A Retrospective Study on Extended Spectrum Beta-Lactamase  Bacteria in the Philippines from 1999 – 2013

Lota, Maria Margarita M.; Latorre, Angelica Anne E.

doi:10.47895/amp.v48i1.1186

Cited by 2 publications

(2 citation statements)

References 31 publications

(74 reference statements)

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“…β-lactam resistance in the bacteria spreads in the last few years with no apparent treatment; that is why the infectious diseases became a major challenge in the medical field (Alekshun and Levy, 2007;Lota and Latorre, 2014;Avatsingh et al, 2023). Consecutive generations have high resistance to the β-lactams that is observed by many pathogenic bacteria and the Enterobacteriaceae in particular, such as E. coli; Enterobacter spp., Morganella spp., Proteus spp., Providentia spp., Klebsiella spp., and Serratia spp.…”

Section: Discussionmentioning

confidence: 99%

Novel approach to overcome the β-lactam resistant bacteria using an actinobacterial inhibitory protein

A. M. Abdelbary,

F. Gamal,

M. Abdulkhair

et al. 2023

Novel Research in Microbiology Journal

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β-lactam resistance is a serious problem that the hospitals face worldwide; particularly in the developing countries. The widespread of this resistance is attributed to various mechanisms used by the nosocomial bacteria. The aims of this study were to monitor the spread of the β-lactam resistant bacteria in the different provinces of Egypt; to create a biocontrol strategy by producing the β-lactamase inhibitory protein from the Streptomyces bacteria, and to knowing its suitability for the human use. Seventy β-lactam resistant bacterial isolates were sampled randomly from several hospital laboratories across ten governorates of Egypt. The isolates were screened against six different antibiotics; mainly Amoxicillin; Amoxicillin-Clavulanate, Penicillin, Ampicillin-Sulbactam, Cefepime, and Piperacillin-Tazobactam at 250 µg/ ml, and their Minimum inhibitory concentration (MIC) was recorded. The Bn67 isolate was the most promising isolate, which was molecularly identified using 16SrRNA partial sequence as Pseudomonas aeruginosa (LC710315.1). So in order to overcome this bacterial resistance; eighty actinobacteria were isolated from several soil samples collected from Giza governorate, Egypt, and were screened for their effectiveness against Bn67. The actinobacterial isolate (Stn-01) showed the maximum inhibitory efficacy against Bn67 and was identified using 16SrRNA partial sequence as Streptomyces katsurahamanus (LC710314.1). The inhibitor protein (β-LIP-n) was isolated; precipitated, and purified to give 35 kDa with 17 amino acid sequences. The β-LIPn exhibited no cytotoxic potential against the Human Skin Fibroblast (HSF) cell line at 200 µg/ ml. This approach of using the bacterial soil-based inhibitor protein to biocontrol the β-lactam resistant bacteria is considered as novel and as a promising start-up to using the environmental bacteria to overcome this problem of β-lactam resistance.

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Section: Discussionmentioning

confidence: 99%

Novel approach to overcome the β-lactam resistant bacteria using an actinobacterial inhibitory protein

A. M. Abdelbary,

F. Gamal,

M. Abdulkhair

et al. 2023

Novel Research in Microbiology Journal

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“…The findings of the study revealed that all ESBL-EC presented multidrug resistance which is common for these organisms as plasmids that carry genes that code for ESBLs also commonly contain other genes that encode for mechanisms of resistance to other antimicrobial drugs such as quinolones, aminoglycosides, chloramphenicol. (30,31) Therefore, infections caused by ESBL-producing Enterobacteriaceae are difficult to treat which may entail a significant increase in the burden of nosocomial infections. (32) Moreover, resistance was observed against each "Access" group antibiotic with the exception of AMK.…”

Section: Esbl-encoding Genes (Bla Ctxm Bla Tem and Bla Shv ) In E Co...mentioning

confidence: 99%

Presence of blaCTXM-1, blaCTXM-9, and blaTEM-1 Genes in Extended-spectrum β-lactamase-producing Escherichia coli Isolates from Hospital Wastewater

Tolenada,

Dayrit

2023

Indones Biomed J

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BACKGROUND: Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) are selectively proliferated in the human gut, excreted through feces, and deposited through wastewater lines, with hospital wastewater acting as a major reservoir of antibiotic resistance genes and resistant bacteria, thus pose adverse effects to human health. This study aimed to determine the presence of blaCTXM-1, blaCTXM-9, blaTEM-1, and blaSHV-1 genes in ESBL-EC in wastewater from selected hospitals in Manila and Quezon City, the Philippines.METHODS: Influent and effluent in twelve hospital wastewater treatment plants were collected, screened for cefotaxime-resistant E. coli, and examined for the ESBL production through phenotypic characterization using conventional bacterial identification, disk diffusion method, and VITEK® 2 Compact system and genotypic identification of ESBL-EC blaCTXM-1, blaCTXM-9, blaTEM-1, blaSHV-1 genes using multiplex polymerase chain reaction (PCR).RESULTS: Conventional bacterial identification methods and the VITEK® 2 Compact system results showed that both influent and effluent samples were positive for ESBL-EC at 33.3% and 16.7%, respectively. Multiplex PCR results revealed that various E. coli isolates were of ESBL-EC blaCTXM-1, blaCTXM-9, and blaTEM-1 genes. Multi-drug resistance was observed among all ESBL-EC isolates with resistance being highest against ampicillin, cefuroxime, ceftazidime, ceftriaxone, cefepime, piperacillin, and aztreonam.CONCLUSION: As the study revealed the presence of ESBL-producing bacteria, efforts must be made to ensure the prudent antimicrobial use with possible emphasis on antibiotic rotation accompanied by intensified infection prevention and control in hospital settings.KEYWORDS: antimicrobial resistance, beta-lactams, blaCTXM, blaTEM, extended-spectrum beta-lactamase, E. coli, hospital wastewater

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A Retrospective Study on Extended Spectrum Beta-Lactamase Bacteria in the Philippines from 1999 – 2013

Abstract: ...

Cited by 2 publications

References 31 publications

Novel approach to overcome the β-lactam resistant bacteria using an actinobacterial inhibitory protein

Novel approach to overcome the β-lactam resistant bacteria using an actinobacterial inhibitory protein

Presence of blaCTXM-1, blaCTXM-9, and blaTEM-1 Genes in Extended-spectrum β-lactamase-producing Escherichia coli Isolates from Hospital Wastewater

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