A reversed phase high‐performance liquid chromatography method for determination of cephalexin in human plasma

Abstract

SummaryA reversed-phase HPLC method for the analysis of cephalexin (74 (aminophenylacetyl)amino]-3-methyl-8-oxo-S-thia-l-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid) by isocratic separation is described. A comparison is made between the ultrafiltration and the extraction procedure developed in our laboratory. The extraction procedure, based on the deproteinization of plasma with perchloric acid followed by the extraction with dichloroethane and separation of cephalexin on a reversed phase column, gave better recovery. Quantitative validation of our method has been performed by an external standard technique. The relative standard deviations were between 1.1 and 1.9% in the within-day assay and between 1.2 and 2.2% in the inter-day assay. The limit of detection was 0.14 kg/ml and the limit of quantification was 0.28 kg/ml. This method is rapid, sensitive, simple, and reproducible. The LC MS/MS technique was successfully used for identification and quantification of cephalexin in human plasma samples.

Three-ways crossover bioequivalence study of cephalexin in healthy Malay volunteers

“…Hence the power of study might not be sufficient. Pecavar et al published analytical method to quantify cephalexin in human plasma; however, the method was not applied in any pharmacokinetic study or bioequivalence study 7 . Mohamed et al had conducted a bioequivalence study of three Cephalexin formulations in 24 Sudanese volunteers.…”

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“…[16] Cephalexin is official in IP, [17] United States Pharmacopoeia, [18] and BP. [19] Literature survey revealed that spectrophotometric, [20][21][22] HPLC, [23][24][25][26][27] and one HPLC method [28] have been reported for this combination drug.…”

One-step synthesis of carbon nanodots for sensitive detection of cephalexin

“…Therefore, developing effective analytical methods for the sensitive and selective detection of trace amounts of cephalexin is especially important. Compared with many other methods, like highperformance liquid chromatography (HPLC), 3 liquid chromatography (LC), 4 ow injection analysis, 5 electrochemical methods, 6 uorescence assays have several advantages, such as high sensitivity and fast analysis. However, to the best of our knowledge, the use of uorescence probe for analysis of cephalexin is very rare.…”