A reversible surface functionalized nanowire transistor to study protein–protein interactions

Hsieh

Proc. Natl. Acad. Sci. U.S.A.

et al. 2009

Self Cite

119

In this study, we describe a highly sensitive and reusable silicon nanowire field-effect transistor for the detection of protein-protein interactions. This reusable device was made possible by the reversible association of glutathione S-transferase-tagged calmodulin with a glutathione modified transistor. The calmodulin-modified transistor exhibited selective electrical responses to Ca 2þ (≥1 μM) and purified cardiac troponin I (∼7 nM); the change in conductivity displayed a linear dependence on the concentration of troponin I in a range from 10 nM to 1 μM. These results are consistent with the previously reported concentration range in which the dissociation constant for the troponin I-calmodulin complex was determined. The minimum concentration of Ca 2þ required to activate calmodulin was determined to be 1 μM. We have also successfully demonstrated that the N-type Ca 2þ channels, expressed by cultured 293T cells, can be recognized specifically by the calmodulin-modified nanowire transistor. This sensitive nanowire transistor can serve as a high-throughput biosensor and can also substitute for immunoprecipitation methods used in the identification of interacting proteins.calcium ion | glutathione S-transfrease | N-type calcium channel | silicon nanowire field-effect transistor

Section: Resultsmentioning

confidence: 99%

Label-free detection of protein-protein interactions using a calmodulin-modified nanowire transistor

Hsieh

Proc. Natl. Acad. Sci. U.S.A.

et al. 2009

Self Cite

119

Functional Nanomaterials and Devices for Electronics, Sensors and Energy Harvesting

“…7). For instance, Lin et al [44] used an immobilization procedure based on the reversible reaction between glutathione (GSH) and glutathione S-transferase. The bond between the proteins was found to rupture in the presence of 10 mM of GSH.…”

Section: Protein Detection In Blood Serummentioning

confidence: 99%

“…The bond between the proteins was found to rupture in the presence of 10 mM of GSH. As probing molecules immobilized on NW surface, Lin et al [44] used biotinylized GSH directed antibodies that helped to perform multiple streptavidin registration.…”

Section: Protein Detection In Blood Serummentioning

confidence: 99%

SOI-Nanowire Biosensors for High-Sensitivity Protein and Gene Detection

Ivanov

Pleshakova

Popov

et al. 2014

The development of genome and proteomic technologies is related, first of all, to the progress recently achieved in developing of high-sensitivity, rapid methods for registration and analysis of nucleic acids and proteins. One of such methods, combining high sensitivity and high response speed, is the nanowire (NW) detection technique. This technique allows one to register analyte species in real time without using labels. Nowadays, the concentration sensitivity of such systems in registering proteins and DNAs reaches the femtomolar level. In the present chapter, we consider nanowire sensor systems based on silicon nanowire field-effect transistors. We discuss the achieved potential in detecting low-copy proteins with the help of nanowire systems. We show that it is possible to use not only antibodies, but also aptamers, as probing molecules for biospecific protein detection. We also discuss the possibility of using nanowire detectors in genome studies.

SpringerBriefs in Molecular Science

“…2, sizes [21], shapes [28], and dopants [29] of SiNWs can be precisely tailored with good reproducibility via various established synthetic strategies, greatly facilitating the fabrication of SiNWs-based FET with high sensitivity and multiplex capability [30]. Till now, various SiNW-FET nanosensor devices have been designed for detecting a variety of species, including nucleic acids [31][32][33][34][35][36][37][38][39][40][41], proteins [15,[42][43][44][45][46][47], chemical species and metal ions [15,[48][49][50][51], single virus particles [52], cells [53], and even interaction between molecules [54][55][56] and cell's life activities [57][58][59]. Moreover, during the past several years, great efforts have been paid to the comprehensive understanding of the filed effect in SiNWs sensors, and advance widespread adoption of the technology for routine use.…”

Section: Sinws-based Field-effect Transistormentioning

confidence: 99%

“…Besides detection of nucleic acids and proteins, other kinds of biological species and activity can also be readily detected using SiNW-FET devices, such as metal ions [15,48], small molecules [50,51], virus [52], cells [53], molecular interactions [54,55], and cell's life activities [57][58][59]. Herein, we give a simple description of these applications as follows.…”

Section: Other Applicationsmentioning

confidence: 99%

Silicon-Based Platform for Biosensing Applications

He¹,

Su²

2014

Development of high-performance biosensors vastly facilitates the analysis and detection of various biological species, including nucleic acids, protein, cell, etc. Functional nanomaterials (e.g., silver/gold nanoparticles, carbon nanotubes, graphene, silicon nanowires, etc) serve as new platform for design of nano-biosensors featuring high sensitivity and specificity. Taking advantage of the attractive merits of silicon nanowires (SiNWs) (e.g., unique electronic/optical properties, huge surface-to-volume rations, surface tailorability, fast response and good reproducibility, and compatibility with conventional silicon technology), SiNWs have been widely employed for constructing various kinds of electrochemical and optical biosensors, enabling ultrasensitive, specific, and reproducible detection of DNA and protein. We introduce a number of typical SiNWs-based biosensors (e.g., field-effect transistor (FET), amperometric-, surface-enhanced Raman scattering (SERS), and fluorescence-based biosensors) in this chapter, aiming to summarize the representative progresses of this research field in recent years. These kinds of high-quality silicon-based sensors show potentially great promise for myriad practical applications, such as medical diagnosis, food safety, drug security, environment monitoring, as well as anti-bioterrorism and so forth.Keywords Silicon nanowires Á Biosensor Á Field effect transistor Á Surfaceenhanced Raman scattering (SERS) Á DNA and protein detection Á Sensitivity and specificity Biological/chemical analysis and detection are of essential importance for disease diagnosis, drug discovery, food safety, environmental protection, and anti-bioterrorism, etc. While a large number of bioassay kits have been available on the market, there are ever-growing efforts to develop new detection methods with ultrahigh sensitivity and specificity, to meet the increasing demands of biosensing applications. Parallel to research efforts devoted to such high-end requirements, much recent interest has been directed toward the development of low-cost and portable biosensors, which possess comparable high sensitivity to conventional instruments, but with greatly reduced cost/mass/power requirements. Nanostructures (e.g., nanoparticles [1][2][3][4][5][6][7][8][9], nanotubes [10][11][12][13][14], and nanowires [15][16][17][18], etc) Y.