emotional impact on individuals, families, communities, and healthcare systems.…”

Section: Introductionmentioning

confidence: 99%